7yed - Revision history

OCA at 06:24, 7 April 2023

2023-04-07T06:24:23Z

←Older revision		Revision as of 06:24, 7 April 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7yed is ~~ON HOLD until Paper~~ Publication	+	==In situ structure of polymerase complex of mammalian reovirus in the elongation state==
		+	<StructureSection load='7yed' size='340' side='right'caption='[[7yed]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7yed]] is a 25 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YED FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=UTP:URIDINE+5-TRIPHOSPHATE'>UTP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yed OCA], [https://pdbe.org/7yed PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yed RCSB], [https://www.ebi.ac.uk/pdbsum/7yed PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yed ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C9E874_9VIRU C9E874_9VIRU]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian reovirus (reovirus) is a multilayered, turreted member of Reoviridae characterized by transcription of dsRNA genome within the innermost capsid shell. Here, we present high-resolution in situ structures of reovirus transcriptase complex in an intact double-layered virion, and in the uncoated single-layered core particles in the unloaded, reloaded, pre-elongation, and elongation states, respectively, obtained by cryo-electron microscopy and sub-particle reconstructions. At the template entry of RNA-dependent RNA polymerase (RdRp), the RNA-loading region gets flexible after uncoating resulting in the unloading of terminal genomic RNA and inactivity of transcription. However, upon adding transcriptional substrates, the RNA-loading region is recovered leading the RNAs loaded again. The priming loop in RdRp was found to play a critical role in regulating transcription, which hinders the elongation of transcript in virion and triggers the rearrangement of RdRp C-terminal domain (CTD) during elongation, resulting in splitting of template-transcript hybrid and opening of transcript exit. With the integration of these structures, a transcriptional model of reovirus with five states is proposed. Our structures illuminate the RdRp activation and regulation of the multilayered turreted reovirus.

-	~~Authors: Bao~~, K~~.Y.~~, Zhang, X~~.L.~~, Li, D~~.Y.~~, ~~Zhu~~, P.	+	In situ structures of polymerase complex of mammalian reovirus illuminate RdRp activation and transcription regulation.,Bao K, Zhang X, Li D, Sun W, Sun Z, Wang J, Zhu P Proc Natl Acad Sci U S A. 2022 Dec 13;119(50):e2203054119. doi: , 10.1073/pnas.2203054119. Epub 2022 Dec 5. PMID:36469786<ref>PMID:36469786</ref>

-	~~Description: In situ structure~~ of ~~polymerase complex of mammalian reovirus in~~ the ~~elongation state~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Bao~~, K.Y~~]]	+	<div class="pdbe-citations 7yed" style="background-color:#fffaf0;"></div>
-	[[Category: Li~~, D.Y~~]]	+	== References ==
-	[[Category: Zhang~~, X.L~~]]	+	<references/>
-	[[Category: Zhu, P]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mammalian orthoreovirus 3]]
		+	[[Category: Bao KY]]
		+	[[Category: Li DY]]
		+	[[Category: Zhang XL]]
		+	[[Category: Zhu P]]

OCA at 04:39, 29 March 2023

2023-03-29T04:39:06Z

←Older revision		Revision as of 04:39, 29 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yed is ON HOLD	+	The entry 7yed is ON HOLD until Paper Publication

	Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.		Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.
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	Description: In situ structure of polymerase complex of mammalian reovirus in the elongation state		Description: In situ structure of polymerase complex of mammalian reovirus in the elongation state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zhang, X.L]]
	[[Category: Bao, K.Y]]		[[Category: Bao, K.Y]]
-	[[Category: Zhu, P]]
	[[Category: Li, D.Y]]		[[Category: Li, D.Y]]
		+	[[Category: Zhang, X.L]]
		+	[[Category: Zhu, P]]

OCA at 07:25, 15 March 2023

2023-03-15T07:25:59Z

←Older revision		Revision as of 07:25, 15 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yed is ON HOLD ~~until Paper Publication~~	+	The entry 7yed is ON HOLD

	Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.		Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.

OCA at 08:22, 15 February 2023

2023-02-15T08:22:33Z

←Older revision		Revision as of 08:22, 15 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yed is ON HOLD	+	The entry 7yed is ON HOLD until Paper Publication

	Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.		Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.

OCA at 04:51, 8 February 2023

2023-02-08T04:51:22Z

←Older revision		Revision as of 04:51, 8 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yed is ON HOLD ~~until Paper Publication~~	+	The entry 7yed is ON HOLD

	Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.		Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.

OCA at 03:32, 25 August 2022

2022-08-25T03:32:33Z

←Older revision		Revision as of 03:32, 25 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yed is ON HOLD	+	The entry 7yed is ON HOLD until Paper Publication

	Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.		Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.
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	Description: In situ structure of polymerase complex of mammalian reovirus in the elongation state		Description: In situ structure of polymerase complex of mammalian reovirus in the elongation state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Bao, K.Y]]
-	[[Category: Li, D.Y]]
	[[Category: Zhang, X.L]]		[[Category: Zhang, X.L]]
		+	[[Category: Bao, K.Y]]
	[[Category: Zhu, P]]		[[Category: Zhu, P]]
		+	[[Category: Li, D.Y]]

OCA: Protected "7yed" [edit=sysop:move=sysop]

2022-07-13T04:02:18Z

Protected "7yed" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:02, 13 July 2022

OCA: New page: '''Unreleased structure''' The entry 7yed is ON HOLD Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P. Description: In situ structure of polymerase complex of mammalian reovirus in th...

2022-07-13T04:02:17Z

New page: '''Unreleased structure''' The entry 7yed is ON HOLD Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P. Description: In situ structure of polymerase complex of mammalian reovirus in th...

New page

'''Unreleased structure'''

The entry 7yed is ON HOLD

Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P.

Description: In situ structure of polymerase complex of mammalian reovirus in the elongation state
[[Category: Unreleased Structures]]
[[Category: Bao, K.Y]]
[[Category: Li, D.Y]]
[[Category: Zhang, X.L]]
[[Category: Zhu, P]]