8co4 - Revision history

OCA at 19:48, 29 May 2024

2024-05-29T19:48:15Z

←Older revision		Revision as of 19:48, 29 May 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ADHX_ARATH ADHX_ARATH] Plays a central role in formaldehyde detoxification.<ref>PMID:12913179</ref>		[https://www.uniprot.org/uniprot/ADHX_ARATH ADHX_ARATH] Plays a central role in formaldehyde detoxification.<ref>PMID:12913179</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Alcohol dehydrogenases (ADHs) are a group of zinc-binding enzymes belonging to the medium-length dehydrogenase/reductase (MDR) protein superfamily. In plants, these enzymes fulfill important functions involving the reduction of toxic aldehydes to the corresponding alcohols (as well as catalyzing the reverse reaction, i.e., alcohol oxidation; ADH1) and the reduction of nitrosoglutathione (GSNO; ADH2/GSNOR). We investigated and compared the structural and biochemical properties of ADH1 and GSNOR from Arabidopsis thaliana. We expressed and purified ADH1 and GSNOR and determined two new structures, NADH-ADH1 and apo-GSNOR, thus completing the structural landscape of Arabidopsis ADHs in both apo- and holo-forms. A structural comparison of these Arabidopsis ADHs revealed a high sequence conservation (59% identity) and a similar fold. In contrast, a striking dissimilarity was observed in the catalytic cavity supporting substrate specificity and accommodation. Consistently, ADH1 and GSNOR showed strict specificity for their substrates (ethanol and GSNO, respectively), although both enzymes had the ability to oxidize long-chain alcohols, with ADH1 performing better than GSNOR. Both enzymes contain a high number of cysteines (12 and 15 out of 379 residues for ADH1 and GSNOR, respectively) and showed a significant and similar responsivity to thiol-oxidizing agents, indicating that redox modifications may constitute a mechanism for controlling enzyme activity under both optimal growth and stress conditions.
		+
		+	Structural and biochemical characterization of Arabidopsis alcohol dehydrogenases reveals distinct functional properties but similar redox sensitivity.,Meloni M, Rossi J, Fanti S, Carloni G, Tedesco D, Treffon P, Piccinini L, Falini G, Trost P, Vierling E, Licausi F, Giuntoli B, Musiani F, Fermani S, Zaffagnini M Plant J. 2024 May;118(4):1054-1070. doi: 10.1111/tpj.16651. Epub 2024 Feb 2. PMID:38308388<ref>PMID:38308388</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8co4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:54, 21 February 2024

2024-02-21T08:54:23Z

←Older revision		Revision as of 08:54, 21 February 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8co4 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of apo S-nitrosoglutathione reductase from Arabidopsis thalina==
-		+	<StructureSection load='8co4' size='340' side='right'caption='[[8co4]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors~~: ~~Fermani~~, S., ~~Fanti~~, S., ~~Carloni~~, G., ~~Rossi~~, J., ~~Falini~~, G., ~~Zaffagnini, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8co4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CO4 FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of apo S-nitrosoglutathione reductase from Arabidopsis thalina~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Fermani, S~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8co4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8co4 OCA], [https://pdbe.org/8co4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8co4 RCSB], [https://www.ebi.ac.uk/pdbsum/8co4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8co4 ProSAT]</span></td></tr>
-	[[Category: ~~Zaffagnini, M~~]]	+	</table>
-	[[Category: Falini, G]]	+	== Function ==
-	[[Category: Fanti, S]]	+	[https://www.uniprot.org/uniprot/ADHX_ARATH ADHX_ARATH] Plays a central role in formaldehyde detoxification.<ref>PMID:12913179</ref>
-	[[Category: Rossi, J]]	+	== References ==
-	[[Category: ~~Carloni, G~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Large Structures]]
		+	[[Category: Carloni G]]
		+	[[Category: Falini G]]
		+	[[Category: Fanti S]]
		+	[[Category: Fermani S]]
		+	[[Category: Rossi J]]
		+	[[Category: Zaffagnini M]]

OCA at 07:30, 15 March 2023

2023-03-15T07:30:42Z

←Older revision		Revision as of 07:30, 15 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8co4 is ON HOLD	+	The entry 8co4 is ON HOLD until Paper Publication

	Authors: Fermani, S., Fanti, S., Carloni, G., Rossi, J., Falini, G., Zaffagnini, M.		Authors: Fermani, S., Fanti, S., Carloni, G., Rossi, J., Falini, G., Zaffagnini, M.
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	[[Category: Zaffagnini, M]]		[[Category: Zaffagnini, M]]
	[[Category: Falini, G]]		[[Category: Falini, G]]
-	[[Category: Rossi, J]]
	[[Category: Fanti, S]]		[[Category: Fanti, S]]
		+	[[Category: Rossi, J]]
	[[Category: Carloni, G]]		[[Category: Carloni, G]]

OCA: Protected "8co4" [edit=sysop:move=sysop]

2023-03-08T05:36:15Z

Protected "8co4" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:36, 8 March 2023

OCA: New page: '''Unreleased structure''' The entry 8co4 is ON HOLD Authors: Fermani, S., Fanti, S., Carloni, G., Rossi, J., Falini, G., Zaffagnini, M. Description: Crystal structure of apo S-nitroso...

2023-03-08T05:36:14Z

New page: '''Unreleased structure''' The entry 8co4 is ON HOLD Authors: Fermani, S., Fanti, S., Carloni, G., Rossi, J., Falini, G., Zaffagnini, M. Description: Crystal structure of apo S-nitroso...

New page

'''Unreleased structure'''

The entry 8co4 is ON HOLD

Authors: Fermani, S., Fanti, S., Carloni, G., Rossi, J., Falini, G., Zaffagnini, M.

Description: Crystal structure of apo S-nitrosoglutathione reductase from Arabidopsis thalina
[[Category: Unreleased Structures]]
[[Category: Fermani, S]]
[[Category: Zaffagnini, M]]
[[Category: Falini, G]]
[[Category: Rossi, J]]
[[Category: Fanti, S]]
[[Category: Carloni, G]]