8cpa - Revision history

OCA at 06:14, 5 August 2016

2016-08-05T06:14:16Z

←Older revision		Revision as of 06:14, 5 August 2016
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		+
	==COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY==		==COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY==
	<StructureSection load='8cpa' size='340' side='right' caption='[[8cpa]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='8cpa' size='340' side='right' caption='[[8cpa]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[8cpa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8CPA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[8cpa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8CPA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AGF:O-(((1R)-((N-(PHENYL-METHOXY-CARBONYL)-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE'>AGF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AGF:O-(((1R)-((N-(PHENYL-METHOXY-CARBONYL)-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE'>AGF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cpa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8cpa RCSB], [http://www.ebi.ac.uk/pdbsum/8cpa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cpa OCA], [http://pdbe.org/8cpa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=8cpa RCSB], [http://www.ebi.ac.uk/pdbsum/8cpa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=8cpa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=8cpa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 8cpa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
	[[Category: Carboxypeptidase A]]		[[Category: Carboxypeptidase A]]
	[[Category: Kim, H]]		[[Category: Kim, H]]
	[[Category: Lipscomb, W N]]		[[Category: Lipscomb, W N]]

OCA at 16:26, 25 December 2014

2014-12-25T16:26:09Z

←Older revision		Revision as of 16:26, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cpa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8cpa RCSB], [http://www.ebi.ac.uk/pdbsum/8cpa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cpa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8cpa RCSB], [http://www.ebi.ac.uk/pdbsum/8cpa PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CBPA1_BOVIN CBPA1_BOVIN]] Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:57, 10 December 2014

2014-12-10T16:57:02Z

←Older revision		Revision as of 16:57, 10 December 2014
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-	[[~~Image~~:8cpa.~~png\|left~~\|200px]]	+	==COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY==
		+	<StructureSection load='8cpa' size='340' side='right' caption='[[8cpa]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8cpa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8CPA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AGF:O-(((1R)-((N-(PHENYL-METHOXY-CARBONYL)-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE'>AGF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cpa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8cpa RCSB], [http://www.ebi.ac.uk/pdbsum/8cpa PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/8cpa_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structures of the complexes of carboxypeptidase A (CPA) with two tight-binding phosphonate inhibitors have been determined by X-ray crystallography. The inhibitors, Cbz-Phe-ValP-(O)-Phe[ZFVP(O)F] and Cbz-Ala-GlyP-(O)-Phe[ZAGP(O)F], bind noncovalently to CPA with dissociation constants (Ki's) of 11 fM and 710 pM, respectively. The CPA-ZFVP(O)F complex crystallizes in the space group P2(1)2(1)2(1) with unit cell parameters a = 65.3 A, b = 63.4 A, and c = 76.0 A, and the CPA-ZAGP(O)F complex crystallizes in the space group P2(1)2(1)2(1) with unit cell parameters a = 63.4 A, b = 65.9 A, and c = 74.4 A. Both structures were determined by molecular replacement to a resolution of 2.0 A. The final crystallographic residuals are 0.189 for the CPA-ZFVP(O)F complex and 0.191 for the CPA-ZAGP(O)F complex. The CPA-ZFVP(O)F complex exhibits the lowest Ki yet determined for an enzyme-inhibitor interaction. Comparison of the CPA-ZFVP(O)F structure with that of the CPA-ZAAP(O)F complex [Kim, H., & Lipscomb, W.N. (1990) Biochemistry 29, 5546-5555] indicates the likely important contributions of hydrophobic and weakly polar enzyme-inhibitor interactions to the exceptional stability of the CPA-ZFVP(O)F complex. Among these interactions is a network of four aromatic rings of CPA and ZFVP(O)F in a configuration that allows stabilizing aromatic-aromatic edge-to-face interactions from one ring to the next. A comparison of the structures of the CPA-ZFVP(O)F, CPA-ZAAP(O)F and CPA-ZAGP(O)F complexes shows that all three phosphonates assume a similar binding mode in the active-site binding groove of CPA. For ZAGP(O)F, the glycyl P1 residue does not lead to an anomalous or a partially disordered binding mode as seen in some previous complexes of CPA involving dipeptide analogue inhibitors with glycyl P1 residues. The additional enzyme-inhibitor interactions for these tripeptide phosphonates secure a binding mode in which a Pi portion of the inhibitor is clearly bound by the corresponding Si binding subsite. These three phosphonates have been implicated as transition-state analogues of the CPA-catalyzed reaction. The phosphinyl groups of these phosphonates coordinate to the active-site zinc in a manner that has been proposed as a characteristic feature of the general-base (Zn-hydroxyl or Zn-water) mechanism for the CPA-catalyzed reaction. Further mechanistic proposals are made for Arg-127, whose probable role in binding substrates is apparent in these CPA-phosphonate complexes.

-	~~{{STRUCTURE_8cpa\| PDB=8cpa \| SCENE= }}~~	+	Comparison of the structures of three carboxypeptidase A-phosphonate complexes determined by X-ray crystallography.,Kim H, Lipscomb WN Biochemistry. 1991 Aug 20;30(33):8171-80. PMID:1868092<ref>PMID:1868092</ref>

-	~~===COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_1868092}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[8cpa]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA].~~	+

	==See Also==		==See Also==
	*[[Carboxypeptidase\|Carboxypeptidase]]		*[[Carboxypeptidase\|Carboxypeptidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:001868092<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Carboxypeptidase A]]		[[Category: Carboxypeptidase A]]
-	[[Category: Kim, H.]]	+	[[Category: Kim, H]]
-	[[Category: Lipscomb, W N.]]	+	[[Category: Lipscomb, W N]]

OCA at 18:53, 20 October 2012

2012-10-20T18:53:00Z

←Older revision		Revision as of 18:53, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[8cpa]] is a 1 chain structure ~~of [[Carboxypeptidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA].	+	[[8cpa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA].

	==See Also==		==See Also==

OCA: Protected "8cpa" [edit=sysop:move=sysop]

2012-07-27T05:34:23Z

Protected "8cpa" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:34, 27 July 2012

OCA at 05:34, 27 July 2012

2012-07-27T05:34:21Z

←Older revision		Revision as of 05:34, 27 July 2012
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-	{{Seed}}
	[[Image:8cpa.png\|left\|200px]]		[[Image:8cpa.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_8cpa", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_8cpa\| PDB=8cpa \| SCENE= }}		{{STRUCTURE_8cpa\| PDB=8cpa \| SCENE= }}

	===COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY===		===COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_1868092}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 1868092 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_1868092}}		{{ABSTRACT_PUBMED_1868092}}

	==About this Structure==		==About this Structure==
-	~~8CPA~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA].	+	[[8cpa]] is a 1 chain structure of [[Carboxypeptidase]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA].
		+
		+	==See Also==
		+	*[[Carboxypeptidase\|Carboxypeptidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~1868092~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:001868092</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Carboxypeptidase A]]		[[Category: Carboxypeptidase A]]
	[[Category: Kim, H.]]		[[Category: Kim, H.]]
	[[Category: Lipscomb, W N.]]		[[Category: Lipscomb, W N.]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:42:44 2009''

OCA at 19:42, 17 February 2009

2009-02-17T19:42:45Z

←Older revision		Revision as of 19:42, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	8CPA is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA].	+	8CPA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CPA OCA].

	==Reference==		==Reference==
-	~~Comparison of the structures of three carboxypeptidase A-phosphonate complexes determined by X-ray crystallography., Kim H, Lipscomb WN, Biochemistry. 1991 Aug 20;30(33):8171-80.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/1868092 1868092]~~	+	<ref group="xtra">PMID:1868092</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Carboxypeptidase A]]		[[Category: Carboxypeptidase A]]
-	[[Category: Single protein]]
	[[Category: Kim, H.]]		[[Category: Kim, H.]]
	[[Category: Lipscomb, W N.]]		[[Category: Lipscomb, W N.]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri Jul 11 12~~:49:~~48 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:42:44 2009''

OCA at 09:49, 11 July 2008

2008-07-11T09:49:49Z

←Older revision		Revision as of 09:49, 11 July 2008
Line 1:		Line 1:
-	[[Image:8cpa.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:8cpa.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_8cpa\| PDB=8cpa \| SCENE= }}		{{STRUCTURE_8cpa\| PDB=8cpa \| SCENE= }}

-	~~'''~~COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY~~'''~~	+	===COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY===


-	~~==Overview==~~	+	<!--
-	~~The structures of the complexes of carboxypeptidase A (CPA) with two tight~~-~~binding phosphonate inhibitors have been determined by X~~-~~ray crystallography.~~ The ~~inhibitors~~, ~~Cbz-Phe-ValP-(O)-Phe[ZFVP(O)F] and Cbz-Ala-GlyP-(O)-Phe[ZAGP(O)F]~~, ~~bind noncovalently to CPA with dissociation constants (Ki's) of 11 fM and 710 pM, respectively. The CPA-ZFVP(O)F complex crystallizes in~~ the space group P2(1)2(1)2(1) with unit cell parameters a = 65.3 A, b = 63.4 A, and c = 76.0 A, and the CPA-ZAGP(O)F complex crystallizes in the space group P2(1)2(1)2(1) with unit cell parameters a = 63.4 A, b = 65.9 A, and c = 74.4 A. Both structures were determined by molecular replacement to ~~a resolution of 2.0 A. The final crystallographic residuals are 0.189 for~~ the ~~CPA-ZFVP~~(~~O)F complex and 0~~.~~191 for the CPA-ZAGP(O)F complex~~. ~~The CPA-ZFVP(O~~)~~F complex exhibits the lowest Ki yet determined for an enzyme-inhibitor interaction. Comparison of the CPA-ZFVP(O)F structure with that of the CPA-ZAAP(O)F complex [Kim~~, H., & Lipscomb, W.N. (1990) Biochemistry 29, 5546-5555] indicates the likely important contributions of hydrophobic and weakly polar enzyme-inhibitor interactions to the exceptional stability of the CPA-ZFVP(O)F complex. Among these interactions is ~~a network of four aromatic rings of CPA and ZFVP(O)F in a configuration that allows stabilizing aromatic-aromatic edge-to-face interactions from one ring to~~ the ~~next~~. ~~A comparison of the structures of the CPA~~-~~ZFVP(O)F, CPA~~-ZAAP(O)F and CPA-ZAGP(O)F complexes shows that all three phosphonates assume a similar binding mode in the active-site binding groove of CPA. For ZAGP(O)F, the glycyl P1 residue does not lead to an anomalous or a partially disordered binding mode as seen in some previous complexes of CPA involving dipeptide analogue inhibitors with glycyl P1 residues. The additional enzyme-inhibitor interactions for these tripeptide phosphonates secure a binding mode in which a Pi portion of the inhibitor is clearly bound by the corresponding Si binding subsite. These three phosphonates have been implicated as transition-state analogues of the CPA-catalyzed reaction. The phosphinyl groups of these phosphonates coordinate to the active-site zinc in a manner that has been proposed as a characteristic feature of the general-base (Zn-hydroxyl or Zn-water) mechanism for the CPA-catalyzed reaction. Further mechanistic proposals are made for Arg-127, whose probable role in binding substrates is apparent in these CPA-phosphonate complexes.	+	The line below this paragraph, {{ABSTRACT_PUBMED_1868092}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 1868092 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_1868092}}

	==About this Structure==		==About this Structure==
Line 25:		Line 29:
	[[Category: Kim, H.]]		[[Category: Kim, H.]]
	[[Category: Lipscomb, W N.]]		[[Category: Lipscomb, W N.]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 22~~:49:04 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Jul 11 12:49:48 2008''

OCA at 19:49, 4 May 2008

2008-05-04T19:49:05Z

←Older revision		Revision as of 19:49, 4 May 2008
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	[[Image:8cpa.gif\|left\|200px]]		[[Image:8cpa.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 8cpa \|SIZE=350\|CAPTION= <scene name='initialview01'>8cpa</scene>~~, ~~resolution 2~~.~~0Å~~	+	The line below this paragraph, containing "STRUCTURE_8cpa", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=AGF:O-~~(~~((1R~~)-(~~(N-(PHENYL-METHOXY-CARBONYL~~)~~-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE'>AGF</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en~~.~~wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span~~>	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	\|~~DOMAIN~~=	+	{{STRUCTURE_8cpa\| PDB=8cpa \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cpa OCA], [http://www.ebi.ac.uk/pdbsum/8cpa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=8cpa RCSB]</span>	+
-	}}	+

	'''COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY'''		'''COMPARISON OF THE STRUCTURES OF THREE CARBOXYPEPTIDASE A-PHOSPHONATE COMPLEXES DETERMINED BY X-RAY CRYSTALLOGRAPHY'''
Line 28:		Line 25:
	[[Category: Kim, H.]]		[[Category: Kim, H.]]
	[[Category: Lipscomb, W N.]]		[[Category: Lipscomb, W N.]]
-	~~[[Category: hydrolase(c-terminal peptidase)]]~~	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:49:04 2008''
-		+
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:45:06 2008''	+

OCA at 02:45, 31 March 2008

2008-03-31T02:45:07Z

←Older revision		Revision as of 02:45, 31 March 2008
Line 4:		Line 4:
	\|PDB= 8cpa \|SIZE=350\|CAPTION= <scene name='initialview01'>8cpa</scene>, resolution 2.0Å		\|PDB= 8cpa \|SIZE=350\|CAPTION= <scene name='initialview01'>8cpa</scene>, resolution 2.0Å
	\|SITE=		\|SITE=
-	\|LIGAND= ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and~~ <scene name='pdbligand=AGF:O-(((1R)-((N-(PHENYL-METHOXY-CARBONYL)-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE'>AGF</scene>	+	\|LIGAND= <scene name='pdbligand=AGF:O-(((1R)-((N-(PHENYL-METHOXY-CARBONYL)-ALANYL)-AMINO)METHYL)HYDROXYPHOSPHINYL)3-L-PHENYLLACTATE'>AGF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
-	\|ACTIVITY= [http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1]	+	\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span>
	\|GENE=		\|GENE=
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cpa OCA], [http://www.ebi.ac.uk/pdbsum/8cpa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=8cpa RCSB]</span>
	}}		}}

Line 25:		Line 28:
	[[Category: Kim, H.]]		[[Category: Kim, H.]]
	[[Category: Lipscomb, W N.]]		[[Category: Lipscomb, W N.]]
-	[[Category: AGF]]
-	[[Category: ZN]]
	[[Category: hydrolase(c-terminal peptidase)]]		[[Category: hydrolase(c-terminal peptidase)]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19~~:15:22 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:45:06 2008''