7z1j - Revision history

OCA at 13:28, 1 February 2024

2024-02-01T13:28:23Z

←Older revision		Revision as of 13:28, 1 February 2024
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	<StructureSection load='7z1j' size='340' side='right'caption='[[7z1j]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='7z1j' size='340' side='right'caption='[[7z1j]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7z1j]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z1J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7z1j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z1J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dkl\|1dkl]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z1j OCA], [https://pdbe.org/7z1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z1j RCSB], [https://www.ebi.ac.uk/pdbsum/7z1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z1j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z1j OCA], [https://pdbe.org/7z1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z1j RCSB], [https://www.ebi.ac.uk/pdbsum/7z1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z1j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PPA_ECOLI PPA_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7z1j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7z1j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]
		+	*[[Phytase 3D structures\|Phytase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acquistapace~~, I M~~]]	+	[[Category: Acquistapace IM]]
-	[[Category: Brearley~~, C A~~]]	+	[[Category: Brearley CA]]
-	[[Category: Hemmings~~, A M]]~~	+	[[Category: Hemmings AM]]
-	~~[[Category: Histidine acid phosphatase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phytase~~]]	+

OCA at 07:36, 29 June 2022

2022-06-29T07:36:17Z

←Older revision		Revision as of 07:36, 29 June 2022
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	==Escherichia coli periplasmic phytase AppA, complex with phosphate==		==Escherichia coli periplasmic phytase AppA, complex with phosphate==
-	<StructureSection load='7z1j' size='340' side='right'caption='[[7z1j]]' scene=''>	+	<StructureSection load='7z1j' size='340' side='right'caption='[[7z1j]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z1J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7z1j]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z1J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z1j OCA], [https://pdbe.org/7z1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z1j RCSB], [https://www.ebi.ac.uk/pdbsum/7z1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z1j ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dkl\|1dkl]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z1j OCA], [https://pdbe.org/7z1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z1j RCSB], [https://www.ebi.ac.uk/pdbsum/7z1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z1j ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	AppA, the Escherichia coli periplasmic phytase of clade 2 of the histidine phosphatase (HP2) family, has been well-characterized and successfully engineered for use as an animal feed supplement. AppA is a 1D-6-phytase and highly stereospecific but transiently accumulates 1D-myo-Ins(2,3,4,5)P4 and other lower phosphorylated intermediates. If this bottleneck in liberation of orthophosphate is to be obviated through protein engineering, an explanation of its rather rigid preference for the initial site and subsequent cleavage of phytic acid is required. To help explain this behaviour, the role of the catalytic proton donor residue in determining AppA stereospecificity was investigated. Four variants were generated by site-directed mutagenesis of the active site HDT amino acid sequence motif containing the catalytic proton donor, D304. The identity and position of the prospective proton donor residue was found to strongly influence stereospecificity. While the wild-type enzyme has a strong preference for 1D-6-phytase activity, a marked reduction in stereospecificity was observed for a D304E variant, while a proton donor-less mutant (D304A) displayed exclusive 1D-1/3-phytase activity. High-resolution X-ray crystal structures of complexes of the mutants with a non-hydrolysable substrate analogue inhibitor point to a crucial role played by D304 in stereospecificity by influencing the size and polarity of specificity pockets A and B. Taken together, these results provide the first evidence for the involvement of the proton donor residue in determining the stereospecificity of HP2 phytases and prepares the ground for structure-informed engineering studies targeting the production of animal feed enzymes capable of the efficient and complete dephosphorylation of dietary phytic acid.
		+
		+	Insights to the Structural Basis for the Stereospecificity of the Escherichia coli Phytase, AppA.,Acquistapace IM, Thompson EJ, Kuhn I, Bedford MR, Brearley CA, Hemmings AM Int J Mol Sci. 2022 Jun 6;23(11). pii: ijms23116346. doi: 10.3390/ijms23116346. PMID:35683026<ref>PMID:35683026</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7z1j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acquistapace IM]]	+	[[Category: Acquistapace, I M]]
-	[[Category: Brearley CA]]	+	[[Category: Brearley, C A]]
-	[[Category: Hemmings AM]]	+	[[Category: Hemmings, A M]]
		+	[[Category: Histidine acid phosphatase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phytase]]

OCA at 06:19, 16 March 2022

2022-03-16T06:19:05Z

←Older revision		Revision as of 06:19, 16 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7z1j ~~is ON HOLD until Paper Publication~~	+	==Escherichia coli periplasmic phytase AppA, complex with phosphate==
-		+	<StructureSection load='7z1j' size='340' side='right'caption='[[7z1j]]' scene=''>
-	~~Authors~~: ~~Acquistapace~~, I.M., ~~Brearley~~, C.A., ~~Hemmings~~, A.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z1J FirstGlance]. <br>
-	~~Description: Escherichia coli periplasmic phytase AppA, complex with phosphate~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z1j OCA], [https://pdbe.org/7z1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z1j RCSB], [https://www.ebi.ac.uk/pdbsum/7z1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z1j ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Brearley, C.A~~]]	+	__TOC__
-	[[Category: ~~Acquistapace, I.M~~]]	+	</StructureSection>
-	[[Category: Hemmings~~, A.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Acquistapace IM]]
		+	[[Category: Brearley CA]]
		+	[[Category: Hemmings AM]]

OCA at 05:09, 9 March 2022

2022-03-09T05:09:37Z

←Older revision		Revision as of 05:09, 9 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7z1j is ON HOLD	+	The entry 7z1j is ON HOLD until Paper Publication

	Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M.		Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M.

OCA: Protected "7z1j" [edit=sysop:move=sysop]

2022-03-02T05:24:05Z

Protected "7z1j" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:24, 2 March 2022

OCA: New page: '''Unreleased structure''' The entry 7z1j is ON HOLD Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M. Description: Escherichia coli periplasmic phytase AppA, complex with ph...

2022-03-02T05:24:04Z

New page: '''Unreleased structure''' The entry 7z1j is ON HOLD Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M. Description: Escherichia coli periplasmic phytase AppA, complex with ph...

New page

'''Unreleased structure'''

The entry 7z1j is ON HOLD

Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M.

Description: Escherichia coli periplasmic phytase AppA, complex with phosphate
[[Category: Unreleased Structures]]
[[Category: Brearley, C.A]]
[[Category: Acquistapace, I.M]]
[[Category: Hemmings, A.M]]