OCA at 11:26, 1 February 2023

2023-02-01T11:26:11Z

←Older revision		Revision as of 11:26, 1 February 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8hi2 is ~~ON HOLD until Paper~~ Publication	+	==Structure of EV71 VLP frozen at -183 degree embedded in crystalline ice==
		+	<StructureSection load='8hi2' size='340' side='right'caption='[[8hi2]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8hi2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HI2 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hi2 OCA], [https://pdbe.org/8hi2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hi2 RCSB], [https://www.ebi.ac.uk/pdbsum/8hi2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hi2 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2D2CKV5_HE71 A0A2D2CKV5_HE71]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	For cryoelectron microscopy (cryo-EM), high cooling rates have been required for preparation of protein samples to vitrify the surrounding water and avoid formation of damaging crystalline ice. Whether and how crystalline ice affects single-particle cryo-EM is still unclear. Here, single-particle cryo-EM was used to analyze three-dimensional structures of various proteins and viruses embedded in crystalline ice formed at various cooling rates. Low cooling rates led to shrinkage deformation and density distortions on samples having loose structures. Higher cooling rates reduced deformations. Deformation-free proteins in crystalline ice were obtained by modifying the freezing conditions, and reconstructions from these samples revealed a marked improvement over vitreous ice. This procedure also increased the efficiency of cryo-EM structure determinations and was essential for high-resolution reconstructions.

-	~~Authors: Shi~~, H., Wu, C., Zhang, X.	+	Addressing compressive deformation of proteins embedded in crystalline ice.,Shi H, Wu C, Zhang X Structure. 2022 Dec 14:S0969-2126(22)00487-7. doi: 10.1016/j.str.2022.12.001. PMID:36586403<ref>PMID:36586403</ref>

-	~~Description: Structure~~ of ~~EV71 VLP frozen at~~ -~~183 degree embedded in crystalline ice~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Wu, C~~]]	+	<div class="pdbe-citations 8hi2" style="background-color:#fffaf0;"></div>
-	[[Category: Shi, H]]	+	== References ==
-	[[Category: Zhang, X]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Enterovirus A71]]
		+	[[Category: Large Structures]]
		+	[[Category: Shi H]]
		+	[[Category: Wu C]]
		+	[[Category: Zhang X]]

OCA at 06:58, 21 December 2022

2022-12-21T06:58:56Z

←Older revision		Revision as of 06:58, 21 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hi2 is ON HOLD	+	The entry 8hi2 is ON HOLD until Paper Publication

	Authors: Shi, H., Wu, C., Zhang, X.		Authors: Shi, H., Wu, C., Zhang, X.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Wu, C]]		[[Category: Wu, C]]
-	[[Category: Zhang, X]]
	[[Category: Shi, H]]		[[Category: Shi, H]]
		+	[[Category: Zhang, X]]

OCA: Protected "8hi2" [edit=sysop:move=sysop]

2022-11-24T08:30:31Z

Protected "8hi2" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:30, 24 November 2022

OCA: New page: '''Unreleased structure''' The entry 8hi2 is ON HOLD Authors: Shi, H., Wu, C., Zhang, X. Description: Structure of EV71 VLP frozen at -183 degree embedded in crystalline ice [[Category...

2022-11-24T08:30:30Z

New page: '''Unreleased structure''' The entry 8hi2 is ON HOLD Authors: Shi, H., Wu, C., Zhang, X. Description: Structure of EV71 VLP frozen at -183 degree embedded in crystalline ice [[Category...

New page

'''Unreleased structure'''

The entry 8hi2 is ON HOLD

Authors: Shi, H., Wu, C., Zhang, X.

Description: Structure of EV71 VLP frozen at -183 degree embedded in crystalline ice
[[Category: Unreleased Structures]]
[[Category: Wu, C]]
[[Category: Zhang, X]]
[[Category: Shi, H]]

8hi2 - Revision history

OCA at 11:26, 1 February 2023

OCA at 06:58, 21 December 2022

OCA: Protected "8hi2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8hi2 is ON HOLD Authors: Shi, H., Wu, C., Zhang, X. Description: Structure of EV71 VLP frozen at -183 degree embedded in crystalline ice [[Category...