8evc - Revision history

OCA at 05:27, 9 August 2023

2023-08-09T05:27:03Z

←Older revision		Revision as of 05:27, 9 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8evc is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open state==
		+	<StructureSection load='8evc' size='340' side='right'caption='[[8evc]], [[Resolution\|resolution]] 3.33Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8evc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EVC FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.33Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCG:CYCLIC+GUANOSINE+MONOPHOSPHATE'>PCG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8evc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8evc OCA], [https://pdbe.org/8evc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8evc RCSB], [https://www.ebi.ac.uk/pdbsum/8evc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8evc ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CNGA3_HUMAN CNGA3_HUMAN] Achromatopsia;Cone rod dystrophy. The disease is caused by mutations affecting the gene represented in this entry. Defects in CNGA3 may be a cause of Leber congenital amaurosis (LCA), a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CNGA3_HUMAN CNGA3_HUMAN] Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3 (By similarity). Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones.<ref>PMID:10888875</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cyclic nucleotide-gated (CNG) channels transduce chemical signals into electrical signals in sensory receptors and neurons. They are activated by cGMP or cAMP, which bind to the cyclic nucleotide-binding domain (CNBD) to open a gate located 50-60 A away in the central cavity. Structures of closed and open vertebrate CNG channels have been solved, but the conformational landscape of this allosteric gating remains to be elucidated and enriched. Here, we report structures of the cGMP-activated human cone photoreceptor CNGA3/CNGB3 channel in closed, intermediate, pre-open and open states in detergent or lipid nanodisc, all with fully bound cGMP. The pre-open and open states are obtained only in the lipid nanodisc, suggesting a critical role of lipids in tuning the energetic landscape of CNGA3/CNGB3 activation. The different states exhibit subunit-unique, incremental and distinct conformational rearrangements that originate in the CNBD, propagate through the gating ring to the transmembrane domain, and gradually open the S6 cavity gate. Our work illustrates a spatial conformational-change wave of allosteric gating of a vertebrate CNG channel by its natural ligand and provides an expanded framework for studying CNG properties and channelopathy.

-	~~Authors: Hu~~, Z., ~~Yang~~, J.	+	Conformational trajectory of allosteric gating of the human cone photoreceptor cyclic nucleotide-gated channel.,Hu Z, Zheng X, Yang J Nat Commun. 2023 Jul 18;14(1):4284. doi: 10.1038/s41467-023-39971-8. PMID:37463923<ref>PMID:37463923</ref>

-	~~Description: Cryo-EM structure of cGMP bound truncated human CNGA3~~/~~CNGB3 channel in lipid nanodisc~~, ~~open state~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Yang, J~~]]	+	<div class="pdbe-citations 8evc" style="background-color:#fffaf0;"></div>
-	[[Category: Hu, Z]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Hu Z]]
		+	[[Category: Yang J]]

OCA at 07:47, 19 April 2023

2023-04-19T07:47:07Z

←Older revision		Revision as of 07:47, 19 April 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8evc is ON HOLD until ~~2024-04-20~~	+	The entry 8evc is ON HOLD until Paper Publication

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.

OCA at 05:00, 18 January 2023

2023-01-18T05:00:15Z

←Older revision		Revision as of 05:00, 18 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8evc is ON HOLD	+	The entry 8evc is ON HOLD until 2024-04-20

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.
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	Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open state		Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Hu, Z]]
	[[Category: Yang, J]]		[[Category: Yang, J]]
		+	[[Category: Hu, Z]]

OCA at 07:34, 14 December 2022

2022-12-14T07:34:05Z

←Older revision		Revision as of 07:34, 14 December 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8evc is ON HOLD ~~until 2024-04-20~~	+	The entry 8evc is ON HOLD

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.
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	Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open state		Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Yang, J]]
	[[Category: Hu, Z]]		[[Category: Hu, Z]]
		+	[[Category: Yang, J]]

OCA at 08:08, 24 November 2022

2022-11-24T08:08:36Z

←Older revision		Revision as of 08:08, 24 November 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8evc is ON HOLD	+	The entry 8evc is ON HOLD until 2024-04-20

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.

OCA: Protected "8evc" [edit=sysop:move=sysop]

2022-10-26T04:07:12Z

Protected "8evc" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 26 October 2022

OCA: New page: '''Unreleased structure''' The entry 8evc is ON HOLD Authors: Hu, Z., Yang, J. Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open ...

2022-10-26T04:07:11Z

New page: '''Unreleased structure''' The entry 8evc is ON HOLD Authors: Hu, Z., Yang, J. Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open ...

New page

'''Unreleased structure'''

The entry 8evc is ON HOLD

Authors: Hu, Z., Yang, J.

Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open state
[[Category: Unreleased Structures]]
[[Category: Yang, J]]
[[Category: Hu, Z]]