OCA at 07:33, 18 January 2023

2023-01-18T07:33:15Z

←Older revision		Revision as of 07:33, 18 January 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8e3q is ~~ON HOLD~~	+	==CRYO-EM STRUCTURE OF the human MPSF==
		+	<StructureSection load='8e3q' size='340' side='right'caption='[[8e3q]], [[Resolution\|resolution]] 2.68Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8e3q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E3Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E3Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e3q OCA], [https://pdbe.org/8e3q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e3q RCSB], [https://www.ebi.ac.uk/pdbsum/8e3q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e3q ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The polyadenylation signal (PAS) is a key sequence element for 3'-end cleavage and polyadenylation of messenger RNA precursors (pre-mRNAs). This hexanucleotide motif is recognized by the mammalian polyadenylation specificity factor (mPSF), consisting of CPSF160, WDR33, CPSF30, and Fip1 subunits. Recent studies have revealed how the AAUAAA PAS, the most frequently observed PAS, is recognized by mPSF. We report here the structure of human mPSF in complex with the AUUAAA PAS, the second most frequently identified PAS. Conformational differences are observed for the A1 and U2 nucleotides in AUUAAA compared to the A1 and A2 nucleotides in AAUAAA, while the binding modes of the remaining 4 nt are essentially identical. The 5' phosphate of U2 moves by 2.6 A and the U2 base is placed near the six-membered ring of A2 in AAUAAA, where it makes two hydrogen bonds with zinc finger 2 (ZF2) of CPSF30, which undergoes conformational changes as well. We also attempted to determine the binding modes of two rare PAS hexamers, AAGAAA and GAUAAA, but did not observe the RNA in the cryo-electron microscopy density. The residues in CPSF30 (ZF2 and ZF3) and WDR33 that recognize PAS are disordered in these two structures.

-	~~Authors: Gutierrez, P.A~~., ~~Wei~~, J., Sun, Y., Tong, L.	+	Molecular basis for the recognition of the AUUAAA polyadenylation signal by mPSF.,Gutierrez PA, Wei J, Sun Y, Tong L RNA. 2022 Nov;28(11):1534-1541. doi: 10.1261/rna.079322.122. Epub 2022 Sep 6. PMID:36130077<ref>PMID:36130077</ref>

-	~~Description: CRYO-EM STRUCTURE OF~~ the ~~human MPSF~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Tong, L~~]]	+	<div class="pdbe-citations 8e3q" style="background-color:#fffaf0;"></div>
-	[[Category: Gutierrez~~, P.A~~]]	+	== References ==
-	[[Category: ~~Wei, J~~]]	+	<references/>
-	[[Category: ~~Sun, Y~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Gutierrez PA]]
		+	[[Category: Sun Y]]
		+	[[Category: Tong L]]
		+	[[Category: Wei J]]

OCA at 05:45, 11 January 2023

2023-01-11T05:45:48Z

←Older revision		Revision as of 05:45, 11 January 2023
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	Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L.		Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L.

-	Description: ~~CryoEM structure of mPSF in complex with the non~~-~~canonical PAS AAGAAA (Not observed)~~	+	Description: CRYO-EM STRUCTURE OF the human MPSF
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Tong, L]]		[[Category: Tong, L]]

OCA: Protected "8e3q" [edit=sysop:move=sysop]

2022-08-25T03:53:17Z

Protected "8e3q" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:53, 25 August 2022

OCA: New page: '''Unreleased structure''' The entry 8e3q is ON HOLD Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L. Description: CryoEM structure of mPSF in complex with the non-canonical PAS AA...

2022-08-25T03:53:16Z

New page: '''Unreleased structure''' The entry 8e3q is ON HOLD Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L. Description: CryoEM structure of mPSF in complex with the non-canonical PAS AA...

New page

'''Unreleased structure'''

The entry 8e3q is ON HOLD

Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L.

Description: CryoEM structure of mPSF in complex with the non-canonical PAS AAGAAA (Not observed)
[[Category: Unreleased Structures]]
[[Category: Tong, L]]
[[Category: Gutierrez, P.A]]
[[Category: Wei, J]]
[[Category: Sun, Y]]

8e3q - Revision history

OCA at 07:33, 18 January 2023

OCA at 05:45, 11 January 2023

OCA: Protected "8e3q" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8e3q is ON HOLD Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L. Description: CryoEM structure of mPSF in complex with the non-canonical PAS AA...