OCA at 10:07, 14 December 2022

2022-12-14T10:07:33Z

←Older revision		Revision as of 10:07, 14 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7zr0 is ~~ON HOLD until Paper~~ Publication	+	==CryoEM structure of HSP90-CDC37-BRAF(V600E) complex==
		+	<StructureSection load='7zr0' size='340' side='right'caption='[[7zr0]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7zr0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZR0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zr0 OCA], [https://pdbe.org/7zr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zr0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zr0 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HS90B_HUMAN HS90B_HUMAN] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.<ref>PMID:16478993</ref> <ref>PMID:19696785</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Activation of client protein kinases by the HSP90 molecular chaperone system is affected by phosphorylation at multiple sites on HSP90, the kinase-specific co-chaperone CDC37, and the kinase client itself. Removal of regulatory phosphorylation from client kinases and their release from the HSP90-CDC37 system depends on the Ser/Thr phosphatase PP5, which associates with HSP90 via its N-terminal TPR domain. Here, we present the cryoEM structure of the oncogenic protein kinase client BRAF(V600E) bound to HSP90-CDC37, showing how the V600E mutation favours BRAF association with HSP90-CDC37. Structures of HSP90-CDC37-BRAF(V600E) complexes with PP5 in autoinhibited and activated conformations, together with proteomic analysis of its phosphatase activity on BRAF(V600E) and CRAF, reveal how PP5 is activated by recruitment to HSP90 complexes. PP5 comprehensively dephosphorylates client proteins, removing interaction sites for regulatory partners such as 14-3-3 proteins and thus performing a 'factory reset' of the kinase prior to release.

-	~~Authors~~:	+	HSP90-CDC37-PP5 forms a structural platform for kinase dephosphorylation.,Oberoi J, Guiu XA, Outwin EA, Schellenberger P, Roumeliotis TI, Choudhary JS, Pearl LH Nat Commun. 2022 Nov 29;13(1):7343. doi: 10.1038/s41467-022-35143-2. PMID:36446791<ref>PMID:36446791</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7zr0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Oberoi J]]
		+	[[Category: Pearl LH]]

OCA at 06:14, 29 June 2022

2022-06-29T06:14:49Z

←Older revision		Revision as of 06:14, 29 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zr0 is ON HOLD	+	The entry 7zr0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7zr0" [edit=sysop:move=sysop]

2022-05-12T05:08:42Z

Protected "7zr0" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:08, 12 May 2022

OCA: New page: '''Unreleased structure''' The entry 7zr0 is ON HOLD Authors: Description: Category: Unreleased Structures

2022-05-12T05:08:41Z

New page: '''Unreleased structure''' The entry 7zr0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7zr0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7zr0 - Revision history

OCA at 10:07, 14 December 2022

OCA at 06:14, 29 June 2022

OCA: Protected "7zr0" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7zr0 is ON HOLD Authors: Description: Category: Unreleased Structures