OCA at 05:28, 9 August 2023

2023-08-09T05:28:16Z

←Older revision		Revision as of 05:28, 9 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8g64 is ~~ON HOLD until Paper~~ Publication	+	==Heme-bound flavodoxin FldH from Fusobacterium nucleatum==
		+	<StructureSection load='8g64' size='340' side='right'caption='[[8g64]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8g64]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusobacterium_nucleatum Fusobacterium nucleatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G64 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G64 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g64 OCA], [https://pdbe.org/8g64 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g64 RCSB], [https://www.ebi.ac.uk/pdbsum/8g64 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g64 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8RI14_FUSNN Q8RI14_FUSNN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fusobacterium nucleatum is an opportunistic oral pathogen that is associated with various cancers. To fulfill its essential need for iron, this anaerobe will express heme uptake machinery encoded at a single genetic locus. The heme uptake operon includes HmuW, a class C radical SAM-dependent methyltransferase that degrades heme anaerobically to release Fe(2+) and a linear tetrapyrrole called anaerobilin. The last gene in the operon, hmuF encodes a member of the flavodoxin superfamily of proteins. We discovered that HmuF and a paralog, FldH, bind tightly to both FMN and heme. The structure of Fe(3+)-heme-bound FldH (1.6 A resolution) reveals a helical cap domain appended to the âº/beta core of the flavodoxin fold. The cap creates a hydrophobic binding cleft that positions the heme planar to the si-face of the FMN isoalloxazine ring. The ferric heme iron is hexacoordinated to His134 and a solvent molecule. In contrast to flavodoxins, FldH and HmuF do not stabilize the FMN semiquinone but instead cycle between the FMN oxidized and hydroquinone states. We show that heme-loaded HmuF and heme-loaded FldH traffic heme to HmuW for degradation of the protoporphyrin ring. Both FldH and HmuF then catalyze multiple reductions of anaerobilin through hydride transfer from the FMN hydroquinone. The latter activity eliminates the aromaticity of anaerobilin and the electrophilic methylene group that was installed through HmuW turnover. Hence, HmuF provides a protected path for anaerobic heme catabolism, offering F. nucleatum a competitive advantage in the colonization of anoxic sites of the human body.

-	~~Authors~~:	+	A new member of the flavodoxin superfamily from Fusobacterium nucleatum that functions in heme trafficking and reduction of anaerobilin.,McGregor AK, Chan ACK, Schroeder MD, Do LTM, Saini G, Murphy MEP, Wolthers KR J Biol Chem. 2023 Jul;299(7):104902. doi: 10.1016/j.jbc.2023.104902. Epub 2023 , Jun 10. PMID:37302554<ref>PMID:37302554</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8g64" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fusobacterium nucleatum]]
		+	[[Category: Large Structures]]
		+	[[Category: Chan AC]]
		+	[[Category: Murphy ME]]
		+	[[Category: Wolthers KR]]

OCA: Protected "8g64" [edit=sysop:move=sysop]

2023-02-22T10:22:47Z

Protected "8g64" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:22, 22 February 2023

OCA: New page: '''Unreleased structure''' The entry 8g64 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2023-02-22T10:22:44Z

New page: '''Unreleased structure''' The entry 8g64 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8g64 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

8g64 - Revision history

OCA at 05:28, 9 August 2023

OCA: Protected "8g64" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8g64 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures