OCA at 13:08, 1 November 2023

2023-11-01T13:08:24Z

←Older revision		Revision as of 13:08, 1 November 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8h91 is ~~ON HOLD until 2024~~-10-24	+	==Crystal structure of SARS-CoV-2 spike receptor-binding domain in complex with nanobody N19==
-		+	<StructureSection load='8h91' size='340' side='right'caption='[[8h91]], [[Resolution\|resolution]] 3.07Å' scene=''>
-	~~Authors~~: ~~Zhang~~, Y.T., Li, J., ~~Zhang~~, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8h91]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H91 FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~SARS~~-~~CoV~~-~~2 spike receptor~~-~~binding domain~~ in ~~complex with nanobody N19~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.07Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h91 OCA], [https://pdbe.org/8h91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h91 RCSB], [https://www.ebi.ac.uk/pdbsum/8h91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h91 ProSAT]</span></td></tr>
-	[[Category: ~~Zhang, Y.T~~]]	+	</table>
-	[[Category: Li, J]]	+	== Function ==
-	[[Category: Zhang, J]]	+	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Vicugna pacos]]
		+	[[Category: Li J]]
		+	[[Category: Zhang J]]
		+	[[Category: Zhang YT]]

OCA at 05:50, 7 December 2022

2022-12-07T05:50:38Z

←Older revision		Revision as of 05:50, 7 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8h91 is ON HOLD	+	The entry 8h91 is ON HOLD until 2024-10-24

	Authors: Zhang, Y.T., Li, J., Zhang, J.		Authors: Zhang, Y.T., Li, J., Zhang, J.

OCA: Protected "8h91" [edit=sysop:move=sysop]

2022-11-03T05:35:46Z

Protected "8h91" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:35, 3 November 2022

OCA: New page: '''Unreleased structure''' The entry 8h91 is ON HOLD Authors: Zhang, Y.T., Li, J., Zhang, J. Description: Crystal structure of SARS-CoV-2 spike receptor-binding domain in complex with ...

2022-11-03T05:35:45Z

New page: '''Unreleased structure''' The entry 8h91 is ON HOLD Authors: Zhang, Y.T., Li, J., Zhang, J. Description: Crystal structure of SARS-CoV-2 spike receptor-binding domain in complex with ...

New page

'''Unreleased structure'''

The entry 8h91 is ON HOLD

Authors: Zhang, Y.T., Li, J., Zhang, J.

Description: Crystal structure of SARS-CoV-2 spike receptor-binding domain in complex with nanobody N19
[[Category: Unreleased Structures]]
[[Category: Zhang, Y.T]]
[[Category: Li, J]]
[[Category: Zhang, J]]

8h91 - Revision history

OCA at 13:08, 1 November 2023

OCA at 05:50, 7 December 2022

OCA: Protected "8h91" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8h91 is ON HOLD Authors: Zhang, Y.T., Li, J., Zhang, J. Description: Crystal structure of SARS-CoV-2 spike receptor-binding domain in complex with ...