8dpq - Revision history

OCA at 10:12, 25 October 2023

2023-10-25T10:12:37Z

←Older revision		Revision as of 10:12, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8dpq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DPQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dpq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DPQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dpq OCA], [https://pdbe.org/8dpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dpq RCSB], [https://www.ebi.ac.uk/pdbsum/8dpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dpq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dpq OCA], [https://pdbe.org/8dpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dpq RCSB], [https://www.ebi.ac.uk/pdbsum/8dpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dpq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:29, 7 April 2023

2023-04-07T06:29:34Z

←Older revision		Revision as of 06:29, 7 April 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8dpq is ~~ON HOLD~~	+	==Beta-lactamase CTX-M-14 N170A==
		+	<StructureSection load='8dpq' size='340' side='right'caption='[[8dpq]], [[Resolution\|resolution]] 1.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dpq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DPQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dpq OCA], [https://pdbe.org/8dpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dpq RCSB], [https://www.ebi.ac.uk/pdbsum/8dpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dpq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H6UQI0_ECOLX H6UQI0_ECOLX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CTX-M beta-lactamases are a widespread source of resistance to beta-lactam antibiotics in Gram-negative bacteria. These enzymes readily hydrolyze penicillins and cephalosporins, including oxyimino-cephalosporins such as cefotaxime. To investigate the preference of CTX-M enzymes for cephalosporins, we examined eleven active-site residues in the CTX-M-14 beta-lactamase model system by alanine mutagenesis to assess the contribution of the residues to catalysis and specificity for the hydrolysis of the penicillin, ampicillin, and the cephalosporins cephalothin and cefotaxime. Key active site residues for class A beta-lactamases, including Lys73, Ser130, Asn132, Lys234, Thr216, and Thr235, contribute significantly to substrate binding and catalysis of penicillin and cephalosporin substrates in that alanine substitutions decrease both k(cat) and k(cat)/K(M). A second group of residues, including Asn104, Tyr105, Asn106, Thr215, and Thr216, contribute only to substrate binding, with the substitutions decreasing only k(cat)/K(M). Importantly, calculating the average effect of a substitution across the 11 active site residues shows that the most significant impact is on cefotaxime hydrolysis while ampicillin hydrolysis is least affected, suggesting the active site is highly optimized for cefotaxime catalysis. Furthermore, we determined X-ray crystal structures for the apo-enzymes of the mutants N106A, S130A, N132A, N170A, T215A, and T235A. Surprisingly, in the structures of some mutants, particularly N106A and T235A, the changes in structure propagate from the site of substitution to other regions of the active site, suggesting that the impact of substitutions is due to more widespread changes in structure and illustrating the interconnected nature of the active site.

-	~~Authors: Lu~~, S., ~~Neetu~~, N., ~~Palzkill~~, T.	+	Mutagenesis and structural analysis reveal the CTX-M beta-lactamase active site is optimized for cephalosporin catalysis and drug resistance.,Lu S, Montoya M, Hu L, Neetu N, Sankaran B, Prasad BVV, Palzkill T J Biol Chem. 2023 Mar 22:104630. doi: 10.1016/j.jbc.2023.104630. PMID:36963495<ref>PMID:36963495</ref>

-	~~Description: Beta~~-~~lactamase CTX~~-~~M-14 N170A~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Lu, S]]	+	<div class="pdbe-citations 8dpq" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Palzkill, T~~]]	+	== References ==
-	[[Category: ~~Neetu, N~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Lu S]]
		+	[[Category: Neetu N]]
		+	[[Category: Palzkill T]]

OCA at 05:41, 8 March 2023

2023-03-08T05:41:56Z

←Older revision		Revision as of 05:41, 8 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8dpq is ON HOLD ~~until Paper Publication~~	+	The entry 8dpq is ON HOLD

-	Authors: Lu, S., Palzkill, T.	+	Authors: Lu, S., Neetu, N., Palzkill, T.

	Description: Beta-lactamase CTX-M-14 N170A		Description: Beta-lactamase CTX-M-14 N170A
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	[[Category: Lu, S]]		[[Category: Lu, S]]
	[[Category: Palzkill, T]]		[[Category: Palzkill, T]]
		+	[[Category: Neetu, N]]

OCA at 03:48, 25 August 2022

2022-08-25T03:48:13Z

←Older revision		Revision as of 03:48, 25 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8dpq is ON HOLD	+	The entry 8dpq is ON HOLD until Paper Publication

	Authors: Lu, S., Palzkill, T.		Authors: Lu, S., Palzkill, T.

OCA: Protected "8dpq" [edit=sysop:move=sysop]

2022-07-27T16:45:20Z

Protected "8dpq" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:45, 27 July 2022

OCA: New page: '''Unreleased structure''' The entry 8dpq is ON HOLD Authors: Lu, S., Palzkill, T. Description: Beta-lactamase CTX-M-14 N170A Category: Unreleased Structures Category: Lu, S [[...

2022-07-27T16:45:19Z

New page: '''Unreleased structure''' The entry 8dpq is ON HOLD Authors: Lu, S., Palzkill, T. Description: Beta-lactamase CTX-M-14 N170A Category: Unreleased Structures Category: Lu, S [[...

New page

'''Unreleased structure'''

The entry 8dpq is ON HOLD

Authors: Lu, S., Palzkill, T.

Description: Beta-lactamase CTX-M-14 N170A
[[Category: Unreleased Structures]]
[[Category: Lu, S]]
[[Category: Palzkill, T]]