OCA at 14:03, 18 October 2023

2023-10-18T14:03:57Z

←Older revision		Revision as of 14:03, 18 October 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ysr is ~~ON HOLD until 2025~~-02-12	+	==GTPgammaS MT decorated with kinesin==
		+	<StructureSection load='7ysr' size='340' side='right'caption='[[7ysr]], [[Resolution\|resolution]] 4.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7ysr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YSR FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ysr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ysr OCA], [https://pdbe.org/7ysr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ysr RCSB], [https://www.ebi.ac.uk/pdbsum/7ysr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ysr ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TBA1_DROME TBA1_DROME] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In eukaryotes, the dynamic assembly of microtubules (MT) plays an important role in numerous cellular processes. The underlying mechanism of GTP triggering MT assembly is still unknown. Here, we present cryo-EM structures of tubulin heterodimer at their GTP- and GDP-bound states, intermediate assembly states of GTP-tubulin, and final assembly stages of MT. Both GTP- and GDP-tubulin heterodimers adopt similar curved conformations with subtle flexibility differences. In head-to-tail oligomers of tubulin heterodimers, the inter-dimer interface of GDP-tubulin exhibits greater flexibility, particularly in tangential bending. Cryo-EM of the intermediate assembly states reveals two types of tubulin lateral contacts, "Tube-bond" and "MT-bond". Further, molecular dynamics (MD) simulations show that GTP triggers lateral contact formation in MT assembly in multiple sequential steps, gradually straightening the curved tubulin heterodimers. Therefore, we propose a flexible model of GTP-initiated MT assembly, including the formation of longitudinal and lateral contacts, to explain the nucleation and assembly of MT.

-	~~Authors: Zhou~~, J., Wang, H.-W.	+	Structural insights into the mechanism of GTP initiation of microtubule assembly.,Zhou J, Wang A, Song Y, Liu N, Wang J, Li Y, Liang X, Li G, Chu H, Wang HW Nat Commun. 2023 Sep 25;14(1):5980. doi: 10.1038/s41467-023-41615-w. PMID:37749104<ref>PMID:37749104</ref>

-	~~Description~~: ~~GTPgammaS MT decorated with kinesin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Wang, H.-W]]	+	<div class="pdbe-citations 7ysr" style="background-color:#fffaf0;"></div>
-	[[Category: Zhou, J]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Drosophila melanogaster]]
		+	[[Category: Large Structures]]
		+	[[Category: Wang H-W]]
		+	[[Category: Zhou J]]

OCA at 08:49, 2 August 2023

2023-08-02T08:49:19Z

←Older revision		Revision as of 08:49, 2 August 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ysr is ON HOLD until ~~2024~~-08-12	+	The entry 7ysr is ON HOLD until 2025-02-12

	Authors: Zhou, J., Wang, H.-W.		Authors: Zhou, J., Wang, H.-W.

OCA at 06:25, 21 September 2022

2022-09-21T06:25:30Z

←Older revision		Revision as of 06:25, 21 September 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ysr is ON HOLD	+	The entry 7ysr is ON HOLD until 2024-08-12

	Authors: Zhou, J., Wang, H.-W.		Authors: Zhou, J., Wang, H.-W.

OCA: Protected "7ysr" [edit=sysop:move=sysop]

2022-08-25T03:35:59Z

Protected "7ysr" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:35, 25 August 2022

OCA: New page: '''Unreleased structure''' The entry 7ysr is ON HOLD Authors: Zhou, J., Wang, H.-W. Description: GTPgammaS MT decorated with kinesin Category: Unreleased Structures [[Category: Wan...

2022-08-25T03:35:58Z

New page: '''Unreleased structure''' The entry 7ysr is ON HOLD Authors: Zhou, J., Wang, H.-W. Description: GTPgammaS MT decorated with kinesin Category: Unreleased Structures [[Category: Wan...

New page

'''Unreleased structure'''

The entry 7ysr is ON HOLD

Authors: Zhou, J., Wang, H.-W.

Description: GTPgammaS MT decorated with kinesin
[[Category: Unreleased Structures]]
[[Category: Wang, H.-W]]
[[Category: Zhou, J]]

7ysr - Revision history

OCA at 14:03, 18 October 2023

OCA at 08:49, 2 August 2023

OCA at 06:25, 21 September 2022

OCA: Protected "7ysr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ysr is ON HOLD Authors: Zhou, J., Wang, H.-W. Description: GTPgammaS MT decorated with kinesin Category: Unreleased Structures [[Category: Wan...