OCA at 11:39, 13 December 2023

2023-12-13T11:39:17Z

←Older revision		Revision as of 11:39, 13 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8clf is ~~ON HOLD~~	+	==Z-SolQ2Br bound to tubulin (T2R-TTL) complex==
		+	<StructureSection load='8clf' size='340' side='right'caption='[[8clf]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8clf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CLF FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=V1O:5-[[4-(2-bromoethyl)-3,5-dimethoxy-phenyl]diazenyl]-2-methoxy-phenol'>V1O</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8clf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8clf OCA], [https://pdbe.org/8clf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8clf RCSB], [https://www.ebi.ac.uk/pdbsum/8clf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8clf ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such experiments. Here, we describe a multi-reservoir, high viscosity extruder as a step towards automation of sample delivery at XFELs. Compared to a standard single extruder, sample exchange time was halved and the workload of users was greatly reduced. In-built temperature control of samples facilitated optimal extrusion and supported sample stability. After commissioning the device with lysozyme crystals, we collected time-resolved data using crystals of a membrane-bound, light-driven sodium pump. Static data were also collected from the soluble protein tubulin that was soaked with a series of small molecule drugs. Using these data, we identify low occupancy (as little as 30%) ligands using a minimal amount of data from a serial crystallography experiment, a result that could be exploited for structure-based drug design.

-	~~Authors~~:	+	A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers.,Wranik M, Kepa MW, Beale EV, James D, Bertrand Q, Weinert T, Furrer A, Glover H, Gashi D, Carrillo M, Kondo Y, Stipp RT, Khusainov G, Nass K, Ozerov D, Cirelli C, Johnson PJM, Dworkowski F, Beale JH, Stubbs S, Zamofing T, Schneider M, Krauskopf K, Gao L, Thorn-Seshold O, Bostedt C, Bacellar C, Steinmetz MO, Milne C, Standfuss J Nat Commun. 2023 Dec 2;14(1):7956. doi: 10.1038/s41467-023-43523-5. PMID:38042952<ref>PMID:38042952</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8clf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Bertrand Q]]
		+	[[Category: Kepa M]]
		+	[[Category: Standfuss J]]
		+	[[Category: Steinmetz M]]
		+	[[Category: Weinert T]]
		+	[[Category: Wranik M]]

OCA: Protected "8clf" [edit=sysop:move=sysop]

2023-02-22T09:54:30Z

Protected "8clf" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:54, 22 February 2023

OCA: New page: '''Unreleased structure''' The entry 8clf is ON HOLD Authors: Description: Category: Unreleased Structures

2023-02-22T09:54:27Z

New page: '''Unreleased structure''' The entry 8clf is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8clf is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8clf - Revision history

OCA at 11:39, 13 December 2023

OCA: Protected "8clf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8clf is ON HOLD Authors: Description: Category: Unreleased Structures