OCA at 14:06, 18 October 2023

2023-10-18T14:06:01Z

←Older revision		Revision as of 14:06, 18 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8g1b is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of polyreactive 2G02 human Fab==
		+	<StructureSection load='8g1b' size='340' side='right'caption='[[8g1b]], [[Resolution\|resolution]] 1.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8g1b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G1B FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g1b OCA], [https://pdbe.org/8g1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g1b RCSB], [https://www.ebi.ac.uk/pdbsum/8g1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g1b ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To become specialized binders, antibodies undergo a process called affinity maturation to maximize their binding affinity. Despite this process, some antibodies retain low-affinity binding to diverse epitopes in a phenomenon called polyreactivity. Here we seek to understand the molecular basis of this polyreactivity in antibodies. Our results highlight that polyreactive antigen-binding fragments (Fabs) bind their targets with low affinities, comparable to T cell receptor recognition of autologous classical major histocompatibility complex. Extensive mutagenic studies find no singular amino acid residue or biochemical property responsible for polyreactive interaction, suggesting that polyreactive antibodies use multiple strategies for engagement. Finally, our crystal structures and all-atom molecular dynamics simulations of polyreactive Fabs show increased rigidity compared to their monoreactive relatives, forming a neutral and accessible platform for diverse antigens to bind. Together, these data support a cooperative strategy of rigid neutrality in establishing the polyreactive status of an antibody molecule.

-	~~Authors:~~ Borowska, ~~M.T.~~, ~~Adams~~, E.J.	+	Biochemical and biophysical characterization of natural polyreactivity in antibodies.,Borowska MT, Boughter CT, Bunker JJ, Guthmiller JJ, Wilson PC, Roux B, Bendelac A, Adams EJ Cell Rep. 2023 Oct 5;42(10):113190. doi: 10.1016/j.celrep.2023.113190. PMID:37804505<ref>PMID:37804505</ref>

-	~~Description: Crystal structure~~ of ~~polyreactive 2G02 human Fab~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Borowska, M.T~~]]	+	<div class="pdbe-citations 8g1b" style="background-color:#fffaf0;"></div>
-	[[Category: Adams~~, E.J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Adams EJ]]
		+	[[Category: Borowska MT]]

OCA at 05:44, 8 March 2023

2023-03-08T05:44:16Z

←Older revision		Revision as of 05:44, 8 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8g1b is ON HOLD	+	The entry 8g1b is ON HOLD until Paper Publication

	Authors: Borowska, M.T., Adams, E.J.		Authors: Borowska, M.T., Adams, E.J.

OCA: Protected "8g1b" [edit=sysop:move=sysop]

2023-02-08T05:19:15Z

Protected "8g1b" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:19, 8 February 2023

OCA: New page: '''Unreleased structure''' The entry 8g1b is ON HOLD Authors: Borowska, M.T., Adams, E.J. Description: Crystal structure of polyreactive 2G02 human Fab [[Category: Unreleased Structure...

2023-02-08T05:19:14Z

New page: '''Unreleased structure''' The entry 8g1b is ON HOLD Authors: Borowska, M.T., Adams, E.J. Description: Crystal structure of polyreactive 2G02 human Fab [[Category: Unreleased Structure...

New page

'''Unreleased structure'''

The entry 8g1b is ON HOLD

Authors: Borowska, M.T., Adams, E.J.

Description: Crystal structure of polyreactive 2G02 human Fab
[[Category: Unreleased Structures]]
[[Category: Borowska, M.T]]
[[Category: Adams, E.J]]

8g1b - Revision history

OCA at 14:06, 18 October 2023

OCA at 05:44, 8 March 2023

OCA: Protected "8g1b" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8g1b is ON HOLD Authors: Borowska, M.T., Adams, E.J. Description: Crystal structure of polyreactive 2G02 human Fab [[Category: Unreleased Structure...