OCA at 08:20, 16 August 2023

2023-08-16T08:20:12Z

←Older revision		Revision as of 08:20, 16 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8bhl is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and Am6AA mRNA codon in the A-site after uncompleted di-peptide formation==
		+	<StructureSection load='8bhl' size='340' side='right'caption='[[8bhl]], [[Resolution\|resolution]] 2.21Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8bhl]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BHL FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.21Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MZ:N6-METHYLADENOSINE-5-MONOPHOSPHATE'>6MZ</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bhl OCA], [https://pdbe.org/8bhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bhl RCSB], [https://www.ebi.ac.uk/pdbsum/8bhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bhl ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RL2_ECOLI RL2_ECOLI] One of the primary rRNA binding proteins. Located near the base of the L1 stalk, it is probably also mobile. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is highly controversial.[HAMAP-Rule:MF_01320_B] In the E.coli 70S ribosome in the initiation state it has been modeled to make several contacts with the 16S rRNA (forming bridge B7b, PubMed:12809609); these contacts are broken in the model with bound EF-G.[HAMAP-Rule:MF_01320_B]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	N(6)-methyladenosine (m(6)A) is an abundant, dynamic mRNA modification that regulates key steps of cellular mRNA metabolism. m(6)A in the mRNA coding regions inhibits translation elongation. Here, we show how m(6)A modulates decoding in the bacterial translation system using a combination of rapid kinetics, smFRET and single-particle cryo-EM. We show that, while the modification does not impair the initial binding of aminoacyl-tRNA to the ribosome, in the presence of m(6)A fewer ribosomes complete the decoding process due to the lower stability of the complexes and enhanced tRNA drop-off. The mRNA codon adopts a pi-stacked codon conformation that is remodeled upon aminoacyl-tRNA binding. m(6)A does not exclude canonical codon-anticodon geometry, but favors alternative more dynamic conformations that are rejected by the ribosome. These results highlight how modifications outside the Watson-Crick edge can still interfere with codon-anticodon base pairing and complex recognition by the ribosome, thereby modulating the translational efficiency of modified mRNAs.

-	~~Authors~~:	+	Modulation of translational decoding by m(6)A modification of mRNA.,Jain S, Koziej L, Poulis P, Kaczmarczyk I, Gaik M, Rawski M, Ranjan N, Glatt S, Rodnina MV Nat Commun. 2023 Aug 8;14(1):4784. doi: 10.1038/s41467-023-40422-7. PMID:37553384<ref>PMID:37553384</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8bhl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Large Structures]]
		+	[[Category: Glatt S]]
		+	[[Category: Koziej L]]

OCA at 04:43, 7 April 2023

2023-04-07T04:43:41Z

←Older revision		Revision as of 04:43, 7 April 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bhl is ON HOLD	+	The entry 8bhl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8bhl" [edit=sysop:move=sysop]

2022-11-09T04:54:25Z

Protected "8bhl" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:54, 9 November 2022

OCA: New page: '''Unreleased structure''' The entry 8bhl is ON HOLD Authors: Description: Category: Unreleased Structures

2022-11-09T04:54:24Z

New page: '''Unreleased structure''' The entry 8bhl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8bhl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8bhl - Revision history

OCA at 08:20, 16 August 2023

OCA at 04:43, 7 April 2023

OCA: Protected "8bhl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8bhl is ON HOLD Authors: Description: Category: Unreleased Structures