OCA at 09:40, 21 June 2023

2023-06-21T09:40:01Z

←Older revision		Revision as of 09:40, 21 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8gul is ~~ON HOLD until Paper~~ Publication	+	==Chitin-active AA10 LPMO (GbpA) complexed with Cu(II) from Vibrio campbellii==
		+	<StructureSection load='8gul' size='340' side='right'caption='[[8gul]], [[Resolution\|resolution]] 2.44Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8gul]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_campbellii_ATCC_BAA-1116 Vibrio campbellii ATCC BAA-1116]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GUL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gul OCA], [https://pdbe.org/8gul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gul RCSB], [https://www.ebi.ac.uk/pdbsum/8gul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gul ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GBPA_VIBC1 GBPA_VIBC1] Probably interacts with GlcNAc residues. May promote attachment to both epithelial cell surfaces and chitin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vibrio spp. play a crucial role in the global recycling of the highly abundant recalcitrant biopolymer chitin in marine ecosystems through their ability to secrete chitin-degrading enzymes to efficiently hydrolyse chitinous materials and use them as their major carbon source. In this study, the first crystal structures of a complete four-domain chitin-active AA10 lytic polysaccharide monooxygenase from the chitinolytic bacterium Vibrio campbellii type strain ATCC BAA-1116 are reported. The crystal structures of apo and copper-bound VhLPMO10A were resolved as homodimers with four distinct domains: an N-terminal AA10 catalytic (CatD) domain connected to a GlcNAc-binding (GbpA_2) domain, followed by a module X domain and a C-terminal carbohydrate-binding module (CBM73). Size-exclusion chromatography and small-angle X-ray scattering analysis confirmed that VhLPMO10A exists as a monomer in solution. The active site of VhLPMO10A is located on the surface of the CatD domain, with three conserved residues (His1, His98 and Phe170) forming the copper(II)-binding site. Metal-binding studies using synchrotron X-ray absorption spectroscopy and X-ray fluorescence, together with electron paramagnetic resonance spectroscopy, gave consistently strong copper(II) signals in the protein samples, confirming that VhLPMO10A is a copper-dependent enzyme. ITC binding data showed that VhLPMO10A could bind various divalent cations but bound most strongly to copper(II) ions, with a K(d) of 0.1 +/- 0.01 microM. In contrast, a K(d) of 1.9 nM was estimated for copper(I) ions from redox-potential measurements. The presence of ascorbic acid is essential for H(2)O(2) production in the reaction catalysed by VhLPMO10A. MALDI-TOF MS identified VhLPMO10A as a C1-specific LPMO, generating oxidized chitooligosaccharide products with different degrees of polymerization (DP2(ox)-DP8(ox)). This new member of the chitin-active AA10 LPMOs could serve as a powerful biocatalyst in biofuel production from chitin biomass.

-	~~Authors: Zhou~~, Y., Robinson, R.C., ~~Suginta, W~~.	+	Structural and binding studies of a new chitin-active AA10 lytic polysaccharide monooxygenase from the marine bacterium Vibrio campbellii.,Zhou Y, Wannapaiboon S, Prongjit M, Pornsuwan S, Sucharitakul J, Kamonsutthipaijit N, Robinson RC, Suginta W Acta Crystallogr D Struct Biol. 2023 Jun 1;79(Pt 6):479-497. doi: , 10.1107/S2059798323003261. Epub 2023 May 30. PMID:37259836<ref>PMID:37259836</ref>

-	~~Description: Chitin~~-~~active AA10 LPMO (GbpA) complexed with Cu(II) from Vibrio campbellii~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Suginta, W~~]]	+	<div class="pdbe-citations 8gul" style="background-color:#fffaf0;"></div>
-	[[Category: Robinson~~, R.C~~]]	+	== References ==
-	[[Category: Zhou, Y]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Vibrio campbellii ATCC BAA-1116]]
		+	[[Category: Robinson RC]]
		+	[[Category: Suginta W]]
		+	[[Category: Zhou Y]]

OCA at 10:28, 22 February 2023

2023-02-22T10:28:40Z

←Older revision		Revision as of 10:28, 22 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8gul is ON HOLD	+	The entry 8gul is ON HOLD until Paper Publication

	Authors: Zhou, Y., Robinson, R.C., Suginta, W.		Authors: Zhou, Y., Robinson, R.C., Suginta, W.

OCA at 05:20, 8 February 2023

2023-02-08T05:20:30Z

←Older revision		Revision as of 05:20, 8 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8gul is ON HOLD ~~until Paper Publication~~	+	The entry 8gul is ON HOLD

	Authors: Zhou, Y., Robinson, R.C., Suginta, W.		Authors: Zhou, Y., Robinson, R.C., Suginta, W.

OCA at 04:09, 26 October 2022

2022-10-26T04:09:25Z

←Older revision		Revision as of 04:09, 26 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8gul is ON HOLD	+	The entry 8gul is ON HOLD until Paper Publication

	Authors: Zhou, Y., Robinson, R.C., Suginta, W.		Authors: Zhou, Y., Robinson, R.C., Suginta, W.

OCA: Protected "8gul" [edit=sysop:move=sysop]

2022-09-21T06:42:41Z

Protected "8gul" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:42, 21 September 2022

OCA: New page: '''Unreleased structure''' The entry 8gul is ON HOLD Authors: Zhou, Y., Robinson, R.C., Suginta, W. Description: Chitin-active AA10 LPMO (GbpA) complexed with Cu(II) from Vibrio campbe...

2022-09-21T06:42:40Z

New page: '''Unreleased structure''' The entry 8gul is ON HOLD Authors: Zhou, Y., Robinson, R.C., Suginta, W. Description: Chitin-active AA10 LPMO (GbpA) complexed with Cu(II) from Vibrio campbe...

New page