OCA at 04:02, 25 May 2023

2023-05-25T04:02:15Z

←Older revision		Revision as of 04:02, 25 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8c5d is ~~ON HOLD until Paper~~ Publication	+	==Glutathione transferase P1-1 from Mus musculus==
		+	<StructureSection load='8c5d' size='340' side='right'caption='[[8c5d]], [[Resolution\|resolution]] 1.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C5D FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTB:S-(P-NITROBENZYL)GLUTATHIONE'>GTB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c5d OCA], [https://pdbe.org/8c5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c5d RCSB], [https://www.ebi.ac.uk/pdbsum/8c5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c5d ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GSTP1_MOUSE GSTP1_MOUSE] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Can metabolize 1-chloro-2,4-dinitrobenzene. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multidrug resistance is a significant barrier that makes anticancer therapies less effective. Glutathione transferases (GSTs) are involved in multidrug resistance mechanisms and play a significant part in the metabolism of alkylating anticancer drugs. The purpose of this study was to screen and select a lead compound with high inhibitory potency against the isoenzyme GSTP1-1 from Mus musculus (MmGSTP1-1). The lead compound was selected following the screening of a library of currently approved and registered pesticides that belong to different chemical classes. The results showed that the fungicide iprodione [3-(3,5-dichlorophenyl)-2,4-dioxo-N-propan-2-ylimidazolidine-1-carboxamide] exhibited the highest inhibition potency (IotaC(50) = 11.3 +/- 0.5 muMu) towards MmGSTP1-1. Kinetics analysis revealed that iprodione functions as a mixed-type inhibitor towards glutathione (GSH) and non-competitive inhibitor towards 1-chloro-2,4-dinitrobenzene (CDNB). X-ray crystallography was used to determine the crystal structure of MmGSTP1-1 at 1.28 A resolution as a complex with S-(p-nitrobenzyl)glutathione (Nb-GSH). The crystal structure was used to map the ligand-binding site of MmGSTP1-1 and to provide structural data of the interaction of the enzyme with iprodione using molecular docking. The results of this study shed light on the inhibition mechanism of MmGSTP1-1 and provide a new compound as a potential lead structure for future drug/inhibitor development.

-	~~Authors:~~ Papageorgiou, A.C.	+	Inhibition Analysis and High-Resolution Crystal Structure of Mus musculus Glutathione Transferase P1-1.,Kupreienko O, Pouliou F, Konstandinidis K, Axarli I, Douni E, Papageorgiou AC, Labrou NE Biomolecules. 2023 Mar 29;13(4):613. doi: 10.3390/biom13040613. PMID:37189361<ref>PMID:37189361</ref>

-	~~Description: Glutathione transferase P1~~-~~1 from Mus musculus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Papageorgiou~~, A.C~~]]	+	<div class="pdbe-citations 8c5d" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Papageorgiou AC]]

OCA at 09:08, 1 February 2023

2023-02-01T09:08:12Z

←Older revision		Revision as of 09:08, 1 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c5d is ON HOLD	+	The entry 8c5d is ON HOLD until Paper Publication

	Authors: Papageorgiou, A.C.		Authors: Papageorgiou, A.C.

OCA: Protected "8c5d" [edit=sysop:move=sysop]

2023-01-18T04:55:01Z

Protected "8c5d" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 18 January 2023

OCA: New page: '''Unreleased structure''' The entry 8c5d is ON HOLD Authors: Papageorgiou, A.C. Description: Glutathione transferase P1-1 from Mus musculus Category: Unreleased Structures [[Categ...

2023-01-18T04:54:59Z

New page: '''Unreleased structure''' The entry 8c5d is ON HOLD Authors: Papageorgiou, A.C. Description: Glutathione transferase P1-1 from Mus musculus Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 8c5d is ON HOLD

Authors: Papageorgiou, A.C.

Description: Glutathione transferase P1-1 from Mus musculus
[[Category: Unreleased Structures]]
[[Category: Papageorgiou, A.C]]

8c5d - Revision history

OCA at 04:02, 25 May 2023

OCA at 09:08, 1 February 2023

OCA: Protected "8c5d" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8c5d is ON HOLD Authors: Papageorgiou, A.C. Description: Glutathione transferase P1-1 from Mus musculus Category: Unreleased Structures [[Categ...