7z2w - Revision history

OCA at 13:28, 1 February 2024

2024-02-01T13:28:39Z

←Older revision		Revision as of 13:28, 1 February 2024
Line 3:		Line 3:
	<StructureSection load='7z2w' size='340' side='right'caption='[[7z2w]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='7z2w' size='340' side='right'caption='[[7z2w]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7z2w]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7z2w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z2W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.42Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7z2s\|7z2s]], [[7z2t\|7z2t]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z2w OCA], [https://pdbe.org/7z2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z2w RCSB], [https://www.ebi.ac.uk/pdbsum/7z2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z2w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z2w OCA], [https://pdbe.org/7z2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z2w RCSB], [https://www.ebi.ac.uk/pdbsum/7z2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z2w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PPA_ECOLI PPA_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7z2w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7z2w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]
		+	*[[Phytase 3D structures\|Phytase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acquistapace~~, I M~~]]	+	[[Category: Acquistapace IM]]
-	[[Category: Brearley~~, C A~~]]	+	[[Category: Brearley CA]]
-	[[Category: Hemmings~~, A M]]~~	+	[[Category: Hemmings AM]]
-	~~[[Category: Histidine acid phosphatase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phytase~~]]	+

OCA at 07:36, 29 June 2022

2022-06-29T07:36:17Z

←Older revision		Revision as of 07:36, 29 June 2022
Line 1:		Line 1:

	==Escherichia coli periplasmic phytase AppA D304A,T305E mutant, complex with myo-inositol hexakissulfate==		==Escherichia coli periplasmic phytase AppA D304A,T305E mutant, complex with myo-inositol hexakissulfate==
-	<StructureSection load='7z2w' size='340' side='right'caption='[[7z2w]]' scene=''>	+	<StructureSection load='7z2w' size='340' side='right'caption='[[7z2w]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7z2w]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z2W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z2w OCA], [https://pdbe.org/7z2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z2w RCSB], [https://www.ebi.ac.uk/pdbsum/7z2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z2w ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7z2s\|7z2s]], [[7z2t\|7z2t]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z2w OCA], [https://pdbe.org/7z2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z2w RCSB], [https://www.ebi.ac.uk/pdbsum/7z2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z2w ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	AppA, the Escherichia coli periplasmic phytase of clade 2 of the histidine phosphatase (HP2) family, has been well-characterized and successfully engineered for use as an animal feed supplement. AppA is a 1D-6-phytase and highly stereospecific but transiently accumulates 1D-myo-Ins(2,3,4,5)P4 and other lower phosphorylated intermediates. If this bottleneck in liberation of orthophosphate is to be obviated through protein engineering, an explanation of its rather rigid preference for the initial site and subsequent cleavage of phytic acid is required. To help explain this behaviour, the role of the catalytic proton donor residue in determining AppA stereospecificity was investigated. Four variants were generated by site-directed mutagenesis of the active site HDT amino acid sequence motif containing the catalytic proton donor, D304. The identity and position of the prospective proton donor residue was found to strongly influence stereospecificity. While the wild-type enzyme has a strong preference for 1D-6-phytase activity, a marked reduction in stereospecificity was observed for a D304E variant, while a proton donor-less mutant (D304A) displayed exclusive 1D-1/3-phytase activity. High-resolution X-ray crystal structures of complexes of the mutants with a non-hydrolysable substrate analogue inhibitor point to a crucial role played by D304 in stereospecificity by influencing the size and polarity of specificity pockets A and B. Taken together, these results provide the first evidence for the involvement of the proton donor residue in determining the stereospecificity of HP2 phytases and prepares the ground for structure-informed engineering studies targeting the production of animal feed enzymes capable of the efficient and complete dephosphorylation of dietary phytic acid.
		+
		+	Insights to the Structural Basis for the Stereospecificity of the Escherichia coli Phytase, AppA.,Acquistapace IM, Thompson EJ, Kuhn I, Bedford MR, Brearley CA, Hemmings AM Int J Mol Sci. 2022 Jun 6;23(11). pii: ijms23116346. doi: 10.3390/ijms23116346. PMID:35683026<ref>PMID:35683026</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7z2w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acquistapace IM]]	+	[[Category: Acquistapace, I M]]
-	[[Category: Brearley CA]]	+	[[Category: Brearley, C A]]
-	[[Category: Hemmings AM]]	+	[[Category: Hemmings, A M]]
		+	[[Category: Histidine acid phosphatase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phytase]]

OCA at 06:19, 16 March 2022

2022-03-16T06:19:09Z

←Older revision		Revision as of 06:19, 16 March 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7z2w ~~is ON HOLD~~	+	==Escherichia coli periplasmic phytase AppA D304A,T305E mutant, complex with myo-inositol hexakissulfate==
-		+	<StructureSection load='7z2w' size='340' side='right'caption='[[7z2w]]' scene=''>
-	~~Authors~~: ~~Acquistapace~~, I.M., ~~Brearley~~, C.A., ~~Hemmings~~, A.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z2W FirstGlance]. <br>
-	~~Description: Escherichia coli periplasmic phytase AppA D304A,T305E mutant, complex with myo-inositol hexakissulfate~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z2w OCA], [https://pdbe.org/7z2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z2w RCSB], [https://www.ebi.ac.uk/pdbsum/7z2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z2w ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Brearley, C.A~~]]	+	__TOC__
-	[[Category: ~~Acquistapace, I.M~~]]	+	</StructureSection>
-	[[Category: Hemmings~~, A.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Acquistapace IM]]
		+	[[Category: Brearley CA]]
		+	[[Category: Hemmings AM]]

OCA: Protected "7z2w" [edit=sysop:move=sysop]

2022-03-09T05:11:28Z

Protected "7z2w" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 9 March 2022

OCA: New page: '''Unreleased structure''' The entry 7z2w is ON HOLD Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M. Description: Escherichia coli periplasmic phytase AppA D304A,T305E muta...

2022-03-09T05:11:27Z

New page: '''Unreleased structure''' The entry 7z2w is ON HOLD Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M. Description: Escherichia coli periplasmic phytase AppA D304A,T305E muta...

New page

'''Unreleased structure'''

The entry 7z2w is ON HOLD

Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M.

Description: Escherichia coli periplasmic phytase AppA D304A,T305E mutant, complex with myo-inositol hexakissulfate
[[Category: Unreleased Structures]]
[[Category: Brearley, C.A]]
[[Category: Acquistapace, I.M]]
[[Category: Hemmings, A.M]]