8i5a - Revision history

OCA at 19:52, 29 May 2024

2024-05-29T19:52:58Z

←Older revision		Revision as of 19:52, 29 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8i5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_sp. Burkholderia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I5A FirstGlance]. <br>		<table><tr><td colspan='2'>[[8i5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_sp. Burkholderia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I5A FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i5a OCA], [https://pdbe.org/8i5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i5a RCSB], [https://www.ebi.ac.uk/pdbsum/8i5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i5a ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i5a OCA], [https://pdbe.org/8i5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i5a RCSB], [https://www.ebi.ac.uk/pdbsum/8i5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i5a ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:54, 15 March 2023

2023-03-15T09:54:34Z

←Older revision		Revision as of 09:54, 15 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8i5a is ~~ON HOLD until Paper~~ Publication	+	==N-acetyl-(R)-beta-phenylalanine acylase, 2.75 angstrom resolution==
		+	<StructureSection load='8i5a' size='340' side='right'caption='[[8i5a]], [[Resolution\|resolution]] 2.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8i5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_sp. Burkholderia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I5A FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i5a OCA], [https://pdbe.org/8i5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i5a RCSB], [https://www.ebi.ac.uk/pdbsum/8i5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i5a ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	N-Acetyl-(R)-beta-phenylalanine acylase is an enzyme that hydrolyzes the amide bond of N-acetyl-(R)-beta-phenylalanine to produce enantiopure (R)-beta-phenylalanine. In previous studies, Burkholderia sp. AJ110349 and Variovorax sp. AJ110348 were isolated as (R)-enantiomer-specific N-acetyl-(R)-beta-phenylalanine acylase-producing organisms and the properties of the native enzyme from Burkholderia sp. AJ110349 were characterized. In this study, structural analyses were carried out in order to investigate the structure-function relationships of the enzymes derived from both organisms. The recombinant N-acetyl-(R)-beta-phenylalanine acylases were crystallized by the hanging-drop vapor-diffusion method under multiple crystallization solution conditions. The crystals of the Burkholderia enzyme belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 112.70-112.97, c = 341.50-343.32 A, and were likely to contain two subunits in the asymmetric unit. The crystal structure was solved by the Se-SAD method, suggesting that two subunits in the asymmetric unit form a dimer. Each subunit was composed of three domains, and they showed structural similarity to the corresponding domains of the large subunit of N,N-dimethylformamidase from Paracoccus sp. strain DMF. The crystals of the Variovorax enzyme grew as twinned crystals and were not suitable for structure determination. Using size-exclusion chromatography with online static light-scattering analysis, the N-acetyl-(R)-beta-phenylalanine acylases were clarified to be dimeric in solution.

-	~~Authors: Kato~~, Y., ~~Natsume~~, R.	+	Expression, purification and crystallization of N-acetyl-(R)-beta-phenylalanine acylases derived from Burkholderia sp. AJ110349 and Variovorax sp. AJ110348 and structure determination of the Burkholderia enzyme.,Kato Y, Kawasaki H, Nakamatsu T, Matsuda N, Natsume R Acta Crystallogr F Struct Biol Commun. 2023 Mar 1;79(Pt 3):70-78. doi: , 10.1107/S2053230X23000730. Epub 2023 Feb 23. PMID:36862095<ref>PMID:36862095</ref>

-	~~Description: N-acetyl-(R)-beta-phenylalanine acylase~~, 2.~~75 angstrom resolution~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Natsume, R~~]]	+	<div class="pdbe-citations 8i5a" style="background-color:#fffaf0;"></div>
-	[[Category: Kato, Y]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Burkholderia sp]]
		+	[[Category: Large Structures]]
		+	[[Category: Kato Y]]
		+	[[Category: Natsume R]]

OCA: Protected "8i5a" [edit=sysop:move=sysop]

2023-02-01T09:38:26Z

Protected "8i5a" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:38, 1 February 2023

OCA: New page: '''Unreleased structure''' The entry 8i5a is ON HOLD until Paper Publication Authors: Kato, Y., Natsume, R. Description: N-acetyl-(R)-beta-phenylalanine acylase, 2.75 angstrom resoluti...

2023-02-01T09:38:25Z

New page: '''Unreleased structure''' The entry 8i5a is ON HOLD until Paper Publication Authors: Kato, Y., Natsume, R. Description: N-acetyl-(R)-beta-phenylalanine acylase, 2.75 angstrom resoluti...

New page

'''Unreleased structure'''

The entry 8i5a is ON HOLD until Paper Publication

Authors: Kato, Y., Natsume, R.

Description: N-acetyl-(R)-beta-phenylalanine acylase, 2.75 angstrom resolution
[[Category: Unreleased Structures]]
[[Category: Natsume, R]]
[[Category: Kato, Y]]