OCA at 05:43, 31 May 2023

2023-05-31T05:43:37Z

←Older revision		Revision as of 05:43, 31 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8ftf is ~~ON HOLD~~	+	==CryoEM strucutre of 33-mer RBM3 of the Salmonella MS-ring==
		+	<StructureSection load='8ftf' size='340' side='right'caption='[[8ftf]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ftf]] is a 33 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FTF FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ftf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ftf OCA], [https://pdbe.org/8ftf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ftf RCSB], [https://www.ebi.ac.uk/pdbsum/8ftf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ftf ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY] The M ring may be actively involved in energy transduction.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The flagellar motor supports bacterial chemotaxis, a process that allows bacteria to move in response to their environment. A central feature of this motor is the MS-ring, which is composed entirely of repeats of the FliF subunit. This MS-ring is critical for the assembly and stability of the flagellar switch and the entire flagellum. Despite multiple independent cryoEM structures of the MS-ring, there remains a debate about the stoichiometry and organization of the ring-building motifs (RBMs). Here, we report the cryoEM structure of a Salmonella MS-ring that was purified from the assembled flagellar switch complex (MSC-ring). We term this the 'post-assembly' state. Using 2D class averages, we show that under these conditions, the post-assembly MS-ring can contain 32, 33, or 34 FliF subunits, with 33 being the most common. RBM3 has a single location with C32, C33, or C34 symmetry. RBM2 is found in two locations with RBM2inner having C21 or C22 symmetry and an RBM2outer-RBM1 having C11 symmetry. Comparison to previously reported structures identifies several differences. Most strikingly, we find that the membrane domain forms 11 regions of discrete density at the base of the structure rather than a contiguous ring, although density could not be unambiguously interpreted. We further find density in some previously unresolved areas, and we assigned amino acids to those regions. Finally, we find differences in interdomain angles in RBM3 that affect the diameter of the ring. Together, these investigations support a model of the flagellum with structural plasticity, which may be important for flagellar assembly and function.

-	~~Authors: Singh, P.S~~., ~~Nakagawa, T.~~, Cecchini, G~~., Iverson~~, T.M.	+	CryoEM structure of a post-assembly MS-ring reveals plasticity in stoichiometry and conformation.,Singh PK, Cecchini G, Nakagawa T, Iverson TM PLoS One. 2023 May 19;18(5):e0285343. doi: 10.1371/journal.pone.0285343. , eCollection 2023. PMID:37205674<ref>PMID:37205674</ref>

-	~~Description: CryoEM strucutre of 33-mer RBM3~~ of the ~~Salmonella MS~~-~~ring~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Nakagawa, T~~]]	+	<div class="pdbe-citations 8ftf" style="background-color:#fffaf0;"></div>
-	[[Category: Iverson~~, T.M~~]]	+	== References ==
-	[[Category: ~~Cecchini, G~~]]	+	<references/>
-	[[Category: Singh~~, P.S~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
		+	[[Category: Cecchini G]]
		+	[[Category: Iverson TM]]
		+	[[Category: Nakagawa T]]
		+	[[Category: Singh PS]]

OCA: Protected "8ftf" [edit=sysop:move=sysop]

2023-01-18T05:16:10Z

Protected "8ftf" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 18 January 2023

OCA: New page: '''Unreleased structure''' The entry 8ftf is ON HOLD Authors: Singh, P.S., Nakagawa, T., Cecchini, G., Iverson, T.M. Description: CryoEM strucutre of 33-mer RBM3 of the Salmonella MS-r...

2023-01-18T05:16:08Z

New page: '''Unreleased structure''' The entry 8ftf is ON HOLD Authors: Singh, P.S., Nakagawa, T., Cecchini, G., Iverson, T.M. Description: CryoEM strucutre of 33-mer RBM3 of the Salmonella MS-r...

New page

'''Unreleased structure'''

The entry 8ftf is ON HOLD

Authors: Singh, P.S., Nakagawa, T., Cecchini, G., Iverson, T.M.

Description: CryoEM strucutre of 33-mer RBM3 of the Salmonella MS-ring
[[Category: Unreleased Structures]]
[[Category: Nakagawa, T]]
[[Category: Iverson, T.M]]
[[Category: Cecchini, G]]
[[Category: Singh, P.S]]

8ftf - Revision history

OCA at 05:43, 31 May 2023

OCA: Protected "8ftf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ftf is ON HOLD Authors: Singh, P.S., Nakagawa, T., Cecchini, G., Iverson, T.M. Description: CryoEM strucutre of 33-mer RBM3 of the Salmonella MS-r...