8fyo - Revision history

OCA at 04:06, 25 May 2023

2023-05-25T04:06:23Z

←Older revision		Revision as of 04:06, 25 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8fyo is ~~ON HOLD~~	+	==MicroED structure of Proteinase K from lamellae milled from multiple plasma sources==
		+	<StructureSection load='8fyo' size='340' side='right'caption='[[8fyo]], [[Resolution\|resolution]] 1.39Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fyo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FYO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fyo OCA], [https://pdbe.org/8fyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fyo RCSB], [https://www.ebi.ac.uk/pdbsum/8fyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fyo ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystallizing G protein-coupled receptors (GPCRs) in lipidic cubic phase (LCP) often yields crystals suited for the cryogenic electron microscopy (cryoEM) method microcrystal electron diffraction (MicroED). However, sample preparation is challenging. Embedded crystals cannot be targeted topologically. Here, we use an integrated fluorescence light microscope (iFLM) inside of a focused ion beam and scanning electron microscope (FIB-SEM) to identify fluorescently labeled GPCR crystals. Crystals are targeted using the iFLM and LCP is milled using a plasma focused ion beam (pFIB). The optimal ion source for preparing biological lamellae is identified using standard crystals of proteinase K. Lamellae prepared using either argon or xenon produced the highest quality data and structures. MicroED data are collected from the milled lamellae and the structures are determined. This study outlines a robust approach to identify and mill membrane protein crystals for MicroED and demonstrates plasma ion-beam milling is a powerful tool for preparing biological lamellae.

-	~~Authors:~~ Martynowycz~~, M.W.~~, Shiriaeva, A., Clabbers~~, M.T.B.~~, Nicolas~~, W.J.~~, Weaver~~, S.J.~~, Hattne, J., Gonen, T.	+	A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals.,Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T Nat Commun. 2023 Feb 25;14(1):1086. doi: 10.1038/s41467-023-36733-4. PMID:36841804<ref>PMID:36841804</ref>

-	~~Description: MicroED structure~~ of ~~Proteinase K from lamellae milled from multiple plasma sources~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Shiriaeva, A~~]]	+	<div class="pdbe-citations 8fyo" style="background-color:#fffaf0;"></div>
-	[[Category: Gonen, T]]	+	== References ==
-	[[Category: ~~Martynowycz, M.W~~]]	+	<references/>
-	[[Category: ~~Weaver, S.J~~]]	+	__TOC__
-	[[Category: Nicolas~~, W.J~~]]	+	</StructureSection>
-	[[Category: ~~Clabbers, M.T.B~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hattne, J~~]]	+	[[Category: Parengyodontium album]]
		+	[[Category: Clabbers MTB]]
		+	[[Category: Gonen T]]
		+	[[Category: Hattne J]]
		+	[[Category: Martynowycz MW]]
		+	[[Category: Nicolas WJ]]
		+	[[Category: Shiriaeva A]]
		+	[[Category: Weaver SJ]]

OCA at 05:02, 10 May 2023

2023-05-10T05:02:13Z

←Older revision		Revision as of 05:02, 10 May 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fyo is ON HOLD ~~until Paper Publication~~	+	The entry 8fyo is ON HOLD

	Authors: Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T.		Authors: Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T.
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	Description: MicroED structure of Proteinase K from lamellae milled from multiple plasma sources		Description: MicroED structure of Proteinase K from lamellae milled from multiple plasma sources
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Martynowycz, M.W]]
	[[Category: Shiriaeva, A]]		[[Category: Shiriaeva, A]]
-	[[Category: Hattne, J]]
	[[Category: Gonen, T]]		[[Category: Gonen, T]]
		+	[[Category: Martynowycz, M.W]]
	[[Category: Weaver, S.J]]		[[Category: Weaver, S.J]]
	[[Category: Nicolas, W.J]]		[[Category: Nicolas, W.J]]
	[[Category: Clabbers, M.T.B]]		[[Category: Clabbers, M.T.B]]
		+	[[Category: Hattne, J]]

OCA at 04:27, 2 March 2023

2023-03-02T04:27:22Z

←Older revision		Revision as of 04:27, 2 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fyo is ON HOLD	+	The entry 8fyo is ON HOLD until Paper Publication

	Authors: Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T.		Authors: Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T.

OCA: Protected "8fyo" [edit=sysop:move=sysop]

2023-02-01T09:32:34Z

Protected "8fyo" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:32, 1 February 2023

OCA: New page: '''Unreleased structure''' The entry 8fyo is ON HOLD Authors: Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T. Description: MicroE...

2023-02-01T09:32:33Z

New page: '''Unreleased structure''' The entry 8fyo is ON HOLD Authors: Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T. Description: MicroE...

New page

'''Unreleased structure'''

The entry 8fyo is ON HOLD

Authors: Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T.

Description: MicroED structure of Proteinase K from lamellae milled from multiple plasma sources
[[Category: Unreleased Structures]]
[[Category: Martynowycz, M.W]]
[[Category: Shiriaeva, A]]
[[Category: Hattne, J]]
[[Category: Gonen, T]]
[[Category: Weaver, S.J]]
[[Category: Nicolas, W.J]]
[[Category: Clabbers, M.T.B]]