OCA at 09:05, 30 August 2023

2023-08-30T09:05:27Z

←Older revision		Revision as of 09:05, 30 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7yw0 is ~~ON HOLD until Paper~~ Publication	+	==Bacteroides fragilis Hcp5==
		+	<StructureSection load='7yw0' size='340' side='right'caption='[[7yw0]], [[Resolution\|resolution]] 1.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7yw0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_fragilis Bacteroides fragilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YW0 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.98Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yw0 OCA], [https://pdbe.org/7yw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yw0 RCSB], [https://www.ebi.ac.uk/pdbsum/7yw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yw0 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A081TQ32_BACFG A0A081TQ32_BACFG]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type VI secretion system is widely used in Gram-negative bacteria for injecting toxic effectors into neighboring prokaryotic or eukaryotic cells. Various effectors can be loaded onto the T6SS delivery tube via its core components: Hcp, VgrG, or PAAR. Here, we report 2.8-A resolution cryo-EM structure of intact T6SS Hcp5-VgrG-PAAR cargo delivery system and crystal structure of unbound Hcp5 from B. fragilis NCTC 9343. Loading of Hcp5 hexameric ring onto VgrG causes expansion of its inner cavity and external surface, explaining how structural changes could be propagated to regulate co-polymerization and surrounding contractile sheath. High-affinity binding between Hcp and VgrG causes entropically unfavorable structuring of long loops. Furthermore, interactions between VgrG trimer and Hcp hexamer are asymmetric, with three of the six Hcp monomers exhibiting a major loop flip. Our study provides insights into the assembly, loading, and firing of T6SS nanomachine that contributes to bacterial inter-species competition and host interactions.

-	~~Authors: Wen, Y~~., He, W., Bai, Y.	+	Structure and assembly of type VI secretion system cargo delivery vehicle.,He W, Wu K, Ouyang Z, Bai Y, Luo W, Wu D, An H, Guo Y, Jiao M, Qin Q, Zhang J, Wu Y, She J, Hwang PM, Zheng F, Zhu L, Wen Y Cell Rep. 2023 Jul 25;42(7):112781. doi: 10.1016/j.celrep.2023.112781. Epub 2023 , Jul 7. PMID:37421630<ref>PMID:37421630</ref>

-	~~Description~~: Bacteroides fragilis ~~Hcp5~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Bai, Y]]	+	<div class="pdbe-citations 7yw0" style="background-color:#fffaf0;"></div>
-	[[Category: He, W]]	+	== References ==
-	[[Category: Wen, Y]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacteroides fragilis]]
		+	[[Category: Large Structures]]
		+	[[Category: Bai Y]]
		+	[[Category: He W]]
		+	[[Category: Wen Y]]

OCA at 04:29, 28 September 2022

2022-09-28T04:29:25Z

←Older revision		Revision as of 04:29, 28 September 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yw0 is ON HOLD	+	The entry 7yw0 is ON HOLD until Paper Publication

	Authors: Wen, Y., He, W., Bai, Y.		Authors: Wen, Y., He, W., Bai, Y.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Bai, Y]]		[[Category: Bai, Y]]
-	[[Category: Wen, Y]]
	[[Category: He, W]]		[[Category: He, W]]
		+	[[Category: Wen, Y]]

OCA: Protected "7yw0" [edit=sysop:move=sysop]

2022-08-31T05:20:31Z

Protected "7yw0" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 31 August 2022

OCA: New page: '''Unreleased structure''' The entry 7yw0 is ON HOLD Authors: Wen, Y., He, W., Bai, Y. Description: Bacteroides fragilis Hcp5 Category: Unreleased Structures Category: Bai, Y [...

2022-08-31T05:20:30Z

New page: '''Unreleased structure''' The entry 7yw0 is ON HOLD Authors: Wen, Y., He, W., Bai, Y. Description: Bacteroides fragilis Hcp5 Category: Unreleased Structures Category: Bai, Y [...

New page

'''Unreleased structure'''

The entry 7yw0 is ON HOLD

Authors: Wen, Y., He, W., Bai, Y.

Description: Bacteroides fragilis Hcp5
[[Category: Unreleased Structures]]
[[Category: Bai, Y]]
[[Category: Wen, Y]]
[[Category: He, W]]

7yw0 - Revision history

OCA at 09:05, 30 August 2023

OCA at 04:29, 28 September 2022

OCA: Protected "7yw0" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7yw0 is ON HOLD Authors: Wen, Y., He, W., Bai, Y. Description: Bacteroides fragilis Hcp5 Category: Unreleased Structures Category: Bai, Y [...