OCA at 07:26, 9 November 2022

2022-11-09T07:26:30Z

←Older revision		Revision as of 07:26, 9 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7x2a is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==MERS-CoV spike complex with S41 neutralizing antibody Fab Class1 (1u2d RBD with 1Fab)==
		+	<StructureSection load='7x2a' size='340' side='right'caption='[[7x2a]], [[Resolution\|resolution]] 2.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7x2a]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Middle_East_respiratory_syndrome-related_coronavirus Middle East respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X2A FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x2a OCA], [https://pdbe.org/7x2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x2a RCSB], [https://www.ebi.ac.uk/pdbsum/7x2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x2a ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K0BRG7_MERS K0BRG7_MERS] Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.[HAMAP-Rule:MF_04099] Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099] Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neutralizing monoclonal antibodies (mAbs) against highly pathogenic coronaviruses represent promising candidates for clinical intervention. Here, we isolated a potent neutralizing monoclonal antibody, MERS-S41, from a yeast displayed scFv library using the S protein as a bait. To uncover the neutralization mechanism, we determined structures of MERS-S41 Fab in complex with the trimeric spike glycoprotein by cryoelectron microscopy (cryo-EM). We observed four distinct classes of the complex structure, which showed that the MERS-S41 Fab bound to the "up" receptor binding domain (RBD) with full saturation and also bound to an accessible partially lifted "down" RBD, providing a structural basis for understanding how mAbs bind to trimeric spike glycoproteins. Structure analysis of the epitope and cell surface staining assays demonstrated that virus entry is blocked predominantly by direct competition with the host receptor, dipeptidyl peptidase-4 (DPP4).

-	~~Authors~~:	+	Cryoelectron microscopy structures of a human neutralizing antibody bound to MERS-CoV spike glycoprotein.,Zhang S, Jia W, Zeng J, Li M, Wang Z, Zhou H, Zhang L, Wang X Front Microbiol. 2022 Sep 28;13:988298. doi: 10.3389/fmicb.2022.988298., eCollection 2022. PMID:36246239<ref>PMID:36246239</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7x2a" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Middle East respiratory syndrome-related coronavirus]]
		+	[[Category: Wang XW]]
		+	[[Category: Zeng JW]]
		+	[[Category: Zhang SY]]
		+	[[Category: Zhou HX]]

OCA at 09:08, 18 May 2022

2022-05-18T09:08:21Z

←Older revision		Revision as of 09:08, 18 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7x2a is ON HOLD	+	The entry 7x2a is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7x2a" [edit=sysop:move=sysop]

2022-03-02T05:18:11Z

Protected "7x2a" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 2 March 2022

OCA: New page: '''Unreleased structure''' The entry 7x2a is ON HOLD Authors: Description: Category: Unreleased Structures

2022-03-02T05:18:10Z

New page: '''Unreleased structure''' The entry 7x2a is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7x2a is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7x2a - Revision history

OCA at 07:26, 9 November 2022

OCA at 09:08, 18 May 2022

OCA: Protected "7x2a" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7x2a is ON HOLD Authors: Description: Category: Unreleased Structures