8ejy - Revision history

OCA at 10:21, 25 October 2023

2023-10-25T10:21:54Z

←Older revision		Revision as of 10:21, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8ejy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planobispora_rosea Planobispora rosea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EJY FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ejy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planobispora_rosea Planobispora rosea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EJY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ejy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ejy OCA], [https://pdbe.org/8ejy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ejy RCSB], [https://www.ebi.ac.uk/pdbsum/8ejy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ejy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.47Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ejy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ejy OCA], [https://pdbe.org/8ejy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ejy RCSB], [https://www.ebi.ac.uk/pdbsum/8ejy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ejy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 10:15, 24 November 2022

2022-11-24T10:15:56Z

←Older revision		Revision as of 10:15, 24 November 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8ejy is ~~ON HOLD until Paper~~ Publication	+	==[4+2] Aza-Cyclase F293A variant==
		+	<StructureSection load='8ejy' size='340' side='right'caption='[[8ejy]], [[Resolution\|resolution]] 2.47Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ejy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planobispora_rosea Planobispora rosea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EJY FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ejy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ejy OCA], [https://pdbe.org/8ejy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ejy RCSB], [https://www.ebi.ac.uk/pdbsum/8ejy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ejy ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/U5Q3T2_PLARO U5Q3T2_PLARO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thiazole-containing pyritides (thiopeptides) are ribosomally synthesized and post-translationally modified peptides (RiPPs) that have attracted interest owing to their potent biological activities and structural complexity. The class-defining feature of a thiopeptide is a six-membered, nitrogenous heterocycle formed by an enzymatic [4 + 2]-cycloaddition. In rare cases, piperidine or dehydropiperidine (DHP) is present; however, the aromatized pyridine is considerably more common. Despite significant effort, the mechanism by which the central pyridine is formed remains poorly understood. Building on our recent observation of the Bycroft-Gowland intermediate (i.e., the direct product of the [4 + 2]-cycloaddition), we interrogated thiopeptide pyridine synthases using a combination of targeted mutagenesis, kinetic assays, substrate analogs, enzyme-substrate cross-linking, and chemical rescue experiments. Collectively, our data delineate roles for several conserved residues in thiopeptide pyridine synthases. A critical tyrosine facilitates the final aromatization step of pyridine formation. This work provides a foundation for further exploration of the [4 + 2]-cycloaddition reaction and future customization of pyridine-containing macrocyclic peptides.

-	~~Authors:~~ Nair, S.K.	+	Enzymatic Pyridine Aromatization during Thiopeptide Biosynthesis.,Rice AJ, Pelton JM, Kramer NJ, Catlin DS, Nair SK, Pogorelov TV, Mitchell DA, Bowers AA J Am Chem Soc. 2022 Nov 23;144(46):21116-21124. doi: 10.1021/jacs.2c07377. Epub , 2022 Nov 9. PMID:36351243<ref>PMID:36351243</ref>

-	~~Description~~: [~~4+2~~] ~~Aza-Cyclase F293A variant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Nair~~, S.K~~]]	+	<div class="pdbe-citations 8ejy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Planobispora rosea]]
		+	[[Category: Nair SK]]

OCA at 03:59, 26 October 2022

2022-10-26T03:59:06Z

←Older revision		Revision as of 03:59, 26 October 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ejy is ON HOLD	+	The entry 8ejy is ON HOLD until Paper Publication

	Authors: Nair, S.K.		Authors: Nair, S.K.

OCA: Protected "8ejy" [edit=sysop:move=sysop]

2022-09-28T04:46:08Z

Protected "8ejy" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:46, 28 September 2022

OCA: New page: '''Unreleased structure''' The entry 8ejy is ON HOLD Authors: Nair, S.K. Description: [4+2] Aza-Cyclase F293A variant Category: Unreleased Structures Category: Nair, S.K

2022-09-28T04:46:07Z

New page: '''Unreleased structure''' The entry 8ejy is ON HOLD Authors: Nair, S.K. Description: [4+2] Aza-Cyclase F293A variant Category: Unreleased Structures Category: Nair, S.K

New page

'''Unreleased structure'''

The entry 8ejy is ON HOLD

Authors: Nair, S.K.

Description: [4+2] Aza-Cyclase F293A variant
[[Category: Unreleased Structures]]
[[Category: Nair, S.K]]