8d8n - Revision history

OCA at 19:02, 7 September 2022

2022-09-07T19:02:37Z

←Older revision		Revision as of 19:02, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]]		[[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Type III-E RNA-targeting effector complex (gRAMP/Cas7-11) is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here we use cryo-electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5' region of the guide RNA displaces a gating loop in gRAMP, which triggers an extensive conformational relay that allosterically aligns the protease catalytic dyad and opens an amino acid sidechain-binding pocket. We further define Csx30 as the endogenous protein substrate that is site-specifically proteolyzed by RNA-activated Craspase. This protease activity is switched off by target RNA cleavage by gRAMP, and is not activated by RNA targets containing a matching protospacer flanking sequence. We thus conclude that Craspase is a target RNA-activated protease with self-regulatory capacity.
		+
		+	Craspase is a CRISPR RNA-guided, RNA-activated protease.,Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodriguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A Science. 2022 Aug 25:eadd5064. doi: 10.1126/science.add5064. PMID:36007061<ref>PMID:36007061</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8d8n" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:51, 7 September 2022

2022-09-07T16:51:55Z

←Older revision		Revision as of 16:51, 7 September 2022
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-	====	+	==gRAMP non-match PFS target RNA==
-	<StructureSection load='8d8n' size='340' side='right'caption='[[8d8n]]' scene=''>	+	<StructureSection load='8d8n' size='340' side='right'caption='[[8d8n]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[8d8n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D8N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d8n OCA], [https://pdbe.org/8d8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d8n RCSB], [https://www.ebi.ac.uk/pdbsum/8d8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d8n ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d8n OCA], [https://pdbe.org/8d8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d8n RCSB], [https://www.ebi.ac.uk/pdbsum/8d8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d8n ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candidatus Scalindua brodae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Hu C]]
		+	[[Category: Ke A]]
		+	[[Category: Nam KH]]
		+	[[Category: Schuler G]]

OCA at 06:40, 31 August 2022

2022-08-31T06:40:14Z

←Older revision		Revision as of 06:40, 31 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8d8n ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='8d8n' size='340' side='right'caption='[[8d8n]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d8n OCA], [https://pdbe.org/8d8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d8n RCSB], [https://www.ebi.ac.uk/pdbsum/8d8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d8n ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:22, 20 July 2022

2022-07-20T04:22:05Z

←Older revision		Revision as of 04:22, 20 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8d8n is ON HOLD	+	The entry 8d8n is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8d8n" [edit=sysop:move=sysop]

2022-06-15T04:15:33Z

Protected "8d8n" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:15, 15 June 2022

OCA: New page: '''Unreleased structure''' The entry 8d8n is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-15T04:15:32Z

New page: '''Unreleased structure''' The entry 8d8n is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8d8n is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]