OCA at 06:08, 26 October 2022

2022-10-26T06:08:25Z

←Older revision		Revision as of 06:08, 26 October 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8a8u is ~~ON HOLD until Paper~~ Publication	+	==Mycobacterium tuberculosis ClpC1 hexamer structure==
		+	<StructureSection load='8a8u' size='340' side='right'caption='[[8a8u]], [[Resolution\|resolution]] 3.62Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8a8u]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A8U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a8u OCA], [https://pdbe.org/8a8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a8u RCSB], [https://www.ebi.ac.uk/pdbsum/8a8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a8u ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CLPC1_MYCTU CLPC1_MYCTU] ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity). Degrades anti-sigma-E factor RseA in the presence of ClpP2.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The unfoldase ClpC1 is one of the most exciting drug targets against tuberculosis. This AAA+ unfoldase works in cooperation with the ClpP1P2 protease and is the target of at least four natural product antibiotics: cyclomarin, ecumicin, lassomycin and rufomycin. Although these molecules are promising starting points for drug development, their mechanisms of action remain largely unknown. Taking advantage of a middle domain mutant, we determined the first structure of Mycobacterium tuberculosis ClpC1 in its apo, cyclomarin and ecumicin bound states via cryo-electron microscopy. The obtained structure displays features observed in other members of the AAA+ family and provides a map for further drug development. While the apo and cyclomarin bound structures are indistinguishable and have N-terminal domains (NTDs) that are invisible in their respective EM maps, around half of the ecumicin-bound ClpC1 particles display three of their six NTDs in an extended conformation. Our structural observations suggest a mechanism where ecumicin functions by mimicking substrate binding, leading to ATPase activation and changes in protein degradation profile.

-	~~Authors~~:	+	Structure of the drug target ClpC1 unfoldase in action provides insights on antibiotic mechanism of action.,Weinhaupl K, Gragera M, Bueno-Carrasco MT, Arranz R, Krandor O, Akopian T, Soares R, Rubin E, Felix J, Fraga H J Biol Chem. 2022 Oct 5:102553. doi: 10.1016/j.jbc.2022.102553. PMID:36208775<ref>PMID:36208775</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8a8u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Bueno T]]
		+	[[Category: Felix J]]
		+	[[Category: Fraga H]]
		+	[[Category: Gragera M]]
		+	[[Category: Weinhaeupl K]]

OCA at 03:45, 3 August 2022

2022-08-03T03:45:45Z

←Older revision		Revision as of 03:45, 3 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8a8u is ON HOLD	+	The entry 8a8u is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8a8u" [edit=sysop:move=sysop]

2022-06-29T06:24:14Z

Protected "8a8u" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:24, 29 June 2022

OCA: New page: '''Unreleased structure''' The entry 8a8u is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-29T06:24:13Z

New page: '''Unreleased structure''' The entry 8a8u is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8a8u is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8a8u - Revision history

OCA at 06:08, 26 October 2022

OCA at 03:45, 3 August 2022

OCA: Protected "8a8u" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8a8u is ON HOLD Authors: Description: Category: Unreleased Structures