7yjb - Revision history

OCA at 18:10, 29 November 2023

2023-11-29T18:10:15Z

←Older revision		Revision as of 18:10, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7yjb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Odontosoria_chusana Odontosoria chusana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJB FirstGlance]. <br>		<table><tr><td colspan='2'>[[7yjb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Odontosoria_chusana Odontosoria chusana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=ERD:(2S)-2-(3,4-DIHYDROXYPHENYL)-5,7-DIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>ERD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=ERD:(2S)-2-(3,4-DIHYDROXYPHENYL)-5,7-DIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>ERD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yjb OCA], [https://pdbe.org/7yjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yjb RCSB], [https://www.ebi.ac.uk/pdbsum/7yjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yjb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yjb OCA], [https://pdbe.org/7yjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yjb RCSB], [https://www.ebi.ac.uk/pdbsum/7yjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yjb ProSAT]</span></td></tr>
	</table>		</table>
Line 16:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 7yjb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7yjb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chalcone synthase\|Chalcone synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:35, 3 November 2022

2022-11-03T07:35:08Z

←Older revision		Revision as of 07:35, 3 November 2022
Line 1:		Line 1:

	==Crystal structure of Stenoloma chusanum chalcone synthase 1 (ScCHS1) complex with CoA and Eriodictyol==		==Crystal structure of Stenoloma chusanum chalcone synthase 1 (ScCHS1) complex with CoA and Eriodictyol==
-	<StructureSection load='7yjb' size='340' side='right'caption='[[7yjb]]' scene=''>	+	<StructureSection load='7yjb' size='340' side='right'caption='[[7yjb]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7yjb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Odontosoria_chusana Odontosoria chusana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yjb OCA], [https://pdbe.org/7yjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yjb RCSB], [https://www.ebi.ac.uk/pdbsum/7yjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yjb ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=ERD:(2S)-2-(3,4-DIHYDROXYPHENYL)-5,7-DIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>ERD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yjb OCA], [https://pdbe.org/7yjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yjb RCSB], [https://www.ebi.ac.uk/pdbsum/7yjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yjb ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The key enzymes involved in the flavonoid biosynthesis pathway have been extensively studied in seed plants, but relatively less in ferns. In this study, two 4-Coumarate: coenzyme A ligases (Sc4CL1 and Sc4CL2) and one novel chalcone synthase (ScCHS1) were functionally characterized by mining the Stenoloma chusanum transcriptome database. Recombinant Sc4CLs were able to esterify various hydroxycinnamic acids to corresponding acyl-coenzyme A (CoA). ScCHS1 could catalyze p-coumaroyl-CoA, cinnamoyl-CoA, caffeoyl-CoA, and feruloyl-CoA to form naringenin, pinocembrin, eriodictyol, and homoeriodictyol, respectively. Moreover, enzymatic kinetics studies revealed that the optimal substrates of ScCHS1 were feruloyl-CoA and caffeoyl-CoA, rather than p-coumaroyl-CoA, which was substantially different from the common CHSs. Crystallographic and site-directed mutagenesis experiments indicated that the amino acid residues, Leu87, Leu97, Met165, and Ile200, located in the substrate-binding pocket near the B-ring of products, could exert a significant impact on the unique catalytic activity of ScCHS1. Furthermore, overexpression of ScCHS1 in tt4 mutants could partially rescue the mutant phenotypes. Finally, ScCHS1 and Sc4CL1 were used to synthesize flavanones and flavones with multi-substituted hydroxyl and methoxyl B-ring in Escherichia coli, which can effectively eliminate the need for the cytochrome P450 hydroxylation/O-methyltransferase from simple phenylpropanoid acids. In summary, the identification of these important Stenoloma enzymes provides a springboard for the future production of various flavonoids in E. coli. This article is protected by copyright. All rights reserved.
		+
		+	Molecular and structural characterization of a promiscuous chalcone synthase from the fern species Stenoloma chusanum.,Ni R, Niu M, Fu J, Tan H, Zhu TT, Zhang J, Lou HX, Zhang P, Li JX, Cheng AX J Integr Plant Biol. 2022 Aug 3. doi: 10.1111/jipb.13335. PMID:35920566<ref>PMID:35920566</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7yjb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Odontosoria chusana]]
	[[Category: Cheng AX]]		[[Category: Cheng AX]]
	[[Category: Li JX]]		[[Category: Li JX]]
	[[Category: Zhang P]]		[[Category: Zhang P]]

OCA at 04:52, 25 August 2022

2022-08-25T04:52:17Z

←Older revision		Revision as of 04:52, 25 August 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7yjb is ON HOLD~~	+	==Crystal structure of Stenoloma chusanum chalcone synthase 1 (ScCHS1) complex with CoA and Eriodictyol==
-		+	<StructureSection load='7yjb' size='340' side='right'caption='[[7yjb]]' scene=''>
-	~~Authors: Li, J.X., Cheng, A.X., Zhang, P.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJB FirstGlance]. <br>
-	~~Description:~~ Crystal structure of Stenoloma chusanum chalcone synthase 1 (ScCHS1) complex with CoA and Eriodictyol	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yjb OCA], [https://pdbe.org/7yjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yjb RCSB], [https://www.ebi.ac.uk/pdbsum/7yjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yjb ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cheng~~, A.X~~]]	+	__TOC__
-	[[Category: Li~~, J.X~~]]	+	</StructureSection>
-	[[Category: Zhang, P]]	+	[[Category: Large Structures]]
		+	[[Category: Cheng AX]]
		+	[[Category: Li JX]]
		+	[[Category: Zhang P]]

OCA: Protected "7yjb" [edit=sysop:move=sysop]

2022-07-27T16:27:44Z

Protected "7yjb" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:27, 27 July 2022

OCA: New page: '''Unreleased structure''' The entry 7yjb is ON HOLD Authors: Li, J.X., Cheng, A.X., Zhang, P. Description: Crystal structure of Stenoloma chusanum chalcone synthase 1 (ScCHS1) complex...

2022-07-27T16:27:42Z

New page: '''Unreleased structure''' The entry 7yjb is ON HOLD Authors: Li, J.X., Cheng, A.X., Zhang, P. Description: Crystal structure of Stenoloma chusanum chalcone synthase 1 (ScCHS1) complex...

New page

'''Unreleased structure'''

The entry 7yjb is ON HOLD

Authors: Li, J.X., Cheng, A.X., Zhang, P.

Description: Crystal structure of Stenoloma chusanum chalcone synthase 1 (ScCHS1) complex with CoA and Eriodictyol
[[Category: Unreleased Structures]]
[[Category: Cheng, A.X]]
[[Category: Li, J.X]]
[[Category: Zhang, P]]