http://52.214.119.220/wiki/index.php?action=history&feed=atom&title=8fr7 8fr7 - Revision history 2026-04-04T13:33:37Z Revision history for this page on the wiki MediaWiki 1.11.2 http://52.214.119.220/wiki/index.php?title=8fr7&diff=3889699&oldid=prev OCA at 12:32, 4 October 2023 2023-10-04T12:32:27Z <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:32, 4 October 2023</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Unreleased structure'''</div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The entry </del>8fr7 <del style="color: red; font-weight: bold; text-decoration: none;">is ON HOLD until Paper Publication</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==A hinge glycan regulates spike bending and impacts coronavirus infectivity==</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;StructureSection load='</ins>8fr7<ins style="color: red; font-weight: bold; text-decoration: none;">' size='340' side='right'caption='[[8fr7]], [[Resolution|resolution]] 3.39&amp;Aring;' scene=''&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Authors</del>: <del style="color: red; font-weight: bold; text-decoration: none;">Pintilie</del>, <del style="color: red; font-weight: bold; text-decoration: none;">G</del>., <del style="color: red; font-weight: bold; text-decoration: none;">Wilson</del>, <del style="color: red; font-weight: bold; text-decoration: none;">E.</del>, <del style="color: red; font-weight: bold; text-decoration: none;">Chmielewski</del>, D., <del style="color: red; font-weight: bold; text-decoration: none;">Schmid, M</del>.<del style="color: red; font-weight: bold; text-decoration: none;">F</del>., <del style="color: red; font-weight: bold; text-decoration: none;">Jin</del>, <del style="color: red; font-weight: bold; text-decoration: none;">J</del>., <del style="color: red; font-weight: bold; text-decoration: none;">Chen</del>, <del style="color: red; font-weight: bold; text-decoration: none;">M</del>., <del style="color: red; font-weight: bold; text-decoration: none;">Singharoy</del>, <del style="color: red; font-weight: bold; text-decoration: none;">A</del>., <del style="color: red; font-weight: bold; text-decoration: none;">Chiu</del>, <del style="color: red; font-weight: bold; text-decoration: none;">W</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== Structural highlights ==</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;table&gt;&lt;tr&gt;&lt;td colspan='2'&gt;[[8fr7]] is a 3 chain structure with sequence from [https</ins>:<ins style="color: red; font-weight: bold; text-decoration: none;">//en.wikipedia.org/wiki/Human_coronavirus_NL63 Human coronavirus NL63]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FR7 OCA]. For a &lt;b&gt;guided tour on the structure components&lt;/b&gt; use [https://proteopedia.org/fgij/fg.htm?mol=8FR7 FirstGlance]. &lt;br&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Description: A hinge glycan regulates spike bending and impacts coronavirus infectivity</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;/td&gt;&lt;/tr&gt;&lt;tr id='method'&gt;&lt;td class=&quot;sblockLbl&quot;&gt;&lt;b&gt;[[Empirical_models|Method:]]&lt;/b&gt;&lt;/td&gt;&lt;td class=&quot;sblockDat&quot; id=&quot;methodDat&quot;&gt;Electron Microscopy</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">[[Resolution|Resolution]] 3</ins>.<ins style="color: red; font-weight: bold; text-decoration: none;">39&amp;#8491;&lt;/td&gt;&lt;/tr&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: <del style="color: red; font-weight: bold; text-decoration: none;">Unreleased Structures</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;tr id='ligand'&gt;&lt;td class=&quot;sblockLbl&quot;&gt;&lt;b&gt;[[Ligand|Ligands:]]&lt;/b&gt;&lt;/td&gt;&lt;td class=&quot;sblockDat&quot; id=&quot;ligandDat&quot;&gt;&lt;scene name='pdbligand=BMA:BETA-D-MANNOSE'&gt;BMA&lt;/scene&gt;</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">&lt;scene name='pdbligand=FUC:ALPHA-L-FUCOSE'&gt;FUC&lt;/scene&gt;</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">&lt;scene name='pdbligand=GAL:BETA-D-GALACTOSE'&gt;GAL&lt;/scene&gt;</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">&lt;scene name='pdbligand=MAN:ALPHA-D-MANNOSE'&gt;MAN&lt;/scene&gt;</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">&lt;scene name='pdbligand=NAG:N-ACETYL-</ins>D<ins style="color: red; font-weight: bold; text-decoration: none;">-GLUCOSAMINE'&gt;NAG&lt;/scene&gt;&lt;/td&gt;&lt;/tr&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: <del style="color: red; font-weight: bold; text-decoration: none;">Chmielewski, D</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;tr id='resources'&gt;&lt;td class=&quot;sblockLbl&quot;&gt;&lt;b&gt;Resources:&lt;/b&gt;&lt;/td&gt;&lt;td class=&quot;sblockDat&quot;&gt;&lt;span class='plainlinks'&gt;[https://proteopedia</ins>.<ins style="color: red; font-weight: bold; text-decoration: none;">org/fgij/fg.htm?mol=8fr7 FirstGlance]</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">[http://oca</ins>.<ins style="color: red; font-weight: bold; text-decoration: none;">weizmann</ins>.<ins style="color: red; font-weight: bold; text-decoration: none;">ac.il/oca-bin/ocaids?id=8fr7 OCA]</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">[https://pdbe.org/8fr7 PDBe]</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">[https://www</ins>.<ins style="color: red; font-weight: bold; text-decoration: none;">rcsb.org/pdb/explore.do?structureId=8fr7 RCSB]</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">[https://www.ebi.ac.uk/pdbsum/8fr7 PDBsum]</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">[https://prosat</ins>.<ins style="color: red; font-weight: bold; text-decoration: none;">h-its.org/prosat/prosatexe?pdbcode=8fr7 ProSAT]&lt;/span&gt;&lt;/td&gt;&lt;/tr&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: <del style="color: red; font-weight: bold; text-decoration: none;">Wilson, E</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;/table&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: Chiu<del style="color: red; font-weight: bold; text-decoration: none;">, </del>W]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== Function ==</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: <del style="color: red; font-weight: bold; text-decoration: none;">Singharoy, A</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[https://www.uniprot.org/uniprot/SPIKE_CVHNL SPIKE_CVHNL] S1 region attaches the virion to the cell membrane by interacting with human ACE2</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state</ins>. <ins style="color: red; font-weight: bold; text-decoration: none;">During viral and target cell membrane fusion</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure</ins>, <ins style="color: red; font-weight: bold; text-decoration: none;">positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity)</ins>.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: <del style="color: red; font-weight: bold; text-decoration: none;">Chen, M</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">__TOC__</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: Pintilie<del style="color: red; font-weight: bold; text-decoration: none;">, </del>G]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;/StructureSection&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: Schmid<del style="color: red; font-weight: bold; text-decoration: none;">, M.F</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: <ins style="color: red; font-weight: bold; text-decoration: none;">Human coronavirus NL63</ins>]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: <del style="color: red; font-weight: bold; text-decoration: none;">Jin, J</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: <ins style="color: red; font-weight: bold; text-decoration: none;">Large Structures</ins>]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: <ins style="color: red; font-weight: bold; text-decoration: none;">Chen M</ins>]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: Chiu W]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: <ins style="color: red; font-weight: bold; text-decoration: none;">Chmielewski D</ins>]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: <ins style="color: red; font-weight: bold; text-decoration: none;">Jin J</ins>]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: Pintilie G]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: Schmid <ins style="color: red; font-weight: bold; text-decoration: none;">MF</ins>]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: <ins style="color: red; font-weight: bold; text-decoration: none;">Singharoy A]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category: Wilson E</ins>]]</div></td></tr> </table> OCA http://52.214.119.220/wiki/index.php?title=8fr7&diff=3714844&oldid=prev OCA at 08:42, 15 February 2023 2023-02-15T08:42:42Z <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 08:42, 15 February 2023</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Unreleased structure'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Unreleased structure'''</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The entry 8fr7 is ON HOLD </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The entry 8fr7 is ON HOLD <ins style="color: red; font-weight: bold; text-decoration: none;"> until Paper Publication</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Authors: Pintilie, G., Wilson, E., Chmielewski, D., Schmid, M.F., Jin, J., Chen, M., Singharoy, A., Chiu, W.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Authors: Pintilie, G., Wilson, E., Chmielewski, D., Schmid, M.F., Jin, J., Chen, M., Singharoy, A., Chiu, W.</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Unreleased Structures]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Unreleased Structures]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Chmielewski, D]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Chmielewski, D]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category: Wilson, E]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Chiu, W]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Chiu, W]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Singharoy, A]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Singharoy, A]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Chen, M]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Chen, M]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category: Wilson, E]]</div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Pintilie, G]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Pintilie, G]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Schmid, M.F]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Schmid, M.F]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Jin, J]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category: Jin, J]]</div></td></tr> </table> OCA http://52.214.119.220/wiki/index.php?title=8fr7&diff=3698050&oldid=prev OCA: Protected "8fr7" [edit=sysop:move=sysop] 2023-01-18T05:10:29Z <p>Protected &quot;<a href="/wiki/index.php/8fr7" title="8fr7">8fr7</a>&quot; [edit=sysop:move=sysop]</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 05:10, 18 January 2023</td> </tr> </table> OCA http://52.214.119.220/wiki/index.php?title=8fr7&diff=3698049&oldid=prev OCA: New page: '''Unreleased structure''' The entry 8fr7 is ON HOLD Authors: Pintilie, G., Wilson, E., Chmielewski, D., Schmid, M.F., Jin, J., Chen, M., Singharoy, A., Chiu, W. Description: A hinge g... 2023-01-18T05:10:27Z <p>New page: '''Unreleased structure''' The entry 8fr7 is ON HOLD Authors: Pintilie, G., Wilson, E., Chmielewski, D., Schmid, M.F., Jin, J., Chen, M., Singharoy, A., Chiu, W. Description: A hinge g...</p> <p><b>New page</b></p><div>'''Unreleased structure'''<br /> <br /> The entry 8fr7 is ON HOLD <br /> <br /> Authors: Pintilie, G., Wilson, E., Chmielewski, D., Schmid, M.F., Jin, J., Chen, M., Singharoy, A., Chiu, W.<br /> <br /> Description: A hinge glycan regulates spike bending and impacts coronavirus infectivity<br /> [[Category: Unreleased Structures]]<br /> [[Category: Chmielewski, D]]<br /> [[Category: Chiu, W]]<br /> [[Category: Singharoy, A]]<br /> [[Category: Chen, M]]<br /> [[Category: Wilson, E]]<br /> [[Category: Pintilie, G]]<br /> [[Category: Schmid, M.F]]<br /> [[Category: Jin, J]]</div> OCA