OCA at 06:47, 29 March 2023

2023-03-29T06:47:57Z

←Older revision		Revision as of 06:47, 29 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8axv is ~~ON HOLD~~	+	==Structure of an open form of CHIKV nsP1 capping pores==
		+	<StructureSection load='8axv' size='340' side='right'caption='[[8axv]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8axv]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Chikungunya_virus_strain_S27-African_prototype Chikungunya virus strain S27-African prototype]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AXV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MY6:2-AMINO-7-METHYL-1,7-DIHYDRO-6H-PURIN-6-ONE'>MY6</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8axv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8axv OCA], [https://pdbe.org/8axv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8axv RCSB], [https://www.ebi.ac.uk/pdbsum/8axv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8axv ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Alphaviruses are emerging positive-stranded RNA viruses which replicate and transcribe their genomes in membranous organelles formed in the cell cytoplasm. The nonstructural protein 1 (nsP1) is responsible for viral RNA capping and gates the replication organelles by assembling into monotopic membrane-associated dodecameric pores. The capping pathway is unique to Alphaviruses; beginning with the N(7) methylation of a guanosine triphosphate (GTP) molecule, followed by the covalent linkage of an m(7)GMP group to a conserved histidine in nsP1 and the transfer of this cap structure to a diphosphate RNA. Here, we provide structural snapshots of different stages of the reaction pathway showing how nsP1 pores recognize the substrates of the methyl-transfer reaction, GTP and S-adenosyl methionine (SAM), how the enzyme reaches a metastable postmethylation state with SAH and m(7)GTP in the active site, and the subsequent covalent transfer of m(7)GMP to nsP1 triggered by the presence of RNA and postdecapping reaction conformational changes inducing the opening of the pore. In addition, we biochemically characterize the capping reaction, demonstrating specificity for the RNA substrate and the reversibility of the cap transfer resulting in decapping activity and the release of reaction intermediates. Our data identify the molecular determinants allowing each pathway transition, providing an explanation for the need for the SAM methyl donor all along the pathway and clues about the conformational rearrangements associated to the enzymatic activity of nsP1. Together, our results set ground for the structural and functional understanding of alphavirus RNA-capping and the design of antivirals.

-	~~Authors: Reguera, J~~., Jones, R., Hons, M.	+	Structural basis and dynamics of Chikungunya alphavirus RNA capping by nsP1 capping pores.,Jones R, Hons M, Rabah N, Zamarreno N, Arranz R, Reguera J Proc Natl Acad Sci U S A. 2023 Mar 21;120(12):e2213934120. doi: , 10.1073/pnas.2213934120. Epub 2023 Mar 13. PMID:36913573<ref>PMID:36913573</ref>

-	~~Description: Structure~~ of ~~an open form~~ of ~~CHIKV nsP1 capping pores~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Reguera, J~~]]	+	<div class="pdbe-citations 8axv" style="background-color:#fffaf0;"></div>
-	[[Category: Hons, M]]	+	== References ==
-	[[Category: Jones, R]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chikungunya virus strain S27-African prototype]]
		+	[[Category: Large Structures]]
		+	[[Category: Hons M]]
		+	[[Category: Jones R]]
		+	[[Category: Reguera J]]

OCA: Protected "8axv" [edit=sysop:move=sysop]

2022-09-07T15:28:33Z

Protected "8axv" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:28, 7 September 2022

OCA: New page: '''Unreleased structure''' The entry 8axv is ON HOLD Authors: Reguera, J., Jones, R., Hons, M. Description: Structure of an open form of CHIKV nsP1 capping pores [[Category: Unreleased...

2022-09-07T15:28:32Z

New page: '''Unreleased structure''' The entry 8axv is ON HOLD Authors: Reguera, J., Jones, R., Hons, M. Description: Structure of an open form of CHIKV nsP1 capping pores [[Category: Unreleased...

New page

'''Unreleased structure'''

The entry 8axv is ON HOLD

Authors: Reguera, J., Jones, R., Hons, M.

Description: Structure of an open form of CHIKV nsP1 capping pores
[[Category: Unreleased Structures]]
[[Category: Reguera, J]]
[[Category: Hons, M]]
[[Category: Jones, R]]

8axv - Revision history

OCA at 06:47, 29 March 2023

OCA: Protected "8axv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8axv is ON HOLD Authors: Reguera, J., Jones, R., Hons, M. Description: Structure of an open form of CHIKV nsP1 capping pores [[Category: Unreleased...