8aws - Revision history

OCA at 08:14, 7 February 2024

2024-02-07T08:14:34Z

←Older revision		Revision as of 08:14, 7 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8aws]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AWS FirstGlance]. <br>		<table><tr><td colspan='2'>[[8aws]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AWS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aws OCA], [https://pdbe.org/8aws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aws RCSB], [https://www.ebi.ac.uk/pdbsum/8aws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aws ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aws OCA], [https://pdbe.org/8aws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aws RCSB], [https://www.ebi.ac.uk/pdbsum/8aws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aws ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism.		[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals are kept in a humidity and temperature-controlled environment, then reactions are initiated via the liquid application method (LAMA) and plunging into liquid nitrogen is initiated after an electronically set delay time to cryo-trap intermediate states. High-magnification images are automatically recorded before and after droplet deposition, prior to plunging. The SPINE-standard sample holder is directly plunged into a storage puck, enabling compatibility with high-throughput infrastructure. Here we demonstrate binding of glucose and 2,3-butanediol in microcrystals of xylose isomerase, and of avibactam and ampicillin in microcrystals of the extended spectrum beta-lactamase CTX-M-14. We also trap reaction intermediates and conformational changes in macroscopic crystals of tryptophan synthase to demonstrate that the spitrobot enables insight into catalytic events.
-
-	Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography.,Mehrabi P, Sung S, von Stetten D, Prester A, Hatton CE, Kleine-Dopke S, Berkes A, Gore G, Leimkohl JP, Schikora H, Kollewe M, Rohde H, Wilmanns M, Tellkamp F, Schulz EC Nat Commun. 2023 Apr 25;14(1):2365. doi: 10.1038/s41467-023-37834-w. PMID:37185266<ref>PMID:37185266</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8aws" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:01, 25 May 2023

2023-05-25T04:01:20Z

←Older revision		Revision as of 04:01, 25 May 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8aws is ~~ON HOLD until Paper~~ Publication	+	==Millisecond cryo-trapping by the spitrobot crystal plunger, Xylose Isomerase with Glucose at 50ms==
		+	<StructureSection load='8aws' size='340' side='right'caption='[[8aws]], [[Resolution\|resolution]] 2.26Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8aws]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AWS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aws OCA], [https://pdbe.org/8aws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aws RCSB], [https://www.ebi.ac.uk/pdbsum/8aws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aws ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals are kept in a humidity and temperature-controlled environment, then reactions are initiated via the liquid application method (LAMA) and plunging into liquid nitrogen is initiated after an electronically set delay time to cryo-trap intermediate states. High-magnification images are automatically recorded before and after droplet deposition, prior to plunging. The SPINE-standard sample holder is directly plunged into a storage puck, enabling compatibility with high-throughput infrastructure. Here we demonstrate binding of glucose and 2,3-butanediol in microcrystals of xylose isomerase, and of avibactam and ampicillin in microcrystals of the extended spectrum beta-lactamase CTX-M-14. We also trap reaction intermediates and conformational changes in macroscopic crystals of tryptophan synthase to demonstrate that the spitrobot enables insight into catalytic events.

-	~~Authors: Mehrabi~~, P., Sung, S., von Stetten, D., Prester, A., Hatton~~, C.E.~~, Kleine-~~Doepke,~~ S., Berkes, A., Gore, G., Leimkohl~~, J.P.~~, Schikora, H., Kollewe, M., Rohde, H., Wilmanns, M., Tellkamp, F., Schulz~~, E~~.C.	+	Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography.,Mehrabi P, Sung S, von Stetten D, Prester A, Hatton CE, Kleine-Dopke S, Berkes A, Gore G, Leimkohl JP, Schikora H, Kollewe M, Rohde H, Wilmanns M, Tellkamp F, Schulz EC Nat Commun. 2023 Apr 25;14(1):2365. doi: 10.1038/s41467-023-37834-w. PMID:37185266<ref>PMID:37185266</ref>

-	~~Description: Millisecond cryo-trapping by the spitrobot crystal plunger~~, ~~Xylose Isomerase with Glucose at 50ms~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Mehrabi, P~~]]	+	<div class="pdbe-citations 8aws" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Gore, G~~]]	+	== References ==
-	[[Category: ~~Sung, S~~]]	+	<references/>
-	[[Category: ~~Rohde, H~~]]	+	__TOC__
-	[[Category: Kleine-Doepke, S]]	+	</StructureSection>
-	[[Category: ~~Hatton, C.E~~]]	+	[[Category: Large Structures]]
-	[[Category: Leimkohl~~, J.P~~]]	+	[[Category: Streptomyces rubiginosus]]
-	[[Category: ~~Wilmanns, M~~]]	+	[[Category: Berkes A]]
-	[[Category: Prester, A]]	+	[[Category: Gore G]]
-	[[Category: ~~Von Stetten, D~~]]	+	[[Category: Hatton CE]]
-	[[Category: ~~Tellkamp, F~~]]	+	[[Category: Kleine-Doepke S]]
-	[[Category: ~~Kollewe, M~~]]	+	[[Category: Kollewe M]]
-	[[Category: ~~Schikora, H~~]]	+	[[Category: Leimkohl JP]]
-	[[Category: ~~Berkes, A~~]]	+	[[Category: Mehrabi P]]
-	[[Category: ~~Schulz, E.C~~]]	+	[[Category: Prester A]]
		+	[[Category: Rohde H]]
		+	[[Category: Schikora H]]
		+	[[Category: Schulz EC]]
		+	[[Category: Sung S]]
		+	[[Category: Tellkamp F]]
		+	[[Category: Wilmanns M]]
		+	[[Category: Von Stetten D]]

OCA at 16:39, 19 October 2022

2022-10-19T16:39:38Z

←Older revision		Revision as of 16:39, 19 October 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8aws is ON HOLD	+	The entry 8aws is ON HOLD until Paper Publication

	Authors: Mehrabi, P., Sung, S., von Stetten, D., Prester, A., Hatton, C.E., Kleine-Doepke, S., Berkes, A., Gore, G., Leimkohl, J.P., Schikora, H., Kollewe, M., Rohde, H., Wilmanns, M., Tellkamp, F., Schulz, E.C.		Authors: Mehrabi, P., Sung, S., von Stetten, D., Prester, A., Hatton, C.E., Kleine-Doepke, S., Berkes, A., Gore, G., Leimkohl, J.P., Schikora, H., Kollewe, M., Rohde, H., Wilmanns, M., Tellkamp, F., Schulz, E.C.

OCA: Protected "8aws" [edit=sysop:move=sysop]

2022-09-07T15:26:28Z

Protected "8aws" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:26, 7 September 2022

OCA: New page: '''Unreleased structure''' The entry 8aws is ON HOLD Authors: Mehrabi, P., Sung, S., von Stetten, D., Prester, A., Hatton, C.E., Kleine-Doepke, S., Berkes, A., Gore, G., Leimkohl, J.P.,...

2022-09-07T15:26:28Z

New page: '''Unreleased structure''' The entry 8aws is ON HOLD Authors: Mehrabi, P., Sung, S., von Stetten, D., Prester, A., Hatton, C.E., Kleine-Doepke, S., Berkes, A., Gore, G., Leimkohl, J.P.,...

New page

'''Unreleased structure'''

The entry 8aws is ON HOLD

Authors: Mehrabi, P., Sung, S., von Stetten, D., Prester, A., Hatton, C.E., Kleine-Doepke, S., Berkes, A., Gore, G., Leimkohl, J.P., Schikora, H., Kollewe, M., Rohde, H., Wilmanns, M., Tellkamp, F., Schulz, E.C.

Description: Millisecond cryo-trapping by the spitrobot crystal plunger, Xylose Isomerase with Glucose at 50ms
[[Category: Unreleased Structures]]
[[Category: Mehrabi, P]]
[[Category: Gore, G]]
[[Category: Sung, S]]
[[Category: Rohde, H]]
[[Category: Kleine-Doepke, S]]
[[Category: Hatton, C.E]]
[[Category: Leimkohl, J.P]]
[[Category: Wilmanns, M]]
[[Category: Prester, A]]
[[Category: Von Stetten, D]]
[[Category: Tellkamp, F]]
[[Category: Kollewe, M]]
[[Category: Schikora, H]]
[[Category: Berkes, A]]
[[Category: Schulz, E.C]]