OCA at 12:33, 4 October 2023

2023-10-04T12:33:03Z

←Older revision		Revision as of 12:33, 4 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8fyh is ~~ON HOLD until Paper~~ Publication	+	==G4 RNA-mediated PRC2 dimer==
		+	<StructureSection load='8fyh' size='340' side='right'caption='[[8fyh]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fyh]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FYH FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fyh OCA], [https://pdbe.org/8fyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fyh RCSB], [https://www.ebi.ac.uk/pdbsum/8fyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fyh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AEBP2_HUMAN AEBP2_HUMAN] DNA-binding transcriptional repressor. May interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.<ref>PMID:15225548</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polycomb repressive complex 2 (PRC2) silences genes through trimethylation of histone H3K27. PRC2 associates with numerous precursor messenger RNAs (pre-mRNAs) and long noncoding RNAs (lncRNAs) with a binding preference for G-quadruplex RNA. In this work, we present a 3.3-A-resolution cryo-electron microscopy structure of PRC2 bound to a G-quadruplex RNA. Notably, RNA mediates the dimerization of PRC2 by binding both protomers and inducing a protein interface composed of two copies of the catalytic subunit EZH2, thereby blocking nucleosome DNA interaction and histone H3 tail accessibility. Furthermore, an RNA-binding loop of EZH2 facilitates the handoff between RNA and DNA, another activity implicated in PRC2 regulation by RNA. We identified a gain-of-function mutation in this loop that activates PRC2 in zebrafish. Our results reveal mechanisms for RNA-mediated regulation of a chromatin-modifying enzyme.

-	~~Authors: Jiarui~~, J~~.S.~~, ~~Vignesh~~, V.K.	+	Structural basis for inactivation of PRC2 by G-quadruplex RNA.,Song J, Gooding AR, Hemphill WO, Love BD, Robertson A, Yao L, Zon LI, North TE, Kasinath V, Cech TR Science. 2023 Sep 22;381(6664):1331-1337. doi: 10.1126/science.adh0059. Epub 2023 , Sep 21. PMID:37733873<ref>PMID:37733873</ref>

-	~~Description: G4 RNA~~-~~mediated PRC2 dimer~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Jiarui, J.S~~]]	+	<div class="pdbe-citations 8fyh" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Vignesh,~~ V.K]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Kasinath V]]
		+	[[Category: Song J]]

OCA at 05:11, 8 February 2023

2023-02-08T05:11:46Z

←Older revision		Revision as of 05:11, 8 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fyh is ON HOLD	+	The entry 8fyh is ON HOLD until Paper Publication

	Authors: Jiarui, J.S., Vignesh, V.K.		Authors: Jiarui, J.S., Vignesh, V.K.

OCA: Protected "8fyh" [edit=sysop:move=sysop]

2023-02-01T09:32:04Z

Protected "8fyh" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:32, 1 February 2023

OCA: New page: '''Unreleased structure''' The entry 8fyh is ON HOLD Authors: Jiarui, J.S., Vignesh, V.K. Description: G4 RNA-mediated PRC2 dimer Category: Unreleased Structures [[Category: Jiarui...

2023-02-01T09:32:03Z

New page: '''Unreleased structure''' The entry 8fyh is ON HOLD Authors: Jiarui, J.S., Vignesh, V.K. Description: G4 RNA-mediated PRC2 dimer Category: Unreleased Structures [[Category: Jiarui...

New page

'''Unreleased structure'''

The entry 8fyh is ON HOLD

Authors: Jiarui, J.S., Vignesh, V.K.

Description: G4 RNA-mediated PRC2 dimer
[[Category: Unreleased Structures]]
[[Category: Jiarui, J.S]]
[[Category: Vignesh, V.K]]

8fyh - Revision history

OCA at 12:33, 4 October 2023

OCA at 05:11, 8 February 2023

OCA: Protected "8fyh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8fyh is ON HOLD Authors: Jiarui, J.S., Vignesh, V.K. Description: G4 RNA-mediated PRC2 dimer Category: Unreleased Structures [[Category: Jiarui...