OCA at 12:39, 26 July 2023

2023-07-26T12:39:24Z

←Older revision		Revision as of 12:39, 26 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8h9y is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in calcium ion condition==
		+	<StructureSection load='8h9y' size='340' side='right'caption='[[8h9y]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8h9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliarcobacter_butzleri Aliarcobacter butzleri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H9Y FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1N7:CHAPSO'>1N7</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PX4:1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PX4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h9y OCA], [https://pdbe.org/8h9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h9y RCSB], [https://www.ebi.ac.uk/pdbsum/8h9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h9y ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A8EVM5_ALIB4 A8EVM5_ALIB4]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Divalent cation block is observed in various tetrameric ion channels. For blocking, a divalent cation is thought to bind in the ion pathway of the channel, but such block has not yet been directly observed. So, the behaviour of these blocking divalent cations remains still uncertain. Here, we elucidated the mechanism of the divalent cation block by reproducing the blocking effect into NavAb, a well-studied tetrameric sodium channel. Our crystal structures of NavAb mutants show that the mutations increasing the hydrophilicity of the inner vestibule of the pore domain enable a divalent cation to stack on the ion pathway. Furthermore, non-equilibrium molecular dynamics simulation showed that the stacking calcium ion repel sodium ion at the bottom of the selectivity filter. These results suggest the primary process of the divalent cation block mechanism in tetrameric cation channels.

-	~~Authors: Irie~~, K., Oda, Y.	+	The structural basis of divalent cation block in a tetrameric prokaryotic sodium channel.,Irie K, Oda Y, Sumikama T, Oshima A, Fujiyoshi Y Nat Commun. 2023 Jul 15;14(1):4236. doi: 10.1038/s41467-023-39987-0. PMID:37454189<ref>PMID:37454189</ref>

-	~~Description: Crystal structure~~ of ~~voltage~~-~~gated sodium channel NavAb N49K~~/~~L176Q mutant in calcium ion condition~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Oda, Y~~]]	+	<div class="pdbe-citations 8h9y" style="background-color:#fffaf0;"></div>
-	[[Category: Irie, K]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aliarcobacter butzleri]]
		+	[[Category: Large Structures]]
		+	[[Category: Irie K]]
		+	[[Category: Oda Y]]

OCA at 03:51, 31 May 2023

2023-05-31T03:51:18Z

←Older revision		Revision as of 03:51, 31 May 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8h9y is ON HOLD	+	The entry 8h9y is ON HOLD until Paper Publication

	Authors: Irie, K., Oda, Y.		Authors: Irie, K., Oda, Y.

OCA at 05:27, 18 May 2023

2023-05-18T05:27:10Z

←Older revision		Revision as of 05:27, 18 May 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8h9y is ON HOLD ~~until Paper Publication~~	+	The entry 8h9y is ON HOLD

	Authors: Irie, K., Oda, Y.		Authors: Irie, K., Oda, Y.

OCA at 08:21, 24 November 2022

2022-11-24T08:21:53Z

←Older revision		Revision as of 08:21, 24 November 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8h9y is ON HOLD	+	The entry 8h9y is ON HOLD until Paper Publication

	Authors: Irie, K., Oda, Y.		Authors: Irie, K., Oda, Y.

OCA: Protected "8h9y" [edit=sysop:move=sysop]

2022-11-03T05:37:42Z

Protected "8h9y" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 3 November 2022

OCA: New page: '''Unreleased structure''' The entry 8h9y is ON HOLD Authors: Irie, K., Oda, Y. Description: Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in calcium ion co...

2022-11-03T05:37:41Z

New page: '''Unreleased structure''' The entry 8h9y is ON HOLD Authors: Irie, K., Oda, Y. Description: Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in calcium ion co...

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