8ee0 - Revision history

OCA at 10:20, 25 October 2023

2023-10-25T10:20:47Z

←Older revision		Revision as of 10:20, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8ee0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EE0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ee0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EE0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ee0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ee0 OCA], [https://pdbe.org/8ee0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ee0 RCSB], [https://www.ebi.ac.uk/pdbsum/8ee0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ee0 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ee0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ee0 OCA], [https://pdbe.org/8ee0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ee0 RCSB], [https://www.ebi.ac.uk/pdbsum/8ee0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ee0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 05:42, 31 May 2023

2023-05-31T05:42:34Z

←Older revision		Revision as of 05:42, 31 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8ee0 is ~~ON HOLD until Paper~~ Publication	+	==KS-AT didomain from module 2 of the 6-deoxyerythronolide B synthase in complex with antibody fragment 1B2==
		+	<StructureSection load='8ee0' size='340' side='right'caption='[[8ee0]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ee0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EE0 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ee0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ee0 OCA], [https://pdbe.org/8ee0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ee0 RCSB], [https://www.ebi.ac.uk/pdbsum/8ee0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ee0 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5UNP6_SACER Q5UNP6_SACER]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fragment antigen-binding domains of antibodies (F(ab)s) are powerful probes of structure-function relationships of assembly line polyketide synthases (PKSs). We report the discovery and characterization of F(ab)s interrogating the structure and function of the ketosynthase-acyltransferase (KS-AT) core of Module 2 of the 6-deoxyerythronolide B synthase (DEBS). Two F(ab)s (AC2 and BB1) were identified to potently inhibit the catalytic activity of Module 2. Both AC2 and BB1 were found to modulate ACP-mediated reactions catalyzed by this module, albeit by distinct mechanisms. AC2 primarily affects the rate (k(cat)), whereas BB1 increases the K(M) of an ACP-mediated reaction. A third F(ab), AA5, binds to the KS-AT fragment of DEBS Module 2 without altering either parameter; it is phenotypically reminiscent of a previously characterized F(ab), 1B2, shown to principally recognize the N-terminal helical docking domain of DEBS Module 3. Crystal structures of AA5 and 1B2 bound to the KS-AT fragment of Module 2 were solved to 2.70 and 2.65 A resolution, respectively, and revealed entirely distinct recognition features of the two antibodies. The new tools and insights reported here pave the way toward advancing our understanding of the structure-function relationships of DEBS Module 2, arguably the most well-studied module of an assembly line PKS.

-	~~Authors: Cogan, D.P~~., Brodsky, ~~K.L.~~, ~~Guzman~~, ~~K.M.~~, Mathews, ~~I.I.~~, ~~Khosla~~, C.	+	Discovery and Characterization of Antibody Probes of Module 2 of the 6-Deoxyerythronolide B Synthase.,Guzman KM, Cogan DP, Brodsky KL, Soohoo AM, Li X, Sevillano N, Mathews II, Nguyen KP, Craik CS, Khosla C Biochemistry. 2023 May 15. doi: 10.1021/acs.biochem.3c00156. PMID:37184546<ref>PMID:37184546</ref>

-	~~Description: KS-AT didomain from module 2~~ of the 6-~~deoxyerythronolide B synthase in complex with antibody fragment 1B2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Mathews, I.I~~]]	+	<div class="pdbe-citations 8ee0" style="background-color:#fffaf0;"></div>
-	[[Category: Brodsky~~, K.L~~]]	+	== References ==
-	[[Category: Guzman~~, K.M~~]]	+	<references/>
-	[[Category: Khosla, C]]	+	__TOC__
-	[[Category: ~~Cogan, D.P~~]]	+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Saccharopolyspora erythraea]]
		+	[[Category: Brodsky KL]]
		+	[[Category: Cogan DP]]
		+	[[Category: Guzman KM]]
		+	[[Category: Khosla C]]
		+	[[Category: Mathews II]]

OCA at 17:09, 19 October 2022

2022-10-19T17:09:27Z

←Older revision		Revision as of 17:09, 19 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ee0 is ON HOLD	+	The entry 8ee0 is ON HOLD until Paper Publication

	Authors: Cogan, D.P., Brodsky, K.L., Guzman, K.M., Mathews, I.I., Khosla, C.		Authors: Cogan, D.P., Brodsky, K.L., Guzman, K.M., Mathews, I.I., Khosla, C.

OCA: Protected "8ee0" [edit=sysop:move=sysop]

2022-09-14T05:49:44Z

Protected "8ee0" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 14 September 2022

OCA: New page: '''Unreleased structure''' The entry 8ee0 is ON HOLD Authors: Cogan, D.P., Brodsky, K.L., Guzman, K.M., Mathews, I.I., Khosla, C. Description: KS-AT didomain from module 2 of the 6-deo...

2022-09-14T05:49:43Z

New page: '''Unreleased structure''' The entry 8ee0 is ON HOLD Authors: Cogan, D.P., Brodsky, K.L., Guzman, K.M., Mathews, I.I., Khosla, C. Description: KS-AT didomain from module 2 of the 6-deo...

New page

'''Unreleased structure'''

The entry 8ee0 is ON HOLD

Authors: Cogan, D.P., Brodsky, K.L., Guzman, K.M., Mathews, I.I., Khosla, C.

Description: KS-AT didomain from module 2 of the 6-deoxyerythronolide B synthase in complex with antibody fragment 1B2
[[Category: Unreleased Structures]]
[[Category: Mathews, I.I]]
[[Category: Brodsky, K.L]]
[[Category: Guzman, K.M]]
[[Category: Khosla, C]]
[[Category: Cogan, D.P]]