8e3g - Revision history

OCA at 10:17, 25 October 2023

2023-10-25T10:17:05Z

←Older revision		Revision as of 10:17, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8e3g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E3G FirstGlance]. <br>		<table><tr><td colspan='2'>[[8e3g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E3G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e3g OCA], [https://pdbe.org/8e3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e3g RCSB], [https://www.ebi.ac.uk/pdbsum/8e3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e3g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e3g OCA], [https://pdbe.org/8e3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e3g RCSB], [https://www.ebi.ac.uk/pdbsum/8e3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e3g ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 8e3g" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 8e3g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Growth differentiation factor 3D STRUCTURES\|Growth differentiation factor 3D STRUCTURES]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:28, 15 February 2023

2023-02-15T10:28:20Z

←Older revision		Revision as of 10:28, 15 February 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8e3g is ~~ON HOLD~~	+	==BMP2/GDF5 heterodimer==
		+	<StructureSection load='8e3g' size='340' side='right'caption='[[8e3g]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8e3g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E3G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e3g OCA], [https://pdbe.org/8e3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e3g RCSB], [https://www.ebi.ac.uk/pdbsum/8e3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e3g ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BMP2_HUMAN BMP2_HUMAN] Induces cartilage and bone formation.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Proteins of the TGFbeta family, which are largely studied as homodimers, are also known to form heterodimers with biological activity distinct from their component homodimers. For instance, heterodimers of bone morphogenetic proteins, including BMP2/BMP7, BMP2/BMP6, and BMP9/BMP10, among others, have illustrated the importance of these heterodimeric proteins within the context of TGFbeta signaling. RESULTS: In this study, we have determined that mature GDF5 can be combined with mature BMP2 or BMP4 to form BMP2/GDF5 and BMP4/GDF5 heterodimer. Intriguingly, this combination of a BMP2 or BMP4 monomer, which exhibit high affinity to heparan sulfate characteristic to the BMP class, with a GDF5 monomer with low heparan sulfate affinity produces a heterodimer with an intermediate affinity. Using heparin affinity chromatography to purify the heterodimeric proteins, we then determined that both the BMP2/GDF5 and BMP4/GDF5 heterodimers consistently signaled potently across an array of cellular and in vivo systems, while the activities of their homodimeric counterparts were more context dependent. These differences were likely driven by an increase in the combined affinities for the type 1 receptors, Alk3 and Alk6. Furthermore, the X-ray crystal structure of BMP2/GDF5 heterodimer was determined, highlighting the formation of two asymmetric type 1 receptor binding sites that are both unique relative to the homodimers. CONCLUSIONS: Ultimately, this method of heterodimer production yielded a signaling molecule with unique properties relative to the homodimeric ligands, including high affinity to multiple type 1 and moderate heparan binding affinity.

-	~~Authors~~:	+	Formation and characterization of BMP2/GDF5 and BMP4/GDF5 heterodimers.,Gipson GR, Nolan K, Kattamuri C, Kenny AP, Agricola Z, Edwards NA, Zinski J, Czepnik M, Mullins MC, Zorn AM, Thompson TB BMC Biol. 2023 Feb 1;21(1):16. doi: 10.1186/s12915-023-01522-4. PMID:36726183<ref>PMID:36726183</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8e3g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Gipson GR]]
		+	[[Category: Nolan KT]]
		+	[[Category: Thompson TB]]

OCA: Protected "8e3g" [edit=sysop:move=sysop]

2022-08-25T03:52:53Z

Protected "8e3g" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:52, 25 August 2022

OCA: New page: '''Unreleased structure''' The entry 8e3g is ON HOLD Authors: Description: Category: Unreleased Structures

2022-08-25T03:52:52Z

New page: '''Unreleased structure''' The entry 8e3g is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8e3g is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]