OCA at 20:30, 12 April 2023

2023-04-12T20:30:42Z

←Older revision		Revision as of 20:30, 12 April 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8bx7 is ~~ON HOLD until Paper~~ Publication	+	==Structure of the rod CNG channel bound to calmodulin==
		+	<StructureSection load='8bx7' size='340' side='right'caption='[[8bx7]], [[Resolution\|resolution]] 2.76Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8bx7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BX7 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bx7 OCA], [https://pdbe.org/8bx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bx7 RCSB], [https://www.ebi.ac.uk/pdbsum/8bx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bx7 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CNGB1_BOVIN CNGB1_BOVIN] Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calmodulin (CaM) regulates many ion channels to control calcium entry into cells, and mutations that alter this interaction are linked to fatal diseases. The structural basis of CaM regulation remains largely unexplored. In retinal photoreceptors, CaM binds to the CNGB subunit of cyclic nucleotide-gated (CNG) channels and, thereby, adjusts the channel's Cyclic guanosine monophosphate (cGMP) sensitivity in response to changes in ambient light conditions. Here, we provide the structural characterization for CaM regulation of a CNG channel by using a combination of single-particle cryo-electron microscopy and structural proteomics. CaM connects the CNGA and CNGB subunits, resulting in structural changes both in the cytosolic and transmembrane regions of the channel. Cross-linking and limited proteolysis-coupled mass spectrometry mapped the conformational changes induced by CaM in vitro and in the native membrane. We propose that CaM is a constitutive subunit of the rod channel to ensure high sensitivity in dim light. Our mass spectrometry-based approach is generally relevant for studying the effect of CaM on ion channels in tissues of medical interest, where only minute quantities are available.

-	~~Authors~~:	+	Structural basis of calmodulin modulation of the rod cyclic nucleotide-gated channel.,Barret DCA, Schuster D, Rodrigues MJ, Leitner A, Picotti P, Schertler GFX, Kaupp UB, Korkhov VM, Marino J Proc Natl Acad Sci U S A. 2023 Apr 11;120(15):e2300309120. doi: , 10.1073/pnas.2300309120. Epub 2023 Apr 3. PMID:37011209<ref>PMID:37011209</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8bx7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
		+	[[Category: Marino J]]

OCA at 04:15, 2 March 2023

2023-03-02T04:15:21Z

←Older revision		Revision as of 04:15, 2 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bx7 is ON HOLD	+	The entry 8bx7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 05:30, 28 December 2022

2022-12-28T05:30:11Z

←Older revision		Revision as of 05:30, 28 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bx7 is ON HOLD ~~until sometime in the future~~	+	The entry 8bx7 is ON HOLD

	Authors:		Authors:

OCA at 06:05, 21 December 2022

2022-12-21T06:05:15Z

←Older revision		Revision as of 06:05, 21 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bx7 is ON HOLD	+	The entry 8bx7 is ON HOLD until sometime in the future

	Authors:		Authors:

OCA: Protected "8bx7" [edit=sysop:move=sysop]

2022-12-14T07:30:04Z

Protected "8bx7" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:30, 14 December 2022

OCA: New page: '''Unreleased structure''' The entry 8bx7 is ON HOLD Authors: Description: Category: Unreleased Structures

2022-12-14T07:30:03Z

New page: '''Unreleased structure''' The entry 8bx7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page