OCA at 08:01, 6 December 2023

2023-12-06T08:01:14Z

←Older revision		Revision as of 08:01, 6 December 2023
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-	'''Unreleased structure'''

-	The ~~entry~~ 8i6p is ~~ON HOLD until Paper~~ Publication	+	==The cryo-EM structure of OsCyc1 tetramer state==
		+	<StructureSection load='8i6p' size='340' side='right'caption='[[8i6p]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8i6p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I6P FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.5Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i6p OCA], [https://pdbe.org/8i6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i6p RCSB], [https://www.ebi.ac.uk/pdbsum/8i6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i6p ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CPS4_ORYSJ CPS4_ORYSJ] Catalyzes the conversion of geranylgeranyl diphosphate to the phytoalexin precursor syn-copalyl diphosphate (PubMed:15341631, PubMed:15255861, PubMed:23621683). Required for the biosynthesis of momilactones that exude from roots and act as allelochemicals against lowland weeds in paddy soil (PubMed:23621683).<ref>PMID:15255861</ref> <ref>PMID:15341631</ref> <ref>PMID:23621683</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The large superfamily of labdane-related diterpenoids is defined by the cyclization of linear geranylgeranyl pyrophosphate (GGPP), catalyzed by copalyl diphosphate synthases (CPSs) to form the basic decalin core, the copalyl diphosphates (CPPs). Three stereochemically distinct CPPs have been found in plants, namely (+)-CPP, ent-CPP and syn-CPP. Here, we used X-ray crystallography and cryo-EM methods to describe different oligomeric structures of a syn-copalyl diphosphate synthase from Oryza sativa (OsCyc1), and provided a cryo-EM structure of OsCyc1(D367A) mutant in complex with the substrate GGPP. Further analysis showed that tetramers are the dominant form of OsCyc1 in solution and are not necessary for enzyme activity in vitro. Through rational design, we identified an OsCyc1 mutant that can generate ent-CPP in addition to syn-CPP. Our work provides a structural and mechanistic basis for comparing different CPSs and paves the way for further enzyme design to obtain diterpene derivatives with specific chirality.

-	~~Authors~~:	+	Structural and functional investigations of syn-copalyl diphosphate synthase from Oryza sativa.,Ma X, Xu H, Tong Y, Luo Y, Dong Q, Jiang T Commun Chem. 2023 Nov 6;6(1):240. doi: 10.1038/s42004-023-01042-w. PMID:37932442<ref>PMID:37932442</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8i6p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Oryza sativa Japonica Group]]
		+	[[Category: Dong QH]]
		+	[[Category: Jiang T]]
		+	[[Category: Luo YF]]
		+	[[Category: Ma XL]]
		+	[[Category: Tong YR]]
		+	[[Category: Xu HF]]

OCA at 04:37, 2 March 2023

2023-03-02T04:37:58Z

←Older revision		Revision as of 04:37, 2 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8i6p is ON HOLD	+	The entry 8i6p is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8i6p" [edit=sysop:move=sysop]

2023-02-08T05:27:10Z

Protected "8i6p" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:27, 8 February 2023

OCA: New page: '''Unreleased structure''' The entry 8i6p is ON HOLD Authors: Description: Category: Unreleased Structures

2023-02-08T05:27:09Z

New page: '''Unreleased structure''' The entry 8i6p is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8i6p is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8i6p - Revision history

OCA at 08:01, 6 December 2023

OCA at 04:37, 2 March 2023

OCA: Protected "8i6p" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8i6p is ON HOLD Authors: Description: Category: Unreleased Structures