OCA at 06:27, 7 April 2023

2023-04-07T06:27:28Z

←Older revision		Revision as of 06:27, 7 April 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8c8x is ~~ON HOLD~~	+	==Cryo-EM captures early ribosome assembly in action==
		+	<StructureSection load='8c8x' size='340' side='right'caption='[[8c8x]], [[Resolution\|resolution]] 3.93Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c8x]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C8X FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c8x OCA], [https://pdbe.org/8c8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c8x RCSB], [https://www.ebi.ac.uk/pdbsum/8c8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c8x ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RL2_ECOLI RL2_ECOLI] One of the primary rRNA binding proteins. Located near the base of the L1 stalk, it is probably also mobile. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is highly controversial.[HAMAP-Rule:MF_01320_B] In the E.coli 70S ribosome in the initiation state it has been modeled to make several contacts with the 16S rRNA (forming bridge B7b, PubMed:12809609); these contacts are broken in the model with bound EF-G.[HAMAP-Rule:MF_01320_B]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal proteins. To obtain insights into the still unexplored early assembly phase of the bacterial 50S subunit, we exploited a minimal in vitro reconstitution system using purified ribosomal components and scalable reaction conditions. Time-limited assembly assays combined with cryo-EM analysis visualizes the structurally complex assembly pathway starting with a particle consisting of ordered density for only ~500 nucleotides of 23S rRNA domain I and three ribosomal proteins. In addition, our structural analysis reveals that early 50S assembly occurs in a domain-wise fashion, while late 50S assembly proceeds incrementally. Furthermore, we find that both ribosomal proteins and folded rRNA helices, occupying surface exposed regions on pre-50S particles, induce, or stabilize rRNA folds within adjacent regions, thereby creating cooperativity.

-	~~Authors~~:	+	Cryo-EM captures early ribosome assembly in action.,Qin B, Lauer SM, Balke A, Vieira-Vieira CH, Burger J, Mielke T, Selbach M, Scheerer P, Spahn CMT, Nikolay R Nat Commun. 2023 Feb 17;14(1):898. doi: 10.1038/s41467-023-36607-9. PMID:36797249<ref>PMID:36797249</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8c8x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Lauer S]]
		+	[[Category: Nikolay R]]
		+	[[Category: Qin B]]

OCA: Protected "8c8x" [edit=sysop:move=sysop]

2023-02-01T09:10:44Z

Protected "8c8x" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:10, 1 February 2023

OCA: New page: '''Unreleased structure''' The entry 8c8x is ON HOLD Authors: Description: Category: Unreleased Structures

2023-02-01T09:10:43Z

New page: '''Unreleased structure''' The entry 8c8x is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8c8x is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8c8x - Revision history

OCA at 06:27, 7 April 2023

OCA: Protected "8c8x" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8c8x is ON HOLD Authors: Description: Category: Unreleased Structures