OCA at 14:07, 18 October 2023

2023-10-18T14:07:28Z

←Older revision		Revision as of 14:07, 18 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8hlg is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of MoaE==
		+	<StructureSection load='8hlg' size='340' side='right'caption='[[8hlg]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8hlg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HLG FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hlg OCA], [https://pdbe.org/8hlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hlg RCSB], [https://www.ebi.ac.uk/pdbsum/8hlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hlg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9RR88_DEIRA Q9RR88_DEIRA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Molybdenum ions are covalently bound to molybdenum pterin (MPT) to produce molybdenum cofactor (Moco), a compound essential for the catalytic activity of molybdenum enzymes, which is involved in a variety of biological functions. MoaE is the large subunit of MPT synthase and plays a key role in Moco synthesis. Here, we investigated the function of MoaE in Deinococcus radiodurans (DrMoaE) in vitro and in vivo, demonstrating that the protein contributed to the extreme resistance of D. radiodurans. The crystal structure of DrMoaE was determined by 1.9 A resolution. DrMoaE was shown to be a dimer and the dimerization disappeared after Arg110 had been mutated. The deletion of drmoaE resulted in sensitivity to DNA damage stress and a slower growth rate in D. radiodurans. The increase in drmoaE transcript levels the and accumulation of intracellular reactive oxygen species levels under oxidative stress suggested that it was involved in the antioxidant process in D. radiodurans. In addition, treatment with the base analog 6-hydroxyaminopurine decreased survival and increased intracellular mutation rates in drmoaE deletion mutant strains. Our results reveal that MoaE plays a role in response to external stress mainly through oxidative stress resistance mechanisms in D. radiodurans.

-	~~Authors~~:	+	MoaE Is Involved in Response to Oxidative Stress in Deinococcus radiodurans.,Cai J, Zhang M, Chen Z, Zhao Y, Xu H, Tian B, Wang L, Hua Y Int J Mol Sci. 2023 Jan 26;24(3):2441. doi: 10.3390/ijms24032441. PMID:36768763<ref>PMID:36768763</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8hlg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Deinococcus radiodurans R1]]
		+	[[Category: Large Structures]]
		+	[[Category: Cai J]]
		+	[[Category: Zhao Y]]

OCA at 06:08, 11 January 2023

2023-01-11T06:08:13Z

←Older revision		Revision as of 06:08, 11 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hlg is ON HOLD	+	The entry 8hlg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8hlg" [edit=sysop:move=sysop]

2022-12-07T06:00:38Z

Protected "8hlg" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:00, 7 December 2022

OCA: New page: '''Unreleased structure''' The entry 8hlg is ON HOLD Authors: Description: Category: Unreleased Structures

2022-12-07T06:00:36Z

New page: '''Unreleased structure''' The entry 8hlg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8hlg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8hlg - Revision history

OCA at 14:07, 18 October 2023

OCA at 06:08, 11 January 2023

OCA: Protected "8hlg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8hlg is ON HOLD Authors: Description: Category: Unreleased Structures