OCA at 10:05, 14 December 2022

2022-12-14T10:05:24Z

←Older revision		Revision as of 10:05, 14 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7xht is ~~ON HOLD~~	+	==Structure of the OgeuIscB-omega RNA-target DNA complex==
		+	<StructureSection load='7xht' size='340' side='right'caption='[[7xht]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xht]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xht OCA], [https://pdbe.org/7xht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xht RCSB], [https://www.ebi.ac.uk/pdbsum/7xht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xht ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transposon-encoded IscB family proteins are RNA-guided nucleases in the OMEGA (obligate mobile element-guided activity) system, and likely ancestors of the RNA-guided nuclease Cas9 in the type II CRISPR-Cas adaptive immune system. IscB associates with its cognate omegaRNA to form a ribonucleoprotein complex that cleaves double-stranded DNA targets complementary to an omegaRNA guide segment. Although IscB shares the RuvC and HNH endonuclease domains with Cas9, it is much smaller than Cas9, mainly due to the lack of the alpha-helical nucleic-acid recognition lobe. Here, we report the cryo-electron microscopy structure of an IscB protein from the human gut metagenome (OgeuIscB) in complex with its cognate omegaRNA and a target DNA, at 2.6-A resolution. This high-resolution structure reveals the detailed architecture of the IscB-omegaRNA ribonucleoprotein complex, and shows how the small IscB protein assembles with the omegaRNA and mediates RNA-guided DNA cleavage. The large omegaRNA scaffold structurally and functionally compensates for the recognition lobe of Cas9, and participates in the recognition of the guide RNA-target DNA heteroduplex. These findings provide insights into the mechanism of the programmable DNA cleavage by the IscB-omegaRNA complex and the evolution of the type II CRISPR-Cas9 effector complexes.

-	~~Authors~~:	+	Structure of the IscB-omegaRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9.,Kato K, Okazaki S, Kannan S, Altae-Tran H, Esra Demircioglu F, Isayama Y, Ishikawa J, Fukuda M, Macrae RK, Nishizawa T, Makarova KS, Koonin EV, Zhang F, Nishimasu H Nat Commun. 2022 Nov 7;13(1):6719. doi: 10.1038/s41467-022-34378-3. PMID:36344504<ref>PMID:36344504</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7xht" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Metagenome]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Isayama Y]]
		+	[[Category: Ishikawa J]]
		+	[[Category: Kato K]]
		+	[[Category: Nishimasu H]]
		+	[[Category: Nishizawa T]]
		+	[[Category: Okazaki O]]

OCA: Protected "7xht" [edit=sysop:move=sysop]

2022-04-21T01:42:36Z

Protected "7xht" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:42, 21 April 2022

OCA: New page: '''Unreleased structure''' The entry 7xht is ON HOLD Authors: Description: Category: Unreleased Structures

2022-04-21T01:42:35Z

New page: '''Unreleased structure''' The entry 7xht is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7xht is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7xht - Revision history

OCA at 10:05, 14 December 2022

OCA: Protected "7xht" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7xht is ON HOLD Authors: Description: Category: Unreleased Structures