OCA at 04:04, 25 May 2023

2023-05-25T04:04:44Z

←Older revision		Revision as of 04:04, 25 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8emx is ~~ON HOLD until Paper~~ Publication	+	==Phospholipase C beta 3 (PLCb3) in complex with Gbg on lipid nanodiscs==
		+	<StructureSection load='8emx' size='340' side='right'caption='[[8emx]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8emx]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EMX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8emx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8emx OCA], [https://pdbe.org/8emx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8emx RCSB], [https://www.ebi.ac.uk/pdbsum/8emx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8emx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PLCB3_HUMAN PLCB3_HUMAN] The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phospholipase C-betas (PLCbetas) catalyze the hydrolysis of phosphatidylinositol 4, 5-bisphosphate [Formula: see text] into [Formula: see text] [Formula: see text] and [Formula: see text] [Formula: see text]. [Formula: see text] regulates the activity of many membrane proteins, while IP3 and DAG lead to increased intracellular Ca(2+) levels and activate protein kinase C, respectively. PLCbetas are regulated by G protein-coupled receptors through direct interaction with [Formula: see text] and [Formula: see text] and are aqueous-soluble enzymes that must bind to the cell membrane to act on their lipid substrate. This study addresses the mechanism by which [Formula: see text] activates PLCbeta3. We show that PLCbeta3 functions as a slow Michaelis-Menten enzyme ( [Formula: see text] ) on membrane surfaces. We used membrane partitioning experiments to study the solution-membrane localization equilibrium of PLCbeta3. Its partition coefficient is such that only a small quantity of PLCbeta3 exists in the membrane in the absence of [Formula: see text] . When [Formula: see text] is present, equilibrium binding on the membrane surface increases PLCbeta3 in the membrane, increasing [Formula: see text] in proportion. Atomic structures on membrane vesicle surfaces show that two [Formula: see text] anchor PLCbeta3 with its catalytic site oriented toward the membrane surface. Taken together, the enzyme kinetic, membrane partitioning, and structural data show that [Formula: see text] activates PLCbeta by increasing its concentration on the membrane surface and orienting its catalytic core to engage [Formula: see text] . This principle of activation explains rapid stimulated catalysis with low background activity, which is essential to the biological processes mediated by [Formula: see text], IP3, and DAG.

-	~~Authors~~:	+	Gbetagamma activates PIP2 hydrolysis by recruiting and orienting PLCbeta on the membrane surface.,Falzone ME, MacKinnon R Proc Natl Acad Sci U S A. 2023 May 16;120(20):e2301121120. doi: , 10.1073/pnas.2301121120. Epub 2023 May 12. PMID:37172014<ref>PMID:37172014</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8emx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Falzone ME]]
		+	[[Category: MacKinnon R]]

OCA: Protected "8emx" [edit=sysop:move=sysop]

2022-10-19T17:15:37Z

Protected "8emx" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:15, 19 October 2022

OCA: New page: '''Unreleased structure''' The entry 8emx is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2022-10-19T17:15:36Z

New page: '''Unreleased structure''' The entry 8emx is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8emx is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

8emx - Revision history

OCA at 04:04, 25 May 2023

OCA: Protected "8emx" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8emx is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures