7zc2 - Revision history

OCA at 06:50, 8 September 2022

2022-09-08T06:50:47Z

←Older revision		Revision as of 06:50, 8 September 2022
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	<StructureSection load='7zc2' size='340' side='right'caption='[[7zc2]], [[Resolution\|resolution]] 2.72Å' scene=''>		<StructureSection load='7zc2' size='340' side='right'caption='[[7zc2]], [[Resolution\|resolution]] 2.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7zc2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZC2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7zc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZC2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zc2 OCA], [https://pdbe.org/7zc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zc2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zc2 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zc2 OCA], [https://pdbe.org/7zc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zc2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zc2 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/W8TWZ0_ECOLX W8TWZ0_ECOLX]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Finocchio, G]]	+	[[Category: Finocchio G]]
-	[[Category: Killer, M]]	+	[[Category: Killer M]]
-	[[Category: Loew, C]]	+	[[Category: Loew C]]
-	[[Category: Pardon, E]]	+	[[Category: Pardon E]]
-	[[Category: Steyaert, J]]	+	[[Category: Steyaert J]]
-	~~[[Category: Dtpc]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Mf]]~~	+
-	~~[[Category: Peptide transporter]]~~	+
-	~~[[Category: Pot]]~~	+
-	~~[[Category: Proton coupled oligopeptide transporter]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 05:15, 10 August 2022

2022-08-10T05:15:12Z

←Older revision		Revision as of 05:15, 10 August 2022
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	==Dipeptide and tripeptide Permease C (DtpC)==		==Dipeptide and tripeptide Permease C (DtpC)==
-	<StructureSection load='7zc2' size='340' side='right'caption='[[7zc2]]' scene=''>	+	<StructureSection load='7zc2' size='340' side='right'caption='[[7zc2]], [[Resolution\|resolution]] 2.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZC2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7zc2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZC2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zc2 OCA], [https://pdbe.org/7zc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zc2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zc2 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zc2 OCA], [https://pdbe.org/7zc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zc2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zc2 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proton-coupled Oligopeptide Transporters (POTs) of the Major Facilitator Superfamily (MFS) mediate the uptake of short di- and tripeptides in all phyla of life. POTs are thought to constitute the most promiscuous class of MFS transporters, with the potential to transport more than 8400 unique substrates. Over the past two decades, transport assays and biophysical studies have shown that various orthologues and paralogues display differences in substrate selectivity. The E. coli genome codes for four different POTs, known as Di- and tripeptide permeases A-D (DtpA-D). DtpC was shown previously to favor positively charged peptides as substrates. In this study, we describe, how we determined the structure of the 53 kDa DtpC by cryogenic electron microscopy (cryo-EM), and provide structural insights into the ligand specificity of this atypical POT. We collected and analyzed data on the transporter fused to split superfolder GFP (split sfGFP), in complex with a 52 kDa Pro-macrobody and with a 13 kDa nanobody. The latter sample was more stable, rigid and a significant fraction dimeric, allowing us to reconstruct a 3D volume of DtpC at a resolution of 2.7 A. This work provides a molecular explanation for the selectivity of DtpC, and highlights the value of small and rigid fiducial markers such as nanobodies for structure determination of low molecular weight integral membrane proteins lacking soluble domains.
		+
		+	Cryo-EM Structure of an Atypical Proton-Coupled Peptide Transporter: Di- and Tripeptide Permease C.,Killer M, Finocchio G, Mertens HDT, Svergun DI, Pardon E, Steyaert J, Low C Front Mol Biosci. 2022 Jul 11;9:917725. doi: 10.3389/fmolb.2022.917725., eCollection 2022. PMID:35898305<ref>PMID:35898305</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7zc2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Finocchio G]]	+	[[Category: Finocchio, G]]
-	[[Category: Killer M]]	+	[[Category: Killer, M]]
-	[[Category: Loew C]]	+	[[Category: Loew, C]]
-	[[Category: Pardon E]]	+	[[Category: Pardon, E]]
-	[[Category: Steyaert J]]	+	[[Category: Steyaert, J]]
		+	[[Category: Dtpc]]
		+	[[Category: Membrane protein]]
		+	[[Category: Mf]]
		+	[[Category: Peptide transporter]]
		+	[[Category: Pot]]
		+	[[Category: Proton coupled oligopeptide transporter]]
		+	[[Category: Transport protein]]

OCA at 16:16, 6 July 2022

2022-07-06T16:16:20Z

←Older revision		Revision as of 16:16, 6 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7zc2 is ~~ON HOLD until Paper Publication~~	+	==Dipeptide and tripeptide Permease C (DtpC)==
-		+	<StructureSection load='7zc2' size='340' side='right'caption='[[7zc2]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZC2 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zc2 OCA], [https://pdbe.org/7zc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zc2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zc2 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Finocchio G]]
		+	[[Category: Killer M]]
		+	[[Category: Loew C]]
		+	[[Category: Pardon E]]
		+	[[Category: Steyaert J]]

OCA at 05:01, 12 May 2022

2022-05-12T05:01:40Z

←Older revision		Revision as of 05:01, 12 May 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zc2 is ON HOLD	+	The entry 7zc2 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7zc2" [edit=sysop:move=sysop]

2022-04-06T09:21:52Z

Protected "7zc2" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:21, 6 April 2022

OCA: New page: '''Unreleased structure''' The entry 7zc2 is ON HOLD Authors: Description: Category: Unreleased Structures

2022-04-06T09:21:51Z

New page: '''Unreleased structure''' The entry 7zc2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7zc2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]