OCA at 23:11, 27 December 2023

2023-12-27T23:11:26Z

←Older revision		Revision as of 23:11, 27 December 2023
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-	'''Unreleased structure'''

-	The entry ~~8hj1~~ is ~~ON HOLD~~ ~~until sometime~~ in the ~~future~~	+	==GPR21(wt) and Gs complex==
		+	<StructureSection load='8hj1' size='340' side='right'caption='[[8hj1]], [[Resolution\|resolution]] 3.27Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8hj1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HJ1 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.27Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hj1 OCA], [https://pdbe.org/8hj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hj1 RCSB], [https://www.ebi.ac.uk/pdbsum/8hj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hj1 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/GNAS2_HUMAN GNAS2_HUMAN] Pseudopseudohypoparathyroidism;Pseudohypoparathyroidism type 1A;Progressive osseous heteroplasia;Polyostotic fibrous dysplasia;Monostotic fibrous dysplasia;Pseudohypoparathyroidism type 1C;Pseudohypoparathyroidism type 1B;McCune-Albright syndrome. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. Most affected individuals have defects in methylation of the gene. In some cases microdeletions involving the STX16 appear to cause loss of methylation at exon A/B of GNAS, resulting in PHP1B. Paternal uniparental isodisomy have also been observed. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GNAS2_HUMAN GNAS2_HUMAN] Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed:17110384). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed:26206488, PubMed:8702665). GNAS functions downstream of several GPCRs, including beta-adrenergic receptors (PubMed:21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed:12391161).<ref>PMID:12391161</ref> <ref>PMID:17110384</ref> <ref>PMID:21488135</ref> <ref>PMID:26206488</ref> <ref>PMID:8702665</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GPR21 is a class-A orphan G protein-coupled receptor (GPCR) and a potential therapeutic target for type 2 diabetes and other metabolic disorders. This receptor shows high basal activity in coupling to multiple G proteins in the absence of any known endogenous agonist or synthetic ligand. Here, we present the structures of ligand-free human GPR21 bound to heterotrimeric miniGs and miniG15 proteins, respectively. We identified an agonist-like motif in extracellular loop 2 (ECL2) that occupies the orthosteric pocket and promotes receptor activation. A side pocket that may be employed as a new ligand binding site was also uncovered. Remarkably, G protein binding is accommodated by a flexible cytoplasmic portion of transmembrane helix 6 (TM6) which adopts little or undetectable outward movement. These findings will enable the design of modulators for GPR21 for understanding its signal transduction and exploring opportunity for deorphanization.

-	~~Authors: Chen, B~~., Lin, X., Xu, F.	+	Cryo-EM structures of orphan GPR21 signaling complexes.,Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F Nat Commun. 2023 Jan 13;14(1):216. doi: 10.1038/s41467-023-35882-w. PMID:36639690<ref>PMID:36639690</ref>

-	~~Description~~: ~~GPR21(wt) and Gs complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Xu, F~~]]	+	<div class="pdbe-citations 8hj1" style="background-color:#fffaf0;"></div>
-	[[Category: Lin, X]]	+	== References ==
-	[[Category: ~~Chen, B~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Chen B]]
		+	[[Category: Lin X]]
		+	[[Category: Xu F]]

OCA at 06:52, 6 December 2023

2023-12-06T06:52:11Z

←Older revision		Revision as of 06:52, 6 December 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hj1 is ON HOLD	+	The entry 8hj1 is ON HOLD until sometime in the future

	Authors: Chen, B., Lin, X., Xu, F.		Authors: Chen, B., Lin, X., Xu, F.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Xu, F]]		[[Category: Xu, F]]
-	[[Category: Chen, B]]
	[[Category: Lin, X]]		[[Category: Lin, X]]
		+	[[Category: Chen, B]]

OCA: Protected "8hj1" [edit=sysop:move=sysop]

2022-11-30T08:51:31Z

Protected "8hj1" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:51, 30 November 2022

OCA: New page: '''Unreleased structure''' The entry 8hj1 is ON HOLD Authors: Chen, B., Lin, X., Xu, F. Description: GPR21(wt) and Gs complex Category: Unreleased Structures Category: Xu, F [[...

2022-11-30T08:51:28Z

New page: '''Unreleased structure''' The entry 8hj1 is ON HOLD Authors: Chen, B., Lin, X., Xu, F. Description: GPR21(wt) and Gs complex Category: Unreleased Structures Category: Xu, F [[...

New page

'''Unreleased structure'''

The entry 8hj1 is ON HOLD

Authors: Chen, B., Lin, X., Xu, F.

Description: GPR21(wt) and Gs complex
[[Category: Unreleased Structures]]
[[Category: Xu, F]]
[[Category: Chen, B]]
[[Category: Lin, X]]

8hj1 - Revision history

OCA at 23:11, 27 December 2023

OCA at 06:52, 6 December 2023

OCA: Protected "8hj1" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8hj1 is ON HOLD Authors: Chen, B., Lin, X., Xu, F. Description: GPR21(wt) and Gs complex Category: Unreleased Structures Category: Xu, F [[...