8a2u - Revision history

OCA at 20:29, 16 November 2022

2022-11-16T20:29:15Z

←Older revision		Revision as of 20:29, 16 November 2022
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	==Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3- nucleotide state.==		==Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3- nucleotide state.==
-	<StructureSection load='8a2u' size='340' side='right'caption='[[8a2u]]' scene=''>	+	<StructureSection load='8a2u' size='340' side='right'caption='[[8a2u]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[8a2u]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A2U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a2u OCA], [https://pdbe.org/8a2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a2u RCSB], [https://www.ebi.ac.uk/pdbsum/8a2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a2u ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a2u OCA], [https://pdbe.org/8a2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a2u RCSB], [https://www.ebi.ac.uk/pdbsum/8a2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a2u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dynamic turnover of actin filaments (F-actin) controls cellular motility in eukaryotes and is coupled to changes in the F-actin nucleotide state<sup>1-3</sup>. It remains unclear how F-actin hydrolyses ATP and subsequently undergoes subtle conformational rearrangements that ultimately lead to filament depolymerization by actin-binding proteins. Here we present cryo-electron microscopy structures of F-actin in all nucleotide states, polymerized in the presence of Mg<sup>2+</sup> or Ca<sup>2+</sup> at approximately 2.2 A resolution. The structures show that actin polymerization induces the relocation of water molecules in the nucleotide-binding pocket, activating one of them for the nucleophilic attack of ATP. Unexpectedly, the back door for the subsequent release of inorganic phosphate (P<sub>i</sub>) is closed in all structures, indicating that P<sub>i</sub> release occurs transiently. The small changes in the nucleotide-binding pocket after ATP hydrolysis and P<sub>i</sub> release are sensed by a key amino acid, amplified and transmitted to the filament periphery. Furthermore, differences in the positions of water molecules in the nucleotide-binding pocket explain why Ca<sup>2+</sup>-actin shows slower polymerization rates than Mg<sup>2+</sup>-actin. Our work elucidates the solvent-driven rearrangements that govern actin filament assembly and aging and lays the foundation for the rational design of drugs and small molecules for imaging and therapeutic applications.
		+
		+	Structural basis of actin filament assembly and aging.,Oosterheert W, Klink BU, Belyy A, Pospich S, Raunser S Nature. 2022 Nov;611(7935):374-379. doi: 10.1038/s41586-022-05241-8. Epub 2022, Oct 26. PMID:36289337<ref>PMID:36289337</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8a2u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Oryctolagus cuniculus]]
	[[Category: Belyy A]]		[[Category: Belyy A]]
	[[Category: Klink BU]]		[[Category: Klink BU]]

OCA at 05:03, 10 August 2022

2022-08-10T05:03:35Z

←Older revision		Revision as of 05:03, 10 August 2022
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-	'''Unreleased structure'''

-	~~The entry 8a2u is ON HOLD until Paper Publication~~	+	==Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3- nucleotide state.==
-		+	<StructureSection load='8a2u' size='340' side='right'caption='[[8a2u]]' scene=''>
-	~~Authors: Oosterheert, W., Klink, B.U., Belyy, A., Pospich, S., Raunser, S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A2U FirstGlance]. <br>
-	~~Description:~~ Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3-nucleotide state.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a2u OCA], [https://pdbe.org/8a2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a2u RCSB], [https://www.ebi.ac.uk/pdbsum/8a2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a2u ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Pospich, S~~]]	+	__TOC__
-	[[Category: ~~Oosterheert, W~~]]	+	</StructureSection>
-	[[Category: ~~Klink, B.U~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Belyy, A~~]]	+	[[Category: Belyy A]]
-	[[Category: Raunser, S]]	+	[[Category: Klink BU]]
		+	[[Category: Oosterheert W]]
		+	[[Category: Pospich S]]
		+	[[Category: Raunser S]]

OCA at 04:05, 13 July 2022

2022-07-13T04:05:51Z

←Older revision		Revision as of 04:05, 13 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8a2u is ON HOLD	+	The entry 8a2u is ON HOLD until Paper Publication

	Authors: Oosterheert, W., Klink, B.U., Belyy, A., Pospich, S., Raunser, S.		Authors: Oosterheert, W., Klink, B.U., Belyy, A., Pospich, S., Raunser, S.

OCA: Protected "8a2u" [edit=sysop:move=sysop]

2022-06-15T04:06:10Z

Protected "8a2u" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:06, 15 June 2022

OCA: New page: '''Unreleased structure''' The entry 8a2u is ON HOLD Authors: Oosterheert, W., Klink, B.U., Belyy, A., Pospich, S., Raunser, S. Description: Cryo-EM structure of F-actin in the Ca2+-AD...

2022-06-15T04:06:09Z

New page: '''Unreleased structure''' The entry 8a2u is ON HOLD Authors: Oosterheert, W., Klink, B.U., Belyy, A., Pospich, S., Raunser, S. Description: Cryo-EM structure of F-actin in the Ca2+-AD...

New page

'''Unreleased structure'''

The entry 8a2u is ON HOLD

Authors: Oosterheert, W., Klink, B.U., Belyy, A., Pospich, S., Raunser, S.

Description: Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3-nucleotide state.
[[Category: Unreleased Structures]]
[[Category: Pospich, S]]
[[Category: Oosterheert, W]]
[[Category: Klink, B.U]]
[[Category: Belyy, A]]
[[Category: Raunser, S]]