OCA at 08:17, 14 June 2023

2023-06-14T08:17:25Z

←Older revision		Revision as of 08:17, 14 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8d9e is ~~ON HOLD~~	+	==gRAMP-match PFS target==
		+	<StructureSection load='8d9e' size='340' side='right'caption='[[8d9e]], [[Resolution\|resolution]] 3.76Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8d9e]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D9E FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d9e OCA], [https://pdbe.org/8d9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d9e RCSB], [https://www.ebi.ac.uk/pdbsum/8d9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d9e ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Type III-E RNA-targeting effector complex (gRAMP/Cas7-11) is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here we use cryo-electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5' region of the guide RNA displaces a gating loop in gRAMP, which triggers an extensive conformational relay that allosterically aligns the protease catalytic dyad and opens an amino acid sidechain-binding pocket. We further define Csx30 as the endogenous protein substrate that is site-specifically proteolyzed by RNA-activated Craspase. This protease activity is switched off by target RNA cleavage by gRAMP, and is not activated by RNA targets containing a matching protospacer flanking sequence. We thus conclude that Craspase is a target RNA-activated protease with self-regulatory capacity.

-	~~Authors~~:	+	Craspase is a CRISPR RNA-guided, RNA-activated protease.,Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodriguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A Science. 2022 Aug 25:eadd5064. doi: 10.1126/science.add5064. PMID:36007061<ref>PMID:36007061</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8d9e" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Candidatus Scalindua brodae]]
		+	[[Category: Large Structures]]
		+	[[Category: Hu C]]
		+	[[Category: Ke A]]
		+	[[Category: Nam KH]]
		+	[[Category: Schuler G]]

OCA: Protected "8d9e" [edit=sysop:move=sysop]

2022-06-15T04:16:40Z

Protected "8d9e" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:16, 15 June 2022

OCA: New page: '''Unreleased structure''' The entry 8d9e is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-15T04:16:39Z

New page: '''Unreleased structure''' The entry 8d9e is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8d9e is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8d9e - Revision history

OCA at 08:17, 14 June 2023

OCA: Protected "8d9e" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8d9e is ON HOLD Authors: Description: Category: Unreleased Structures