8fru - Revision history

OCA at 05:33, 17 April 2024

2024-04-17T05:33:51Z

←Older revision		Revision as of 05:33, 17 April 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/V6TF11_GIAIN V6TF11_GIAIN]		[https://www.uniprot.org/uniprot/V6TF11_GIAIN V6TF11_GIAIN]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure of the G. lamblia 80S ribosome bound to tRNA, mRNA, and the antibiotic emetine by cryo-electron microscopy, to an overall resolution of 2.49 A. The structure reveals rapidly evolving protein and nucleotide regions, differences in the peptide exit tunnel, and likely altered ribosome quality control pathways. Examination of translation initiation factor binding sites suggests these interactions are conserved despite a divergent initiation mechanism. Highlighting the potential of G. lamblia to resolve conserved biological principles; our structure reveals the interactions of the translation inhibitor emetine with the ribosome and mRNA, thus providing insight into the mechanism of action for this widely used antibiotic. Our work defines key questions in G. lamblia and motivates future experiments to explore the diversity of eukaryotic gene regulation.
-
-	The Giardia lamblia ribosome structure reveals divergence in several biological pathways and the mode of emetine function.,Eiler DR, Wimberly BT, Bilodeau DY, Taliaferro JM, Reigan P, Rissland OS, Kieft JS Structure. 2024 Jan 11:S0969-2126(23)00479-3. doi: 10.1016/j.str.2023.12.015. PMID:38242118<ref>PMID:38242118</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8fru" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:41, 1 February 2024

2024-02-01T11:41:33Z

←Older revision		Revision as of 11:41, 1 February 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8fru is ~~ON HOLD until Paper~~ Publication	+	==60S subunit of the Giardia lamblia 80S ribosome==
		+	<StructureSection load='8fru' size='340' side='right'caption='[[8fru]], [[Resolution\|resolution]] 2.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fru]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis_assemblage_A Giardia intestinalis assemblage A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FRU FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.49Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fru OCA], [https://pdbe.org/8fru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fru RCSB], [https://www.ebi.ac.uk/pdbsum/8fru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fru ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/V6TF11_GIAIN V6TF11_GIAIN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure of the G. lamblia 80S ribosome bound to tRNA, mRNA, and the antibiotic emetine by cryo-electron microscopy, to an overall resolution of 2.49 A. The structure reveals rapidly evolving protein and nucleotide regions, differences in the peptide exit tunnel, and likely altered ribosome quality control pathways. Examination of translation initiation factor binding sites suggests these interactions are conserved despite a divergent initiation mechanism. Highlighting the potential of G. lamblia to resolve conserved biological principles; our structure reveals the interactions of the translation inhibitor emetine with the ribosome and mRNA, thus providing insight into the mechanism of action for this widely used antibiotic. Our work defines key questions in G. lamblia and motivates future experiments to explore the diversity of eukaryotic gene regulation.

-	~~Authors: Eiler, D.R~~., Wimberly~~, B.T.~~, Bilodeau, ~~D.Y.~~, Rissland, O.S.~~, Kieft, J~~.S.	+	The Giardia lamblia ribosome structure reveals divergence in several biological pathways and the mode of emetine function.,Eiler DR, Wimberly BT, Bilodeau DY, Taliaferro JM, Reigan P, Rissland OS, Kieft JS Structure. 2024 Jan 11:S0969-2126(23)00479-3. doi: 10.1016/j.str.2023.12.015. PMID:38242118<ref>PMID:38242118</ref>

-	~~Description: 60S subunit~~ of the ~~Giardia lamblia 80S ribosome~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Eiler, D.R~~]]	+	<div class="pdbe-citations 8fru" style="background-color:#fffaf0;"></div>
-	[[Category: Bilodeau~~, D.Y~~]]	+	== References ==
-	[[Category: ~~Wimberly, B.T~~]]	+	<references/>
-	[[Category: Kieft~~, J.S~~]]	+	__TOC__
-	[[Category: Rissland~~, O.S~~]]	+	</StructureSection>
		+	[[Category: Giardia intestinalis assemblage A]]
		+	[[Category: Large Structures]]
		+	[[Category: Bilodeau DY]]
		+	[[Category: Eiler DR]]
		+	[[Category: Kieft JS]]
		+	[[Category: Rissland OS]]
		+	[[Category: Wimberly BT]]

OCA at 10:05, 22 February 2023

2023-02-22T10:05:29Z

←Older revision		Revision as of 10:05, 22 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fru is ON HOLD	+	The entry 8fru is ON HOLD until Paper Publication

	Authors: Eiler, D.R., Wimberly, B.T., Bilodeau, D.Y., Rissland, O.S., Kieft, J.S.		Authors: Eiler, D.R., Wimberly, B.T., Bilodeau, D.Y., Rissland, O.S., Kieft, J.S.

OCA: Protected "8fru" [edit=sysop:move=sysop]

2023-01-18T05:11:52Z

Protected "8fru" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 18 January 2023

OCA: New page: '''Unreleased structure''' The entry 8fru is ON HOLD Authors: Eiler, D.R., Wimberly, B.T., Bilodeau, D.Y., Rissland, O.S., Kieft, J.S. Description: 60S subunit of the Giardia lamblia 8...

2023-01-18T05:11:50Z

New page: '''Unreleased structure''' The entry 8fru is ON HOLD Authors: Eiler, D.R., Wimberly, B.T., Bilodeau, D.Y., Rissland, O.S., Kieft, J.S. Description: 60S subunit of the Giardia lamblia 8...

New page

'''Unreleased structure'''

The entry 8fru is ON HOLD

Authors: Eiler, D.R., Wimberly, B.T., Bilodeau, D.Y., Rissland, O.S., Kieft, J.S.

Description: 60S subunit of the Giardia lamblia 80S ribosome
[[Category: Unreleased Structures]]
[[Category: Eiler, D.R]]
[[Category: Bilodeau, D.Y]]
[[Category: Wimberly, B.T]]
[[Category: Kieft, J.S]]
[[Category: Rissland, O.S]]