7xeo - Revision history

OCA at 06:13, 2 March 2023

2023-03-02T06:13:15Z

←Older revision		Revision as of 06:13, 2 March 2023
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	==MbetaCD treated state of mTRPV2==		==MbetaCD treated state of mTRPV2==
-	<StructureSection load='7xeo' size='340' side='right'caption='[[7xeo]]' scene=''>	+	<StructureSection load='7xeo' size='340' side='right'caption='[[7xeo]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XEO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7xeo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XEO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xeo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xeo OCA], [https://pdbe.org/7xeo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xeo RCSB], [https://www.ebi.ac.uk/pdbsum/7xeo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xeo ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xeo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xeo OCA], [https://pdbe.org/7xeo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xeo RCSB], [https://www.ebi.ac.uk/pdbsum/7xeo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xeo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRPV2_MOUSE TRPV2_MOUSE] Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.<ref>PMID:10559903</ref> <ref>PMID:11707512</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The transient receptor potential vanilloid 2 (TRPV2) ion channel is a polymodal receptor widely involved in many physiological and pathological processes. Despite many TRPV2 modulators being identified, whether and how TRPV2 is regulated by endogenous lipids remains elusive. Here, we report an endogenous cholesterol molecule inside the vanilloid binding pocket (VBP) of TRPV2, with a 'head down, tail up' configuration, resolved at 3.2 A using cryo-EM. Cholesterol binding antagonizes ligand activation of TRPV2, which is removed from VBP by methyl-beta-cyclodextrin (MbetaCD) as resolved at 2.9 A. We also observed that estradiol (E2) potentiated TRPV2 activation by 2-aminoethoxydiphenyl borate (2-APB), a classic tool compound for TRP channels. Our cryo-EM structures (resolved at 2.8-3.3 A) further suggest how E2 disturbed cholesterol binding and how 2-APB bound within the VBP with E2 or without both E2 and endogenous cholesterol, respectively. Therefore, our study has established the structural basis for ligand recognition of the inhibitory endogenous cholesterol and excitatory exogenous 2-APB in TRPV2.
		+
		+	Structural mechanisms of TRPV2 modulation by endogenous and exogenous ligands.,Su N, Zhen W, Zhang H, Xu L, Jin Y, Chen X, Zhao C, Wang Q, Wang X, Li S, Wen H, Yang W, Guo J, Yang F Nat Chem Biol. 2023 Jan;19(1):72-80. doi: 10.1038/s41589-022-01139-8. Epub 2022 , Sep 26. PMID:36163384<ref>PMID:36163384</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7xeo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Nan SN~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Su N]]

OCA at 06:23, 18 August 2022

2022-08-18T06:23:36Z

←Older revision		Revision as of 06:23, 18 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7xeo ~~is ON HOLD until Paper Publication~~	+	==MbetaCD treated state of mTRPV2==
-		+	<StructureSection load='7xeo' size='340' side='right'caption='[[7xeo]]' scene=''>
-	~~Authors~~: ~~Nan~~, S.N.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XEO FirstGlance]. <br>
-	~~Description: MbetaCD treated state of mTRPV2~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xeo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xeo OCA], [https://pdbe.org/7xeo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xeo RCSB], [https://www.ebi.ac.uk/pdbsum/7xeo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xeo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Nan~~, S.N~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Nan SN]]

OCA at 04:54, 12 May 2022

2022-05-12T04:54:45Z

←Older revision		Revision as of 04:54, 12 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xeo is ON HOLD	+	The entry 7xeo is ON HOLD until Paper Publication

	Authors: Nan, S.N.		Authors: Nan, S.N.

OCA: Protected "7xeo" [edit=sysop:move=sysop]

2022-04-06T09:16:41Z

Protected "7xeo" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:16, 6 April 2022

OCA: New page: '''Unreleased structure''' The entry 7xeo is ON HOLD Authors: Nan, S.N. Description: MbetaCD treated state of mTRPV2 Category: Unreleased Structures Category: Nan, S.N

2022-04-06T09:16:40Z

New page: '''Unreleased structure''' The entry 7xeo is ON HOLD Authors: Nan, S.N. Description: MbetaCD treated state of mTRPV2 Category: Unreleased Structures Category: Nan, S.N

New page

'''Unreleased structure'''

The entry 7xeo is ON HOLD

Authors: Nan, S.N.

Description: MbetaCD treated state of mTRPV2
[[Category: Unreleased Structures]]
[[Category: Nan, S.N]]