OCA at 06:58, 18 May 2023

2023-05-18T06:58:50Z

←Older revision		Revision as of 06:58, 18 May 2023
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-	'''Unreleased structure'''

-	~~The entry 8csj is ON HOLD~~	+	==Cryo-EM structure of NTD-directed non-neutralizing antibody 4-33 in complex with prefusion SARS-CoV-2 spike glycoprotein==
-		+	<StructureSection load='8csj' size='340' side='right'caption='[[8csj]], [[Resolution\|resolution]] 3.53Å' scene=''>
-	~~Authors: Reddem, E.R., Shapiro, L.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8csj]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CSJ FirstGlance]. <br>
-	~~Description:~~ Cryo-EM structure of NTD-directed non-neutralizing antibody 4-33 in complex with prefusion SARS-CoV-2 spike glycoprotein	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8csj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8csj OCA], [https://pdbe.org/8csj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8csj RCSB], [https://www.ebi.ac.uk/pdbsum/8csj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8csj ProSAT]</span></td></tr>
-	[[Category: ~~Shapiro, L~~]]	+	</table>
-	[[Category: Reddem~~, E.R~~]]	+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Reddem ER]]
		+	[[Category: Shapiro L]]

OCA: Protected "8csj" [edit=sysop:move=sysop]

2022-05-18T09:24:08Z

Protected "8csj" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:24, 18 May 2022

OCA: New page: '''Unreleased structure''' The entry 8csj is ON HOLD Authors: Reddem, E.R., Shapiro, L. Description: Cryo-EM structure of NTD-directed non-neutralizing antibody 4-33 in complex with pr...

2022-05-18T09:24:07Z

New page: '''Unreleased structure''' The entry 8csj is ON HOLD Authors: Reddem, E.R., Shapiro, L. Description: Cryo-EM structure of NTD-directed non-neutralizing antibody 4-33 in complex with pr...

New page

'''Unreleased structure'''

The entry 8csj is ON HOLD

Authors: Reddem, E.R., Shapiro, L.

Description: Cryo-EM structure of NTD-directed non-neutralizing antibody 4-33 in complex with prefusion SARS-CoV-2 spike glycoprotein
[[Category: Unreleased Structures]]
[[Category: Shapiro, L]]
[[Category: Reddem, E.R]]

8csj - Revision history

OCA at 06:58, 18 May 2023

OCA: Protected "8csj" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8csj is ON HOLD Authors: Reddem, E.R., Shapiro, L. Description: Cryo-EM structure of NTD-directed non-neutralizing antibody 4-33 in complex with pr...