OCA at 10:03, 17 January 2024

2024-01-17T10:03:46Z

←Older revision		Revision as of 10:03, 17 January 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8dvw is ~~ON HOLD until Paper~~ Publication	+	==Structure of the Campylobacter concisus glycosyltransferase PglA R203Q==
		+	<StructureSection load='8dvw' size='340' side='right'caption='[[8dvw]], [[Resolution\|resolution]] 2.29Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dvw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_concisus Campylobacter concisus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DVW FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.29Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD2:URIDINE-DIPHOSPHATE-N-ACETYLGALACTOSAMINE'>UD2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dvw OCA], [https://pdbe.org/8dvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dvw RCSB], [https://www.ebi.ac.uk/pdbsum/8dvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dvw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0M4SVA9_9BACT A0A0M4SVA9_9BACT]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In selected Campylobacter species, the biosynthesis of N-linked glycoconjugates via the pgl pathway is essential for pathogenicity and survival. However, most of the membrane-associated GT-B fold glycosyltransferases responsible for diversifying glycans in this pathway have not been structurally characterized which hinders the understanding of the structural factors that govern substrate specificity and prediction of resulting glycan composition. Herein, we report the 1.8 A resolution structure of Campylobacter concisus PglA, the glycosyltransferase responsible for the transfer of N-acetylgalatosamine (GalNAc) from uridine 5'-diphospho-N-acetylgalactosamine (UDP-GalNAc) to undecaprenyl-diphospho-N,N'-diacetylbacillosamine (UndPP-diNAcBac) in complex with the sugar donor GalNAc. This study identifies distinguishing characteristics that set PglA apart within the GT4 enzyme family. Computational docking of the structure in the membrane in comparison to homologs points to differences in interactions with the membrane-embedded acceptor and the structural analysis of the complex together with bioinformatics and site-directed mutagenesis identifies donor sugar binding motifs. Notably, E113, conserved solely among PglA enzymes, forms a hydrogen bond with the GalNAc C6''-OH. Mutagenesis of E113 reveals activity consistent with this role in substrate binding, rather than stabilization of the oxocarbenium ion transition state, a function sometimes ascribed to the corresponding residue in GT4 homologs. The bioinformatic analyses reveal a substrate-specificity motif, showing that Pro281 in a substrate binding loop of PglA directs configurational preference for GalNAc over GlcNAc. This proline is replaced by a conformationally flexible glycine, even in distant homologs, which favor substrates with the same stereochemistry at C4, such as glucose. The signature loop is conserved across all Campylobacter PglA enzymes, emphasizing its importance in substrate specificity.

-	~~Authors~~:	+	Specificity determinants revealed by the structure of glycosyltransferase Campylobacter concisus PglA.,Vuksanovic N, Clasman JR, Imperiali B, Allen KN Protein Sci. 2024 Jan;33(1):e4848. doi: 10.1002/pro.4848. PMID:38019455<ref>PMID:38019455</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8dvw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Campylobacter concisus]]
		+	[[Category: Large Structures]]
		+	[[Category: Allen KN]]
		+	[[Category: Bernstein HM]]
		+	[[Category: Clasman JR]]
		+	[[Category: Imperiali B]]
		+	[[Category: Vuksanovic N]]

OCA at 15:30, 7 September 2022

2022-09-07T15:30:47Z

←Older revision		Revision as of 15:30, 7 September 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8dvw is ON HOLD	+	The entry 8dvw is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8dvw" [edit=sysop:move=sysop]

2022-08-10T04:03:54Z

Protected "8dvw" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:03, 10 August 2022

OCA: New page: '''Unreleased structure''' The entry 8dvw is ON HOLD Authors: Description: Category: Unreleased Structures

2022-08-10T04:03:53Z

New page: '''Unreleased structure''' The entry 8dvw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8dvw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8dvw - Revision history

OCA at 10:03, 17 January 2024

OCA at 15:30, 7 September 2022

OCA: Protected "8dvw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8dvw is ON HOLD Authors: Description: Category: Unreleased Structures