OCA at 07:33, 3 May 2023

2023-05-03T07:33:45Z

←Older revision		Revision as of 07:33, 3 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8hsg is ~~ON HOLD until Paper~~ Publication	+	==Thermus thermophilus RNA polymerase elongation complex==
		+	<StructureSection load='8hsg' size='340' side='right'caption='[[8hsg]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8hsg]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/DNA_molecule DNA molecule], [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HSG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hsg OCA], [https://pdbe.org/8hsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hsg RCSB], [https://www.ebi.ac.uk/pdbsum/8hsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hsg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transcription termination is an essential step in transcription by RNA polymerase (RNAP) and crucial for gene regulation. For many bacterial genes, transcription termination is mediated by the adenosine triphosphate-dependent RNA translocase/helicase Rho, which causes RNA/DNA dissociation from the RNAP elongation complex (EC). However, the structural basis of the interplay between Rho and RNAP remains obscure. Here, we report the cryo-electron microscopy structure of the Thermus thermophilus RNAP EC engaged with Rho. The Rho hexamer binds RNAP through the carboxyl-terminal domains, which surround the RNA exit site of RNAP, directing the nascent RNA seamlessly from the RNA exit to its central channel. The beta-flap tip at the RNA exit is critical for the Rho-dependent RNA release, and its deletion causes an alternative Rho-RNAP binding mode, which is irrelevant to termination. The Rho binding site overlaps with the binding sites of other macromolecules, such as ribosomes, providing a general basis of gene regulation.

-	~~Authors~~:	+	Structural basis of the transcription termination factor Rho engagement with transcribing RNA polymerase from Thermus thermophilus.,Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI Sci Adv. 2023 Feb 10;9(6):eade7093. doi: 10.1126/sciadv.ade7093. Epub 2023 Feb 8. PMID:36753546<ref>PMID:36753546</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8hsg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA molecule]]
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Thermus thermophilus HB8]]
		+	[[Category: Ehara H]]
		+	[[Category: Murayama Y]]
		+	[[Category: Sekine S]]

OCA at 05:20, 18 January 2023

2023-01-18T05:20:15Z

←Older revision		Revision as of 05:20, 18 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hsg is ON HOLD	+	The entry 8hsg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8hsg" [edit=sysop:move=sysop]

2022-12-28T06:28:57Z

Protected "8hsg" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:28, 28 December 2022

OCA: New page: '''Unreleased structure''' The entry 8hsg is ON HOLD Authors: Description: Category: Unreleased Structures

2022-12-28T06:28:56Z

New page: '''Unreleased structure''' The entry 8hsg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8hsg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8hsg - Revision history

OCA at 07:33, 3 May 2023

OCA at 05:20, 18 January 2023

OCA: Protected "8hsg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8hsg is ON HOLD Authors: Description: Category: Unreleased Structures