8c4e - Revision history

OCA at 10:03, 17 January 2024

2024-01-17T10:03:06Z

←Older revision		Revision as of 10:03, 17 January 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8c4e is ~~ON HOLD until 2025~~-01-03	+	==F-actin decorated by SipA426-685==
		+	<StructureSection load='8c4e' size='340' side='right'caption='[[8c4e]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c4e]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Salmonella Salmonella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C4E FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c4e OCA], [https://pdbe.org/8c4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c4e RCSB], [https://www.ebi.ac.uk/pdbsum/8c4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c4e ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ACTS_CHICK ACTS_CHICK] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Secreted bacterial type III secretion system (T3SS) proteins are essential for successful infection by many human pathogens. Both T3SS translocator SipC and effector SipA are critical for Salmonella infection by subversion of the host cell cytoskeleton, but the precise molecular interplay between them remains unknown. Here, using cryo-electron microscopy, we show that SipA binds along the F-actin grooves with a unique binding pattern. SipA stabilizes F-actin through charged interface residues and appears to prevent inorganic phosphate release through closure of the "back door" of adenosine 5'-triphosphate pocket. We also show that SipC enhances the binding of SipA to F-actin, thus demonstrating that a sequential presence of T3SS proteins in host cells is associated with a sequence of infection events-starting with actin nucleation, filament growth, and stabilization. Together, our data explain the coordinated interplay of a precisely tuned and highly effective mechanism during Salmonella infection and provide a blueprint for interfering with Salmonella effectors acting on actin.

-	~~Authors: Yuan~~, B., Wald, J., Marlovits~~, T~~.C.	+	Structural basis for subversion of host cell actin cytoskeleton during Salmonella infection.,Yuan B, Scholz J, Wald J, Thuenauer R, Hennell James R, Ellenberg I, Windhorst S, Faix J, Marlovits TC Sci Adv. 2023 Dec 8;9(49):eadj5777. doi: 10.1126/sciadv.adj5777. Epub 2023 Dec 8. PMID:38064550<ref>PMID:38064550</ref>

-	~~Description: F~~-~~actin decorated by SipA426~~-~~685~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Yuan, B~~]]	+	<div class="pdbe-citations 8c4e" style="background-color:#fffaf0;"></div>
-	[[Category: Marlovits~~, T.C~~]]	+	== References ==
-	[[Category: Wald, J]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
		+	[[Category: Salmonella]]
		+	[[Category: Marlovits TC]]
		+	[[Category: Wald J]]
		+	[[Category: Yuan B]]

OCA at 06:09, 29 November 2023

2023-11-29T06:09:25Z

←Older revision		Revision as of 06:09, 29 November 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c4e is ON HOLD	+	The entry 8c4e is ON HOLD until 2025-01-03

	Authors: Yuan, B., Wald, J., Marlovits, T.C.		Authors: Yuan, B., Wald, J., Marlovits, T.C.

OCA at 05:27, 22 November 2023

2023-11-22T05:27:34Z

←Older revision		Revision as of 05:27, 22 November 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c4e is ON HOLD ~~until 2025-01-03~~	+	The entry 8c4e is ON HOLD

	Authors: Yuan, B., Wald, J., Marlovits, T.C.		Authors: Yuan, B., Wald, J., Marlovits, T.C.

OCA at 05:04, 15 November 2023

2023-11-15T05:04:10Z

←Older revision		Revision as of 05:04, 15 November 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c4e is ON HOLD	+	The entry 8c4e is ON HOLD until 2025-01-03

	Authors: Yuan, B., Wald, J., Marlovits, T.C.		Authors: Yuan, B., Wald, J., Marlovits, T.C.

OCA at 11:46, 20 September 2023

2023-09-20T11:46:52Z

←Older revision		Revision as of 11:46, 20 September 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c4e is ON HOLD ~~until 2025-01-03~~	+	The entry 8c4e is ON HOLD

	Authors: Yuan, B., Wald, J., Marlovits, T.C.		Authors: Yuan, B., Wald, J., Marlovits, T.C.

-	Description: F-actin ~~polymerized~~ by ~~SipA~~-~~C under low-salt conditions~~	+	Description: F-actin decorated by SipA426-685
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Yuan, B]]		[[Category: Yuan, B]]
	[[Category: Marlovits, T.C]]		[[Category: Marlovits, T.C]]
	[[Category: Wald, J]]		[[Category: Wald, J]]

OCA at 04:56, 8 February 2023

2023-02-08T04:56:15Z

←Older revision		Revision as of 04:56, 8 February 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c4e is ON HOLD	+	The entry 8c4e is ON HOLD until 2025-01-03

	Authors: Yuan, B., Wald, J., Marlovits, T.C.		Authors: Yuan, B., Wald, J., Marlovits, T.C.

OCA: Protected "8c4e" [edit=sysop:move=sysop]

2023-01-11T05:43:54Z

Protected "8c4e" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 11 January 2023

OCA: New page: '''Unreleased structure''' The entry 8c4e is ON HOLD Authors: Yuan, B., Wald, J., Marlovits, T.C. Description: F-actin polymerized by SipA-C under low-salt conditions [[Category: Unrel...

2023-01-11T05:43:53Z

New page: '''Unreleased structure''' The entry 8c4e is ON HOLD Authors: Yuan, B., Wald, J., Marlovits, T.C. Description: F-actin polymerized by SipA-C under low-salt conditions [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 8c4e is ON HOLD

Authors: Yuan, B., Wald, J., Marlovits, T.C.

Description: F-actin polymerized by SipA-C under low-salt conditions
[[Category: Unreleased Structures]]
[[Category: Yuan, B]]
[[Category: Marlovits, T.C]]
[[Category: Wald, J]]